Effects of surface properties and bioactivation of biomaterials on endothelial cells

Monchaux, Emmanuelle; Vermette, Patrick

doi:10.2741/s61

Cited by 20 publications

(5 citation statements)

References 117 publications

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“…Finally, we studied about the application of this examination. Vascular grafts that promote endothelialization have recently been developed [ 16 , 39 , 40 ]. In order to ensure the quality of these devices, an examination that checks the endothelialization treatment was properly done is required.…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Endothelialization Test of Biomaterials Using Immortalized Endothelial Cells

et al. 2016

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Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. To assess endothelialization of materials in vitro, primary ECs are generally used, although the characteristics of these cells vary among the donors and change with time in culture. Recently, primary cell lines immortalized by transduction of simian vacuolating virus 40 large T antigen or human telomerase reverse transcriptase have been developed. To determine whether immortalized ECs can substitute for primary ECs in material testing, we investigated endothelialization on biocompatible polymers using three lots of primary human umbilical vein endothelial cells (HUVEC) and immortalized microvascular ECs, TIME-GFP. Attachment to and growth on polymer surfaces were comparable between cell types, but results were more consistent with TIME-GFP. Our findings indicate that TIME-GFP is more suitable for in vitro endothelialization testing of biomaterials.

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Section: Resultsmentioning

confidence: 99%

“…Primary ECs have been generally used to investigate whether coated bioactive molecules can promote endothelialization in vitro [ 16 – 18 ]. However, the characteristics of these cells vary among donors, and change with time in culture [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Endothelialization Test of Biomaterials Using Immortalized Endothelial Cells

et al. 2016

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“…The great importance of EC coverage of synthetic surfaces in tissue engineering has been shown in vitro [18–21] as well as in animal studies [9,22]. One way to improve endothelialization of cell-repellent surfaces such as PU was nanoupgrading with titaniumcarboxonitride using PACVD technology [12,23].…”

Section: Discussionmentioning

confidence: 99%

In vitro Endothelialization and Platelet Adhesion on Titaniferous Upgraded Polyether and Polycarbonate Polyurethanes

Lehle

Zimmermann

et al. 2014

Materials

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Polycarbonateurethanes (PCU) and polyetherurethanes (PEU) are used for medical devices, however their bio- and haemocompatibility is limited. In this study, the effect of titaniferous upgrading of different polyurethanes on the bio- and haemocompatibility was investigated by endothelial cell (EC) adhesion/proliferation and platelet adhesion (scanning electron microscopy), respectively. There was no EC adhesion/proliferation and only minor platelet adhesion on upgraded and pure PCU (Desmopan). PEUs (Texin 985, Tecothane 1085, Elastollan 1180A) differed in their cyto- and haemocompatibility. While EC adhesion depended on the type of PEU, any proliferative activity was inhibited. Additional titaniferous upgrading of PEU induced EC proliferation and increased metabolic activity. However, adherent ECs were significantly activated. While Texin was highly thrombotic, only small amounts of platelets adhered onto Tecothane and Elastollan. Additional titaniferous upgrading reduced thrombogenicity of Texin, preserved haemocompatibility of Elastollan, and increased platelet activation/aggregation on Tecothane. In conclusion, none of the PUs was cytocompatible; only titaniferous upgrading allowed EC proliferation and metabolism on PEUs. Haemocompatibility depended on the type of PU.

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“…Modifying surface properties of bioactive materials have been shown to induce the adsorption of proteins and the subsequent adhesion of cells. For example, cells on stiff surfaces formed a more organized cytoskeleton, actin stress fibers, and increased focal adhesion, resulting in higher adhesion strength than on soft surfaces [ 192 ]. The results indicate the potential of the approach for promoting the adhesion of vascular endothelial cells for vascularization to facilitate SwG regeneration.…”

Section: Bioactive Biomaterial-based Microenvironmental Re-establishm...mentioning

confidence: 99%

“…For example, the in vivo delivery of VEGF, FGF, and PDGF promoted vascularization and increased blood flow [ 193 , 194 ]. Besides, several ECM components, including fibronectin (Fn), type I and Type IV collagen, were used to coat the surface of biomaterials to enhance the adhesion and migration of endothelial cells by interacting with their receptors, such as integrin α5β1, αvβ3, α1β1, α2β1 [ 192 ]. These bioactive molecules are expected to be used to modify biomaterials and thus promote SwG regeneration.…”

Section: Bioactive Biomaterial-based Microenvironmental Re-establishm...mentioning

confidence: 99%

Bioactive materials for in vivo sweat gland regeneration

Yang

Xiong

et al. 2024

Bioactive Materials

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Effects of surface properties and bioactivation of biomaterials on endothelial cells

Cited by 20 publications

References 117 publications

In Vitro Endothelialization Test of Biomaterials Using Immortalized Endothelial Cells

In Vitro Endothelialization Test of Biomaterials Using Immortalized Endothelial Cells

In vitro Endothelialization and Platelet Adhesion on Titaniferous Upgraded Polyether and Polycarbonate Polyurethanes

Bioactive materials for in vivo sweat gland regeneration

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