Effects of temperature and feed processing on protease activity and dietary protease on growths of white shrimp,Litopenaeus vannamei, and tilapia,Oreochromis niloticus × O. aureus

Abstract

Effects of temperature and processing on the stability of a commercial protease were assessed in an in vitro trial followed by growth trials with white shrimp and tilapia. Results showed that the protease has a high heat stability against pelleting, and it could hydrolyse dietary proteins during feed processing. In Exp. 2, white shrimp (3.3 g) fed low fish meal (FM) diet (LFD) with protease addition had similar growth to that fed high fish meal diet, and both had higher weight gain (WG) and lower feed conversion ratio (FCR) than that fed LFD without protease addition (P < 0.05). In Exp. 3, compressed (CD) or extruded (ED) diets containing 30 g kg À1 or 90 g kg À1 FM were supplemented with or without protease and then fed to tilapia (1.7 g) for 8 weeks. WG was improved and FCR decreased (P < 0.05) by the supplementation of protease in 30 g kg À1 FMCD, but not in 90 g kg À1 FMCD and ED diets when compared to those diets without protease supplementation. The digestibility trial with CD indicated the improved apparent digestibilities of dry matter and crude protein by dietary protease in 30 g kg À1 FMCD, but not in 90 g kg À1 FMCD. Results above showed that the protease has a high heat stability and the supplementation of protease in CD with low FM level can improve the growth of shrimp and tilapia.

Effects of dietary coated protease on growth performance, feed utilization, nutrient apparent digestibility, intestinal and hepatopancreas structure in juvenile Gibel carp (Carassius auratus gibelio )

“…SGR responses revealed the most consistent patterns, with reduced values in fish fed the NC diet without protease supplement, and with increasing values with incremental increases in dietary protease up to 300 mg/kg, and the FCR and PER were also improved in fish fed the diets with supplemented with protease. These findings further confirmed that the lower protein diet supplemented with protease reduced the dietary protein requirements, which were also reported in weaned piglet (Zuo et al., ) and white shrimp, Litopenaeus vannamei (Li et al., ). However, in our study, compared to the fish fed the dietary containing 300 mg/kg protease,the values of SGR in the fish fed the dietary containing 600 mg/kg protease declined slightly.…”

“…In rainbow trout, addition of protease to canola, pea‐based diets resulted in significant improvements in the coefficients of total tract apparent digestibility for crude protein, energy, lipid and dry matter (Drew et al., ). In tilapia, the digestibility of dry matter and crude protein with compressed diet increased by the supplementation of protease (Li et al., ). Our study also showed that digestibility of crude protein and crude lipid was significantly improved.…”

Growth response of African catfish, Clarias gariepinus (B.), larvae and fingerlings fed protease‐incorporated diets

“…Similarly, ADC of crude protein of African catfish fingerlings increased as dietary protease levels increased with maximum values at 1,000-1,250 unit/kg diet. In tilapia hybrid, the digestibility of dry matter and crude protein in low fish meal compressed diet was increased by dietary protease (Li et al, 2016). In Gibel carp juveniles, protease supplementation improved protein efficiency ratio and protein digestibility (Liu et al, 2018).…”

Exogenous enzymes as functional additives in finfish aquaculture

“…However, these substitutes are often inconsistent in quality, possess anti‐nutrients and have poor nutritional profiles, thus limiting their application in compounded aquaculture feed (Ai et al, ; Francis et al, ). In this regard, the use of exogenous feed proteases has been one of the most widely used strategies to improve nutrient utilization efficacy and reduce the feed cost in the fish farming industry (Drew et al, ; Li et al, ).…”