Effects of supplying omega-3 polyunsaturated fatty acids to gilts after weaning on metabolism and ovarian gene expression1

Otte, Marina Vianna; Moreira, Fabiana; Bianchi, Ivan; Oliveira, Juahil Martins de; Mendes, Ricardo E.; Haas, Cristina; Anciuti, Andréia Nobre; Rovani, Monique Tomazele; Gasperin, Bernardo Garziera; Lucia, Thomaz

doi:10.1093/jas/sky419

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…Although all of our diet reversals were able to at least partially restore HFD-induced gene dysregulation, each of the three dietary interventions also invoked a unique ovarian gene expression profile, highlighting the impact diet has on ovarian gene expression. Others have also shown that changes in diet alter ovarian gene expression including HFDs (69), omega-3 FA supplementation (70), and maternal low protein diets (71). Interestingly, we have previously shown that HFD alters ovarian gene expression regardless of obesity (7).…”

Section: Discussionmentioning

confidence: 65%

High-fat diet-induced dysregulation of ovarian gene expression is restored with chronic omega-3 fatty acid supplementation

Hohos

Elliott

Cho

et al. 2020

Molecular and Cellular Endocrinology

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Chronic high-fat diet (HFD) consumption causes ovarian dysfunction in rodents. Acute dietary treatment with docosahexaenoic acid (DHA) increases oocyte quality and ovarian reserve at advanced reproductive age. We hypothesized that DHA supplementation after HFD exposure reverses HFD-induced ovarian defects. We conducted a dietary intervention with reversal to chow, DHA-supplemented chow, or DHA-supplemented HFD after HFD consumption. After 10 weeks, HFD-fed mice had impaired estrous cyclicity, decreased primordial follicles, and altered ovarian expression of 24 genes compared to chow controls. Diet reversal to either chow or chow+DHA restored estrous cyclicity, however only chow+DHA appeared to mitigated the impact of HFD on ovarian reserve. All dietary interventions restored HFD-dysregulated gene expression to chow levels. We found no association between follicular fluid DHA levels and ovarian reserve. In conclusion our data suggest some benefit of DHA supplementation after HFD, particularly in regards to ovarian gene expression, however complete restoration of ovarian function was not achieved.

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Section: Discussionmentioning

confidence: 65%

High-fat diet-induced dysregulation of ovarian gene expression is restored with chronic omega-3 fatty acid supplementation

Hohos

Elliott

Cho

et al. 2020

Molecular and Cellular Endocrinology

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“…In animals fed restricted food for a considerable amount of time, the number of dominant follicles in the ovaries was minimal, resulting in low estrogen levels. Low cholesterol levels in this study also caused low estrogen levels because total cholesterol influences ovulatory steroidogenesis [ 34 ]. A previous study found that low blood GLU and cholesterol levels reduce LH pulses during follicular development, and LH functions as a factor in the formation of androgen hormones in granulosa cells to be converted into estrogen hormones.…”

Section: Discussionmentioning

confidence: 99%

Effect of short time precise dietary energy–protein in reproductive parameters of local crossbred dairy goats

Sitaresmi¹,

Hudaya²,

Kumala³

et al. 2023

J Adv Vet Anim Res

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Objective: This study aimed to establish and focus on the implications of precise energy and protein levels on reproductive performance. This study based on field facts showed that excessive feed intake, especially protein intake, to increase productivity will lead to some decreased reproductive efficiency through disruption of reproductive hormonal compound biosynthesis or increasing blood urea nitrogen (BUN), which has rarely been studied. Materials and Methods: Healthy and mature Saanen crossbred goats raised by traditional farmers (2–4 years old; ± 45 kg) were divided into three experimental groups [T0 = basal feed according to the feed provided by farmers, T1 = protein and energy balanced feed according to National Research Council (NRC) (±5%), T2 = feed >20% excess protein, and excess energy from NRC] ( n = 75). The factorial method was used in the experimental design with a post-hoc least significant difference test. Results: The data showed a significant difference ( p < 0.05) in the biochemical or hormonal parameters between the control and the treatment groups. Also, T1 showed leads in any blood profile and reproductive hormone parameters such as total protein (79.6 ± 5.3 gm/dl), cholesterol (3.26 ± 0.47 mmol/l), progesterone (1.79 ± 2.21 ng/ml), and estrogen (47.85 ± 5.51 pg/ml), as well as the lowest levels of BUN (11.6 ± 1.59 mmol/l) and cortisol (25.07 ± 14.85 ng/ml) levels. T1 had the highest reproductive potential after treatment. Conclusion: The adverse effects of high and low protein consumption on reproductive hormones seem to be related to the blood profile and ovarian function, as hormone concentrations change significantly and lead to reproductive impairment. The data showed that balanced nutrient levels (5%/T1) resulted in excellent blood and hormone parameters.

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“…It is important to recognize, however, that diet can affect ovarian function. For example, low protein intake reduces mouse primordial follicle activation, and omega-3 polyunsaturated fatty acids supplements alter mouse follicular cell gene expression [50, 51]. Thus, systematically determining the effect of specific diets on periovarian adipose tissue architecture and lipid profiles could be informative.…”

Section: Discussionmentioning

confidence: 99%

Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue

Dipali

Ferreira

Zhou

et al. 2019

Reprod Biol Endocrinol

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Background Reproductive aging is a robust phenotype that occurs in all females and is characterized by a significant reduction in gamete quantity and quality, which can have negative consequences on both endocrine function and fertility. Age-associated differences in the oocyte, follicle, and ovary have been well-documented, but how the broader environment changes with age is less well understood. Fat is one of the largest organs in the body, and peri-gonadal adipose tissue surrounds the rodent ovary and comprises a local ovarian environment. The goal of this study was to characterize how peri-ovarian adipose tissue changes with advanced reproductive age. Methods We isolated peri-gonadal adipose tissue from two cohorts of CB6F1 mice: reproductively young (6–12 weeks) and reproductively old (14–17 months). A comparative histological analysis was performed to evaluate adipocyte architecture. We then extracted lipids from the tissue and performed multiple reaction monitoring (MRM)-profiling, a mass spectrometry-based method of metabolite profiling, to compare the lipid profiles of peri-gonadal adipose tissue in these age cohorts. Results We found that advanced reproductive age was associated with adipocyte hypertrophy and a corresponding decrease in the number of adipocytes per area. Of the 10 lipid classes examined, triacylglycerols (TAGs) had significantly different profiles between young and old cohorts, despite quantitative analysis revealing a decrease in the total amount of TAGs per weight of peri-gonadal adipose tissue with age. Conclusions These findings pinpoint age-associated physiological changes in peri-gonadal adipose tissue with respect to adipocyte morphology and lipid profiles and lay the foundation for future studies to examine how these alterations may influence both adipocyte and ovarian function. Electronic supplementary material The online version of this article (10.1186/s12958-019-0487-6) contains supplementary material, which is available to authorized users.

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Effects of supplying omega-3 polyunsaturated fatty acids to gilts after weaning on metabolism and ovarian gene expression1

Cited by 8 publications

References 48 publications

High-fat diet-induced dysregulation of ovarian gene expression is restored with chronic omega-3 fatty acid supplementation

High-fat diet-induced dysregulation of ovarian gene expression is restored with chronic omega-3 fatty acid supplementation

Effect of short time precise dietary energy–protein in reproductive parameters of local crossbred dairy goats

Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue

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