Effects of surface sterilisation with green synthesised silver nanoparticles on Lamiaceae seeds

Abstract

Nanoparticles have been used in many areas of biotechnology. In this study, an alternative surface sterilisation method was established for plant tissue cultures. Silver nanoparticles synthesised via green synthesis were used for the surface sterilisation of Lamiaceae seeds ( - Large Leaf Italian, var. Water extracts of dried rhizomes and flowers were utilised in the bioreduction of silver ions. The seeds were exposed to 0, 1, 7, 14 and 28 day-old colloidal solutions of silver nanoparticles and their effects on germination and surface sterilisation were determined. Fresh (0 and 1 day-old) colloidal solutions of silver nanoparticles were found very effective on surface sterilisation (100%). Moreover, they showed no negative effect on both germination and morphology of plantlets. It was shown that silver nanoparticles can be used as a surface sterilisation agent and they have no adverse effects on seed germination and in vitro plantlet growth.

Silver Nanoparticles Synthesized with Extracts of Leaves of Raphanus sativus L, Beta vulgaris L, and Ocimum basilicum and Its Application in Seed Disinfection

“…With both seeds, no signifcant diferences were observed between the treatments with the AgNPs and the traditional disinfection treatment. In another work, similar results were obtained, a 100% disinfection of Lamiacea seeds with AgNPs synthesized from extracts of Alkanna tinctorum rhizomes roots and Syzygium aromaticum fowers was achieved [35]. Our results support the idea that bisynthesized AgNPs could be used to disinfect seeds for in vitro cultivation.…”

Ameliorated Antibacterial and Antioxidant Properties by Trichoderma harzianum Mediated Green Synthesis of Silver Nanoparticles

“…The biosynthesis of silver nanoparticles (AgNPs) has been proven by many researchers to be the best alternative when compared with physical and chemical methods of nanoparticle synthesis, as it is amore eco-friendly and cost-effective route [5]. The AgNPs are the most significant NPs used worldwide as they have several applications in clothing, dentistry, photography, coating of surgical devices, food industry, catalysis, optics, electronics, mirrors, prostheses, textiles, wound dressing, cosmetics and agriculture [6,7]. AgNPs synthesized by green methods are described to have various biological functions, making them useful in antidiabetic, anti-inflammatory, anticancer, antimicrobial, antiplasmodial, vector control and sensor applications, and also act as catalysts [8,9].…”

Tohumların Yeşil Sterilizasyonu: Peganum harmala ve Rumex acetosella Tohumlarının Yüzeysel Sterilizasyonunda Biyosentetik Gümüş Nanopartiküllerin Kullanılması

“…Biyosentetik gümüş nanopartiküllerin bitki hücre ve doku kültürlerinde kullanımlarına yönelik çalışmalar son zamanlarda artış göstermiştir. Bitki hücre ve dokularının gelişimi üzerine olumlu etkilerinin yanı sıra tohumların ve bitki eksplantlarının yüzeysel sterilizasyonunda kullanımına dair başarılı sonuçlar da elde edilmektedir (Nartop 2016, Nartop 2018a, Nartop 2018b. Biyosentetik gümüş nanopartiküllerin etkileri uygulama sürelerine ve uygulama dozlarına göre farklılık göstermektedir.…”

“…Gümüş nanopartiküller tıbbi uygulamalarda cihazların ve yüzeylerin sterilizasyonunda kullanılmaktadırlar. Benzer şekilde, bitkilere ait eksplantların ve tohumların yüzeysel sterilizasyonunda da başarıyla kullanılmaktadırlar (Nartop 2016;Nartop 2018a, Nartop 2018b…”