Effects of synthetic colloid and crystalloid solutions on hemorheology in vitro and in hemorrhagic shock

Gan, Chunfang; Zhao, Jingxiang; Li, Penglong; Kan, Xuemei; You, Guoxiang; Wang, Ying; Yin, Yujing; Luo, Xin; Zhang, Yuhua; Zhao, Lei; Zhou, Hong

doi:10.1186/s40001-015-0088-6

Cited by 10 publications

(7 citation statements)

References 36 publications

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“…Gel also resulted a higher viscosity than plasma at all shear rates. These data are similar to the result of a previous study reporting that Gel increased the blood viscosity [ 25 ]. Since viscosity at low shear rates indicates the aggregation of red cells, the results in this study are consistent with the finding that Gel induces red cell aggregation [ 26 ].…”

Section: Discussionsupporting

confidence: 92%

Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

You¹,

Li²,

Wang³

et al. 2020

Military Med Res

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Background Plasma expanders are widely used for acute normovolemic hemodilution (ANH). However, existing studies have not focused on large-volume infusion with colloidal plasma expanders, and there is a lack of studies that compare the effects of different plasma expanders. Methods The viscosity, hydrodynamic radius (Rh) and colloid osmotic pressure (COP) of plasma expanders were determined by a cone-plate viscometer, Zetasizer and cut-off membrane, respectively. Sixty male rats were randomized into five groups with Gelofusine (Gel), Hydroxyethyl Starch 200/0.5 (HES200), Hydroxyethyl Starch 130/0.4 (HES130), Hydroxyethyl Starch 40 (HES40), and Dextran40 (Dex40), with 12 rats used in each group to build the ANH model. ANH was performed by the withdrawal of blood and simultaneous infusion of plasma expanders. Acid-base, lactate, blood gas and physiological parameters were detected. Results Gel had a lower intrinsic viscosity than HES200 and HES130 (P < 0.01), but at a low shear rate in a mixture of colloids, red cells and plasma, Gel had a higher viscosity (P < 0.05 or P < 0.01, respectively). For hydroxyethyl starch plasma expanders, the COP at a certain concentration decreases from 11.1 mmHg to 6.1 mmHg with the increase of Rh from 10.7 nm to 20.2 nm. A severe ANH model, with the hematocrit of 40% of the baseline level, was established and accompanied by disturbances in acid-base, lactate and blood gas parameters. At the end of ANH and 60 min afterward, the Dex40 group showed a worse outcome in maintaining the acid-base balance and systemic oxygenation compared to the other groups. The systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) decreased significantly in all groups at the end of ANH. The DBP and MAP in the Dex40 group further decreased 60 min after the end of ANH. During the process of ANH, the Dex40 group showed a drop and recovery in SBP, DBP and MAP. The DBP and MAP in the HES200 group were significantly higher than those in the other groups at some time points (P < 0.05 or P < 0.01). Conclusion Gel had a low intrinsic viscosity but may increase the whole blood viscosity at low shear rates. Rh and COP showed a strong correlation among hydroxyethyl starch plasma expanders. Dex40 showed a worse outcome in maintaining the acid-base balance and systemic oxygenation compared to the other plasma expanders. During the process of ANH, Dex40 displayed a V-shaped recovery pattern for blood pressure, and HES200 had the advantage in sustaining the DBP and MAP at some time points.

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Section: Discussionsupporting

confidence: 92%

Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

You¹,

Li²,

Wang³

et al. 2020

Military Med Res

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“…53 STAT1 activity is also related to the stimulation of IFN-β. 54 This is reflected in the GO analysis where positive regulation of IFN-β production would be attributable to osteopenia in DS-NF1. Taken together, the upregulated expression of STAT1 might play a significant role in osteopenia in DS-NF1.…”

Section: Discussionmentioning

confidence: 98%

Differential Gene Expression Profiles and Pathways Highlight the Role of Osteoimmunology in Neurofibromatosis Type 1–Related Dystrophic Scoliosis With Osteopenia

