Abstract-The subject of this experimental report is the application of nano particles in petroleum refinement. Sulfur removal from petroleum using mixed bed which contains nano molybdenum oxide and nano cobalt oxide, each of them in 50 volume percentage, is considered in this study. The X factor is defined as quality index in sulfur removal process. The properties related to the process characterization which is defined as X factor and pressure drop, are evaluated experimentally. Catalyst diameter, bed diameter, bed height, temperature and pressure, superficial velocity is changed and the effect of these on the value of X factor is obtained to present the optimum operational and geometrical parameters. Also, the difference between mathematical and experimental data of X factor is reported as uncertainty. Cost evaluation is also presents due to operation pressure and temperature.