Tigernut (Cyperus esculentus L.), native to northeast Africa, belongs to the sedge family (Babiker et al., 2021). It is an important multipurpose crop, its stems and leaves can be used as green feed and knitting materials, and the roots with a fragrant smell are a potential source of essential oils (Duman, 2019;Lasekan, 2013;Ozcan et al., 2021). Tigernut tubers are rich in lipids, proteins, starches, dietary fibers, and vitamins, which are usually applied to develop edible oil, plant milk, and snack food (Clemente-Villalba et al., 2021;Oluwajuyitan & Ijarotimi, 2019).The extraction and physicochemical characterization of tigernut tuber oil have been widely reported (Aljuhaimi, Ghafoor, et al., 2018; Aljuhaimi, Simsek, & Ozcan, 2018). But there are few types of research on tigernut tuber starch (TTS). Manek et al. found that TTS conformed well to United States Pharmacopeia and could be employed as a binder for the production of metronidazole tablets (Manek et al., 2012). It was found that the freeze-thaw stability of TTS was higher than that of potato starch (PS), but its paste clarity was lower (Jing et al., 2012). Neto et al. evaluated the physicochemical properties of TTS and modified it with octenyl succinic anhydride, which improved the resistance of TTS to temperature (Neto et al., 2018). Liu et al. isolated TTS from tigernut tuber meals and cakes remaining after oil extraction and found that the various oil extraction methods had different influences on the ordered structure of starch granules, resulting in the change of functional properties of TTS (Liu et al., 2019). In this study, the preparation, composition, granule morphology, crystal structure, pasting properties, functional properties, and gel properties of tigernut tuber starch (TTS) were systematically investigated. The feasibility of TTS to replace wheat flour (WF) in steamed bread production was also evaluated.