Effects of temperature and water activity change on ecophysiology of ochratoxigenic Aspergillus carbonarius in field-simulating conditions

Cervini, Carla; Gallo, Antonia; Piemontese, Luca; Magistà, Donato; Logrieco, Antonio F.; Ferrara, Massimo; Solfrizzo, Michele; Perrone, Giancarlo

doi:10.1016/j.ijfoodmicro.2019.108420

Cited by 23 publications

(21 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequent studies including drought stress showed that this influenced FB 1 production and actually stimulated contamination of ripening maize cobs, partially due to physiological effects on the maize plant [ 32 ]. Studies of ochratoxigenic species such as A. carbonarius , A. westerdijkiae , A. steynii and P. verrucosum on different matrices have also examined effects of CC-related abiotic factors on growth and toxin contamination [ 18 , 21 , 33 , 34 ]. Overall, the growth rates of the Aspergillus section Circumdati and Nigri species was not affected by CC-related interacting abiotic factors.…”

Section: Biodiversity Ecology and Resilience Of Toxigenic Fungi Imentioning

confidence: 99%

“…However, in vitro experiments, both on coffee-based media and on stored coffee beans, suggested that there was a stimulation of OTA production (e.g., A. westerdijkiae ), while for other species (e.g., A. carbonarius ) there was a reduction in toxin contamination [ 18 ]. Studies by Cervini et al with strains of A. carbonarius isolated from grapes showed that while growth was relatively unaffected on grape-based media by CC-related interacting factors (+4 °C, 400 vs. 1000 ppm CO 2 , water stress), toxin production was stimulated for some strains, and this was confirmed with an increase in relative gene expression of cluster genes involved in toxin biosynthesis [ 21 ].…”

Section: Biodiversity Ecology and Resilience Of Toxigenic Fungi Imentioning

confidence: 99%

“…In A. carbonarius , several ecophysiological studies simulating field conditions revealed that fungal growth and OTA production were determined by environmental factors such as temperature, water availability and photoperiod [ 21 , 48 , 49 ]. Additionally, in a recent study by Cervini et al, the effect of two different day/night temperature cycles (15–28 °C vs. 18–34 °C, 11.5 h/12.5 h dark/light), and two CO 2 concentrations (400 vs. 1000 ppm) were evaluated [ 20 ].…”

Section: Molecular Characterization and Genomic Evidence On Toxigementioning

confidence: 99%

“…New and emerging combinations of mycotoxins in food/feed represent the ability of fungi to adapt to such changing conditions [ 15 , 16 ]. In this respect, the effect of three-way interactions amongst environmental factors (temperature × water availability × CO 2 ) on the ecophysiology of toxigenic fungi and mycotoxin accumulation has been recently investigated by several authors [ 10 , 17 , 18 , 19 , 20 , 21 ]. Nevertheless, obtaining a clear picture of the influence of climate on overall crop contamination by mycotoxins and on toxigenic fungal ecology and adaptation is complex and frequently intractable; so the real effects of CC are difficult to ascertain.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Toxigenic Fungi and Mycotoxins in a Climate Change Scenario: Ecology, Genomics, Distribution, Prediction and Prevention of the Risk

et al. 2020

Self Cite

View full text Add to dashboard Cite

Toxigenic fungi and mycotoxins are very common in food crops, with noticeable differences in their host specificity in terms of pathogenicity and toxin contamination. In addition, such crops may be infected with mixtures of mycotoxigenic fungi, resulting in multi-mycotoxin contamination. Climate represents the key factor in driving the fungal community structure and mycotoxin contamination levels pre- and post-harvest. Thus, there is significant interest in understanding the impact of interacting climate change-related abiotic factors (especially increased temperature, elevated CO2 and extremes in water availability) on the relative risks of mycotoxin contamination and impacts on food safety and security. We have thus examined the available information from the last decade on relative risks of mycotoxin contamination under future climate change scenarios and identified the gaps in knowledge. This has included the available scientific information on the ecology, genomics, distribution of toxigenic fungi and intervention strategies for mycotoxin control worldwide. In addition, some suggestions for prediction and prevention of mycotoxin risks are summarized together with future perspectives and research needs for a better understanding of the impacts of climate change scenarios.

show abstract

Section: Biodiversity Ecology and Resilience Of Toxigenic Fungi Imentioning

confidence: 99%

Section: Biodiversity Ecology and Resilience Of Toxigenic Fungi Imentioning

confidence: 99%

Section: Molecular Characterization and Genomic Evidence On Toxigementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toxigenic Fungi and Mycotoxins in a Climate Change Scenario: Ecology, Genomics, Distribution, Prediction and Prevention of the Risk

