Effects of tea polyphenols on the activities of antioxidant enzymes and the expression of related gene in the leaves of wheat seedlings under salt stress

Abstract

Longchun 30, a new wheat variety, was used to investigate seedling growth, element absorption and antioxidant response under 150 mM NaCl and tea polyphenols (TP) (25 and 100 mg L − 1 ) treatments alone or in combination, thus revealing TP-alleviating mechanism on the salt damage to plants. 150 mM NaCl stress alone inhibited the seedling growth, increased sodium content and reactive oxygen species (ROS) accumulation, but reduced potassium (K) and calcium (Ca) levels at different culture times, thus resulting in the oxidative damage to the leaves. Even though TP treatment alone led to the signi cant increase of ROS generation, TP-treated leaves exhibited the reduction of relative electrical conductivity and no change of malondialdehyde content. Moreover, high TP concentration alone stimulated the seedling growth. In addition, the activities and gene expression of superoxide dismutase, catalase and peroxidase (POD) as well as diamine oxidase and polyamine oxidase were changed to different degrees due to NaCl or TP treatment alone. Further study showed that the presence of TP promoted the seedling growth, increased K + and Ca 2+ contents, and led to the reduction of ROS accumulation. Taken together, salinity-inhibitory effect on the growth of wheat seedlings might be associated with salt-induced imbalance of element content and the increase of oxidative damage resulting from ROS accumulation, while the application of TP effectively alleviated salinity-inhibitory effect on the seedling growth and improved the tolerance of wheat seedlings to salt environment, which might be associated with the increases of K + and Ca 2+ contents as well as the reduction of oxidative damage in the leaves of wheat seedlings under NaCl and TP treatment in combination.

Effects of Exogenous Calcium on Adaptive Growth, Photosynthesis, Ion Homeostasis and Phenolics of Gleditsia sinensis Lam. Plants under Salt Stress

“…However, after different concentrations of CaCl 2 are added, the plant height, fresh weight, etc., of G. sinensis increased to varying degrees compared with the salt-treated plants (Table 1). The relative water content of plants is a crucial measure of their water status, and water content in plant tissue has a direct impact on plant development and stomatal state, as well as on photosynthetic performance [40]. In this study, 200 mmol/L NaCl (S2) reduced its RWC by 11.69% compared to the control (Figure 1A).…”

Effect of some Pseudomonas strains and Agave americana L. on wheat germination under salt stress

“…In a similar study, reactive oxygen species (ROS) accumulation caused by tea polyphenols (TP) in wheat seedlings was shown to be accompanied by a decrease in REC (relative electrical conductivity), no change in MDA (molondialdehyde) content and an increase in Ca 2+ level. This suggests that TP treatment alone might provide a protective mechanism different from salt stress against ROS accumulation (64).…”

The Influence of EGCG on the Pharmacokinetics and Pharmacodynamics of Bisoprolol and a New Method for Simultaneous Determination of EGCG and Bisoprolol in Rat Plasma

“…Intake of catechins 400–500 mg daily can significantly reduce systolic and diastolic blood pressure ( 14 ). However, several studies have reported that green tea polyphenols may affect the expression or activities of drug-metabolizing enzymes such as CYP1A1, CYP2D6, CYP3A4 and drug transporters which leads to changes in the absorption and metabolism of certain drugs ( 15 , 16 ). In addition, it was reported that green tea catechins can inhibit the activities of CYP1A1, CYP2A6, CYP2C9 and CYP3A4 ( 17 ).…”