Li,

Wu,

et al. 2023

Spine

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Study Design: Microarray approach and integrated gene network analysis. Objective: To explore the differential genetic expression profile, Gene Ontology (GO) terms, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in human trabecular bone-derived cells (HTBs) of dystrophic scoliosis secondary to Neurofibromatosis Type 1 (DS-NF1) and compare these to normal controls. Summary of Background Data: The pathogenesis of DS-NF1 and the accompanying generalized osteopenia remain unclear. We hypothesized that HTBs may play a significant role in the etiology and pathogenesis of DS-NF1. Methods: Microarray analysis was used to identify differentially expressed genes (DEGs) of HTBs from DS-NF1 patients compared with those from healthy individuals. Functional and pathway enrichment analysis were implemented through GO and KEGG pathway database. Then, search tool for the retrival of interacting genes/proteins (STRING) database, Cytoscape and Molecular Complex Detection (MCODE) were applied to construct the protein-protein interaction (PPI) network and screen hub genes. Pathway enrichment analysis was further performed for hub genes and gene clusters identified via module analysis. Six potential crucial genes were selected for validation by reverse transcription polymerase chain reaction. Results: Bioinformatic analysis revealed that there are 401 previously unrecognized DEGs (238 up and 163 down-regulated genes) in HTBs from DS-NF1 patients, and they were mainly enriched in the terms of immune response, type I interferon signaling, TNF signaling pathway and RIG-I-like receptor signaling pathway. Five hub genes including STAT1, OASL, IFIH1, IRF7 and MX1 were identified through PPI network, which were mainly enriched in terms of Jak-STAT and RIG-I-like receptor signaling pathway. An independently dysregulated protein cluster containing CCL2, CXCL1, CXCL3, CX3CL1, TLR1 and CXCL12 was also identified via PPI network. This indicated that the upper abnormally expressed genes may play essential roles in DS-NF1 pathogenesis and accompanied osteopenia. Conclusion: Six key genes were identified in the progression of DS-NF1-related osteopenia. Immune response might play a key role in the progression of osteopenia, while a CXCL12-mediated osteogenic effect might play a protective role. Level of Evidence: N/A.

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“…57 Although gelatine has a lower intrinsic viscosity than HES, its effect on red blood cell aggregation leads to increased viscosity in vivo compared to HES. 58 In the microcirculation, relative viscosity is lower than in larger vessels, the so-called Få hraeuseLindqvist effect, and a reduction in haematocrit can, therefore, be of particular influence on capillary blood flow and capillary density (Fig 1). 59,60 Most studies focusing on the effects of different fluid types on viscosity and microcirculatory function are limited to experimental animal studies.…”

Section: Viscosity and Microvascular Perfusionmentioning

confidence: 99%

Choice of fluid type: physiological concepts and perioperative indications

Boer¹,

Bossers²,

Koning³

2018

British Journal of Anaesthesia

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The consensus that i.v. resuscitation fluids should be considered as drugs with specific dose recommendations, contraindications, and side-effects has led to an increased attention for the choice of fluid during perioperative care. In particular, the debate concerning possible adverse effects of unbalanced fluids and hydroxyethyl starches resulted in a re-evaluation of the roles of different fluid types in the perioperative setting. This review provides a concise overview of the current knowledge regarding the efficacy and safety of distinct fluid types for perioperative use. First, basic physiological aspects and possible side-effects are explained. Second, we focus on considerations regarding fluid choice for specific perioperative indications based on an analysis of available randomized controlled trials.

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Effects of synthetic colloid and crystalloid solutions on hemorheology in vitro and in hemorrhagic shock

Cited by 10 publications

References 36 publications

Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

Differential Gene Expression Profiles and Pathways Highlight the Role of Osteoimmunology in Neurofibromatosis Type 1–Related Dystrophic Scoliosis With Osteopenia

Choice of fluid type: physiological concepts and perioperative indications

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