Effects of temperature and glucose concentration on the growth and respiration of fungal species isolated from a highly productive coastal upwelling ecosystem

Fuentes, Marcelo; Quiñones, Renato A.; Gutiérrez, Marcelo H.; Pantoja, Silvio

doi:10.1016/j.funeco.2014.09.006

Cited by 16 publications

(17 citation statements)

References 89 publications

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“…For instance, it has been shown that several fungal strains recovered from mangrove systems are capable of growing on wood under high-salt conditions (Arfi et al 2013 ). In our study, the only isolated fungal strain— Sarocladium strictum , previously known as Acremonium strictum (Summerbell et al 2011 )—is likely well adapted to saline environments, as it was previously isolated from a marine ecosystem (Fuentes et al 2015 ). Here it originated from a salt-marsh soil inoculum.…”

Section: Discussionmentioning

confidence: 63%

“…It is generally believed that fungi are ubiquitous and capable of occupying virtually every ecological niche as a result of their ability to degrade a suite of organic compounds such as complex biological polymers. They may also play roles in degrading lignocellulose in marine environments (Richards et al 2012 ), where the major factors affecting their diversities are salt concentration and temperature (Fuentes et al 2015 ). For instance, it has been shown that several fungal strains recovered from mangrove systems are capable of growing on wood under high-salt conditions (Arfi et al 2013 ).…”

Section: Discussionmentioning

confidence: 99%

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Halotolerant microbial consortia able to degrade highly recalcitrant plant biomass substrate

Cortes-Tolalpa

Norder

Elsas

et al. 2018

Appl Microbiol Biotechnol

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The microbial degradation of plant-derived compounds under salinity stress remains largely underexplored. The pretreatment of lignocellulose material, which is often needed to improve the production of lignocellulose monomers, leads to high salt levels, generating a saline environment that raises technical considerations that influence subsequent downstream processes. Here, we constructed halotolerant lignocellulose degrading microbial consortia by enriching a salt marsh soil microbiome on a recalcitrant carbon and energy source, i.e., wheat straw. The consortia were obtained after six cycles of growth on fresh substrate (adaptation phase), which was followed by four cycles on pre-digested (highly-recalcitrant) substrate (stabilization phase). The data indicated that typical salt-tolerant bacteria made up a large part of the selected consortia. These were “trained” to progressively perform better on fresh substrate, but a shift was observed when highly recalcitrant substrate was used. The most dominant bacteria in the consortia were Joostella marina, Flavobacterium beibuense, Algoriphagus ratkowskyi, Pseudomonas putida, and Halomonas meridiana. Interestingly, fungi were sparsely present and negatively affected by the change in the substrate composition. Sarocladium strictum was the single fungal strain recovered at the end of the adaptation phase, whereas it was deselected by the presence of recalcitrant substrate. Consortia selected in the latter substrate presented higher cellulose and lignin degradation than consortia selected on fresh substrate, indicating a specialization in transforming the recalcitrant regions of the substrate. Moreover, our results indicate that bacteria have a prime role in the degradation of recalcitrant lignocellulose under saline conditions, as compared to fungi. The final consortia constitute an interesting source of lignocellulolytic haloenzymes that can be used to increase the efficiency of the degradation process, while decreasing the associated costs.Electronic supplementary materialThe online version of this article (10.1007/s00253-017-8714-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Halotolerant microbial consortia able to degrade highly recalcitrant plant biomass substrate

Cortes-Tolalpa

Norder

Elsas

et al. 2018

Appl Microbiol Biotechnol

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“…Although the hyphae are the basic units of filamentous fungi, we believe that a colonylevel focus is useful because (i) the colony represents the most prevalent fungal body state because soon after an individual hypha forms, it branches profusely, then anastomoses and finally coordinates growth and flow of nutrients to form complex tissue (Moore et al, 2011); (ii) in many cases, fungal colonies can be distinguished from one another by observing them on the surface of substrates such as plant tissues, soil and litter layers, and wood, to name just a few. For example, colony biomass accumulated after a given amount of time represents the closest proxy of body size as it has been applied to other organisms (see Fuentes et al, 2015). In addition, we advocate scaling to other various measures of colony size such as 'colony hyphal length', 'colony extension rate' or 'hyphal branching', all of which represent valuable metrics to infer the ecology of fungi.…”

Section: Definition Of Allometry and The Scaling Of Metabolic Ratementioning

confidence: 99%

Applying allometric theory to fungi

2017

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“…These biochemical signatures, and an elemental composition indicative of a marine planktonic source, have potential applications for the assessment of fungal contribution to marine microbial biomass and organic matter reservoirs, and the cycling of carbon and nutrients. geochemical role in the ocean , Richards et al 2012, Burgaud et al 2013, Wang et al 2014, Fuentes et al 2015, Bochdansky et al 2016, Taylor & Cunliffe 2016, Tisthammer et al 2016, Wang et al 2017, Jephcott et al 2017, Grossart et al 2019). These findings support inclusion of fungi in models of the marine microbial loop and the oceanic carbon cycle (Gutiérrez et al 2011, Cunliffe et al 2017, Jephcott et al 2017), but critical gaps in our knowledge have hindered a complete understanding of the role of fungi in marine ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Biochemical fingerprints of marine fungi: implications for trophic and biogeochemical studies

Gutiérrez

Vera

Srain

et al. 2020

Aquat. Microb. Ecol.

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Fungi are ubiquitous in the marine environment, but their role in carbon and nitrogen cycling in the ocean, and in particular the quantitative significance of fungal biomass to ocean biogeochemistry, has not yet been assessed. Determination of the biochemical and stable isotope composition of marine fungi can provide a basis for identifying fungal patterns in relation to other microbes and detritus, and thus allow evaluation of their contribution to the transformation of marine organic matter. We characterized the biochemical composition of 13 fungal strains isolated from distinct marine environments in the eastern South Pacific Ocean off Chile. Proteins accounted for 3 to 21% of mycelial dry weight, with notably high levels of the essential amino acids histidine, threonine, valine, lysine and leucine, as well as polyunsaturated fatty acids, ergosterol, and phosphatidylcholine. Elemental composition and energetic content of these marine-derived fungi were within the range reported for bacteria, phytoplankton, zooplankton and other metazoans from aquatic environments, but a distinct pattern of lipids and proteins was identified in marine planktonic fungi. These biochemical signatures, and an elemental composition indicative of a marine planktonic source, have potential applications for the assessment of fungal contribution to marine microbial biomass and organic matter reservoirs, and the cycling of carbon and nutrients.

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Effects of temperature and glucose concentration on the growth and respiration of fungal species isolated from a highly productive coastal upwelling ecosystem

Cited by 16 publications

References 89 publications

Halotolerant microbial consortia able to degrade highly recalcitrant plant biomass substrate

Halotolerant microbial consortia able to degrade highly recalcitrant plant biomass substrate

Applying allometric theory to fungi

Biochemical fingerprints of marine fungi: implications for trophic and biogeochemical studies

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