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…They are mostly associated with dried and stored foods, especially cereal and coffee. Black Aspergilli , mainly A. carbonarius , are considered as the primary source of OTA on grapes and similar fruits produced on the berries during the growing season, mainly from maturing to ripening in sunlight at high temperatures [ 6 , 7 , 8 ]. In the traditional meat products with long maturity periods, especially in fermented meat products, toxigenic moulds are mainly comprised of Aspergillus and Penicillium spp., which often overgrow [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Influence of NaCl and Glucose Content on Growth and Ochratoxin A Production by Aspergillus ochraceus, Aspergillus carbonarius and Penicillium nordicum

Wang

Yan

Neng

et al. 2020

Toxins

View full text Add to dashboard Cite

Ochratoxin A (OTA) is a nephrotoxic mycotoxin, which deserves particular attention for its widespread contamination of a variety of food and feed. Aspergillus ochraceus, Aspergillus carbonarius, and Penicillium nordicum are an important source of OTA in three different kinds of food commodities, including cereals, grape and dried fruit products, and dry-cured meat products. Deeper knowledge of OTA production and mycelium growth related to the high-sugar or NaCl-rich environments was gained in this manuscript. A. ochraceus and P. nordicum were likely to have greater growth rates in medium supplied with certain concentrations of NaCl (0–80 g/L), and the colony diameter was the largest at the salt content of 40 g/L. P. nordicum was more suitable to grow in NaCl-riched medium, the OTA production was increased to 316 ppb from 77 ppb when 20 g/L NaCl was added. The capability of OTA production was inhibited when salt content was 40 g/L and 60 g/L in A. ochraceus and P. nordicum, respectively. As the glucose content increased to 250 g/L, the capacity of mycelium growth and sporulation was increased significantly in A. ochraceus and A. carbonarius. A. carbonarius was more suitable to grow in high-sugar grape products. OTA production was significantly promoted with an added 100 g/L glucose in A. carbonarius. OTA production was inhibited when glucose content was 150 g/L and in 200 g/L in A. ochraceus and A. carbonarius, respectively. NaCl and glucose have an effect on fungal growth and OTA production, and the activation of biosynthetic genes of OtaA. These results would allow designing new strategies to prevent OTA accumulation on sugar or NaCl-riched foodstuffs and achieve the objective to manufacture cereals, dried vine fruits and dry-cured ham, free of OTA.

show abstract

First evidence on the occurrence of multi-mycotoxins and dietary risk exposure to AFB1 along the cassava value chain in Uganda

Oyesigye,

Cervini,

Oluwakayode

et al. 2024

Mycotoxin Res

View full text Add to dashboard Cite

This study investigated the occurrence and distribution of multiple mycotoxins (aflatoxin B1, B2, G1, G2, fumonisins B1, B2, ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), and citrinin (CIT)) in cassava products and as assessed the potential risk of aflatoxin B1 (AFB1) exposure among cassava consumers. A total of 192 samples of cassava products (96 flour and 96 chips, each with 48 samples from farmer and 48 from wholesaler) were analysed using LC/MS–MS. All positive samples irrespective of their origin (flour or chips) exhibited AFB1 levels exceeding the EU regulatory threshold of 5 µg/kg. The sum of fumonisins (FB1 + FB2), ZEN, and DON were significantly (P < 0.05) higher in cassava flour (14.3 µg/kg; 3.71 µg/kg; 25.1 µg/kg) compared to chips (6.54 µg/kg; 1.25 µg/kg; 0.25 µg/kg), respectively. Aflatoxins G2 was not detected in any of 192 samples. Cassava flour samples from farmers exhibited significantly (P < 0.05) higher mean concentrations of AFB1 (27.1 µg/kg), total aflatoxins (78.2 µg/kg), and ochratoxin A (79.6 µg/kg) in contrast to wholesalers, whose mean levels were notably lower at 8.91, 5.79 µg/kg, and 2.44 µg/kg, respectively, pointing the likely critical source of mycotoxin contamination. Cassava consumers in Northern Uganda are at a higher risk, with an estimated 2.06 cancer cases per 100,000 individuals per year compared to those in Eastern Uganda at 0.25. This study underscores the urgent need for interventions to manage aflatoxins in cassava flour, particularly at farm level in Northern Uganda. It accentuates a shift market to household-level sampling and the need for analytical methods targeting multiple mycotoxins.

show abstract

Effects of temperature and water activity change on ecophysiology of ochratoxigenic Aspergillus carbonarius in field-simulating conditions

Cited by 23 publications

References 41 publications

Toxigenic Fungi and Mycotoxins in a Climate Change Scenario: Ecology, Genomics, Distribution, Prediction and Prevention of the Risk

Toxigenic Fungi and Mycotoxins in a Climate Change Scenario: Ecology, Genomics, Distribution, Prediction and Prevention of the Risk

The Influence of NaCl and Glucose Content on Growth and Ochratoxin A Production by Aspergillus ochraceus, Aspergillus carbonarius and Penicillium nordicum

First evidence on the occurrence of multi-mycotoxins and dietary risk exposure to AFB1 along the cassava value chain in Uganda

Contact Info

Product

Resources

About