Effects of Surface Structure and Chemical Composition of Binary Ti Alloys on Cell Differentiation

Abstract

Binary Ti alloys containing Fe, Mo, V and Zr were micro-arc oxidized and hydrothermally treated to obtain micro-and nano-porous layers. This study aimed to investigate cell differentiation on micro and micro/nanoporous oxide layers of Ti alloys. The properties of the porous layer formed on Ti alloys were characterized by X-ray diffraction pattern, microstructural and elemental analyses and inductively coupled plasma mass spectrometry (ICP-MS) method. The MTT assay, total protein production and alkaline phosphatase (ALPase) activity were evaluated using human osteoblast-like cells (MG-63). Microporous structures of micro-arc oxidized Ti alloys were changed to micro/nanoporous surfaces after hydrothermal treatment. Micro/nanoporous surfaces consisted of acicular TiO 2 nanoparticles and micron-sized hydroxyapatite particles. From ICP and MTT tests, the Mo and V ions released from porous oxide layers were positive for cell viability, while the released Fe ions were negative for cell viability. Although the micro/nanoporous surfaces led to a lower total protein content than the polished and microporous Ti surfaces after cell incubation for 7 days, they caused higher ALPase activities after 7 days and 14 days of incubation except for V-containing microporous surfaces. The micro/nanoporous surfaces of Ti alloys were more efficient in inducing MG-63 cell differentiation.

Effect of Anode Pulse-Width on the Microstructure and Wear Resistance of Microarc Oxidation Coatings

“…These technologies are also not environmentally friendly [6,7]. Microarc oxidation (MAO), namely plasma electrolytic oxidation (PEO) [8], is an effective technique for the surface treatment of aluminum alloys [9,10]. MAO could be used to prepare ceramic-like oxide coatings on the surface of valve metals [11,12].…”

Preparation and Properties of Microarc Oxidation Self-Lubricating Composite Coatings on Aluminum Alloy

“…These technologies are also not environmentally friendly [6,7]. Microarc oxidation (MAO), namely plasma electrolytic oxidation (PEO) [8], emerged as a novel technique with broad application prospects [9,10]. MAO could be used to prepare ceramic-like oxide coatings on the surface of some light metals in-situ through anodic oxidation, thermochemistry, electrochemistry and plasma chemical mechanisms [11,12].…”