Effects of tannic acid on growth performance, relative organ weight, antioxidative status, and intestinal histomorphology in broilers exposed to aflatoxin B1

Abstract

A total of 480 one-day-old AA broiler chicks were randomly allocated to one of four treatments in a 2 × 2 factorial to investigate the effects of tannic acid (TA) on growth performance, relative organ weight, antioxidant capacity, and intestinal health in broilers dietary exposed to aflatoxin B1 (AFB1). Treatments were as follows: (1) CON, control diet; (2) TA, CON + 250 mg/kg TA; (3) AFB1, CON + 500 μg/kg AFB1; and (4) TA+AFB1, CON + 250 mg/kg TA + 500 μg/kg AFB1. There were 10 replicate pens with 12 broilers per replicate. Dietary AFB1 challenge increased the feed conversion ratio during days 1 to 21 (P < 0.05). The TA in the diet did not show significant effects on the growth performance of broilers during the whole experiment period (P > 0.05). The liver and kidney relative weight was increased in the AF challenge groups compared with the CON (P < 0.05). The addition of TA could alleviate the relative weight increase of liver and kidney caused by AFB1 (P < 0.05). Broilers fed the AFB1 diets had lower activity of glutathione peroxidase, catalase, total superoxide dismutase, S-transferase, and total antioxidant capacity in plasma, liver and jejunum, and greater malondialdehyde content (P < 0.05). Dietary supplemented with 250 mg/kg TA increased the activities of antioxidative enzymes, and decreased malondialdehyde content (P < 0.05). In addition, AFB1 significantly reduced the villus height and crypt depth ratio in the ileum on day 42 (P < 0.05). In conclusion, supplementation with 250 mg/kg TA could partially protect the antioxidant capacity and prevent the enlargement of liver in broilers dietary challenged with 500 μg/kg AFB1.

Effect of Tannic Acid on Antioxidant Function, Immunity, and Intestinal Barrier of Broilers Co-Infected with Coccidia and Clostridium perfringens

“…Consistent with previous findings, we found that Jejunal H 2 O 2 and MDA contents were increased, and T-AOC, SOD, CAT, and GSH-Px levels were decreased by CCP challenge, which indicated that CCP co-infection caused oxidative stress in the broiler. Dietary supplementation with TA has been shown to enhance the activities of intestinal GSH-Px, SOD, and CAT, and to decrease H 2 O 2 and MDA contents, thereby increasing intestinal antioxidant capacity and protecting the intestinal tract from damage caused by oxidative stress [ 9 , 10 , 27 ]. In the present study, we found that dietary supplementation with 1000 mg/kg TA reduced the increase in peroxides caused by CCP challenge and enhanced the activities of jejunal antioxidant enzymes.…”

“…Tannins are natural polyphenolic substances in numerous plants with anti-bacterial, anti-viral, anti-inflammatory, and anti-oxidation functions [ 7 , 8 ]. Research has demonstrated that dietary supplementation with hydrolyzed tannins can reduce oxidative stress and improve antioxidant capacities in broilers [ 9 , 10 ]. Dietary TA can also alleviate tissue inflammation and protect intestinal health by lowering the mRNA levels of tissue inflammation elements and increasing the mRNA levels of intestinal intact proteins in broilers [ 11 , 12 , 13 ].…”

The Role of Supplementing a Complex Phytobiotic Feed Additive Containing (Castanea sativa mill) Extract in Combination with Calcium Butyrate, Zinc–Methionine and Essential Oils on Growth Indicators, Blood Profile and Carcass Quality of Broiler Chickens

“…The use of a single substitute or ideal combinations of various alternatives (a complex of phytobiotics and other nutraceuticals) with the proper management and practice of animal husbandry can play a key role in maximizing productivity and maintaining animal productivity [ 9 , 51 , 52 , 53 , 54 ].…”

“…Blood cells play an essential role in the body of animals; for example, they are responsible for the transport of nutrients, the exchange of gases, provide protection to the body and have the ability to synthesize immune bodies [ 36 , 49 , 50 , 51 , 52 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ]. So, it was found that the introduction of phytobiotics did not significantly affect the morphological parameters of blood, except for the content of leukocytes.…”