Effects of temperature change on physiological and biochemical activities of 
            <i>Haliotis discus hannai</i> Ino

Jiang, Weiwei; Fang, Jianguang; Li, Jiaqi; Jiang, Zengjie; Mao, Yuze; Du, Meirong; Gao, Zhenkun; Chen, Qionglin

doi:10.3724/sp.j.1118.2017.16133

Cited by 7 publications

(4 citation statements)

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“…Temperature is one of the most important environmental factors that affect aquatic animals' physiological activities (Matoo et al, 2013). Research has shown that drastic changes in water temperature can induce changes in the physiology and biochemistry of aquatic invertebrates such as Crassostrea virginica (Matoo et al, 2013) and Haliotis discus hannai (Jiang et al, 2017). In this study, we measured enzyme activity as physicochemical indices in the gill tissue of individuals of Mizuhopecten yessoensis under high temperature fluctuation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the Mizuhopecten yessoensis gills under high temperature fluctuations

Liu

Tian

et al. 2023

Invertebrate Biology

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Temperature is one of the environmental factors affecting the physiological activities of aquatic animals. This study explored the gene expression and regulation mechanism in the gill tissues of the scallop Mizuhopecten yessoensis under the stress of high temperature fluctuations. We designed a high temperature fluctuation experiment, in which the water temperature was raised from 20 C to 23 C and 26 C and then decreased from 26 C to 23 C and 20 C, with a rate of heating and cooling of 0.5 C/ h. The experiment consisted of four cycles and lasted for 7.5 days. When the target temperature was reached and the next temperature increase or decrease began, the gills of scallops were collected to measure immune enzyme activities and for transcriptome analysis. Immunological results showed significant differences in enzyme activities of catalase, superoxide dismutase, total antioxidant capacity, and lysozyme in scallop gills at 20 C on the first day and 26 C on the fifth day. Therefore, we analyzed gene expression from gill samples from these two time points using transcriptomics. We referred to samples from these time points as the normal temperature group (NT) and high temperature group (HT). Transcriptome results indicated that 347 differentially expressed genes (DEGs) were found in HT versus NT. Through gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, we found that these DEGs were mainly involved in metabolic pathways and protein synthesis pathways and had significant effects on oxidative stress, apoptosis, body metabolism, and protein folding in M. yessoensis. We selected 62 DEGs related to heat shock, immunity, and metabolism, including 47 upregulated and 15 downregulated DEGs. In a subset of these genes, quantitative real-time PCR (qRT-PCR) analysis showed similar expression (R 2 = 0.81), thus validating the transcriptome data. Our results provide a theoretical basis for further analysis of the response mechanism in M. yessoensis to high temperature stress and for the development of molecular breeding technology for high temperature tolerance.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the Mizuhopecten yessoensis gills under high temperature fluctuations

Liu

Tian

et al. 2023

Invertebrate Biology

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“…Temperature is one of the main causes of oxidative stress in shellfish. Studies of Patinopecten yessoensis [12], Haliotis discus hannai Ino [39], Sinonovacula constricta [40], Crassadoma gigantea [41], Scapharca broughtonii [42], and other shellfish have confirmed that temperature can directly affect their antioxidant systems. GST is a key antioxidant enzyme involved in the clearance of reactive oxygen species (ROS), ligand transport, and regulating stress-mediated cell signaling pathways [43].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons

Zhang,

Tian

et al. 2023

Fishes

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Neptunea cumingii is an economically important marine shellfish found in the Yellow and Bohai Seas areas of China. In this study, samples of Neptunea cumingii were collected in Zhangzidao and Yantai during spring, summer, autumn, and winter to clarify the gene expression patterns and regulatory mechanisms in their gills in different seasons. Transcriptome analysis was conducted using Neptunea cumingii gill tissues, and genes with significantly different expression levels were extracted for functional verification. The most genes with differences in expression (DEGs) were found in comparisons of the winter and summer samples. Gene enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes terms showed that these DEGs were mainly involved in immune and metabolic pathways, and they had significant effects on oxidative stress, body metabolism, and protein synthesis in Neptunea cumingii. Further screening of DEGs identified thirty-four genes related to temperature regulation, comprising thirteen genes with roles in innate immunity in shellfish, twelve genes related to oxidative stress, and nine genes related to protein synthesis and energy metabolism. Eleven DEGs were randomly selected for qPCR verification, and the results were consistent with the transcriptome analysis results. In summary, the transcriptome results differed significantly between seasons in the gill tissues of Neptunea cumingii. The expression levels of immune regulatory genes could be promoted in Neptunea cumingii during the high temperature season, whereas the expression of these genes may be inhibited in the low temperature season. The results obtained in this study provide insights into the molecular defense mechanisms that might allow Neptunea cumingii to adapt to climate change.

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“…However, too rapid a drop in temperature can cause the body to produce large amounts of Reactive Oxygen Species (ROS), which can cause damage to cells, and irreversible damage to physiological functions and immune defenses, thus affecting the quality of their survival [2]. ROS were found in the scallop (Patinopecten yessoensis) [3], the wrinkled disc abalone (Haliotis discus Hannai Ino) [4], the razor clam (Sinonovacula constricta) [5], the rock scallop (Crassadoma gigantea) [6], and the quahog (Scapharca broughtonii) [7] and other shellfish have demonstrated that the oxidative system and physiological metabolism are highly related to temperature stress. Therefore, in this study, different cold stress-induced dormancy methods were applied to purified Ru shan oysters to explore the changes in basic nutrients, energy metabolism, and oxidative stress during the ecological ice temperature preservation process of oysters, and to provide technological and theoretical references for optimizing the preservation of bivalve shellfish for storage and improving the quality of their survival [8].…”

Section: Introductionmentioning

confidence: 99%

Effects of Cold Stress Mode on Nutrient Composition, Energy Metabolism and Oxidative Stress During the Ecological Ice-temperature Retention Period of the Milky Oyster, Crassostrea Gigas

Liu,

Zhu,

Liu

et al. 2024

IJFSA

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Objective: To investigate the effects of pre-cooling methods of anhydrous preservation and temperature stress on their oxidative immune system, and energy substance metabolism, and to select a better method of low-temperature stress. Methods: The oysters were preserved alive by three ice temperature-induced dormancy methods, namely, acute cooling, bulk ice cooling, and gradient cooling, respectively, and the degree of damage of different cold stress methods on the milky oyster was investigated by the basic nutrient composition, metabolic status, and oxidative stress indexes. Results: The survival rate, crude protein, and crude fat of oysters preserved by gradient cooling for 10d were higher than those of the acute cooling group and bulk ice cooling group, and the moisture content was not much different, and the bulk ice cooling group was slightly higher than the other two groups. The antioxidant enzyme activity and TBA content of oysters in all groups increased throughout the storage period, and the ice-cooling group was significantly higher than the other two groups (P<0.05); the glycogen consumption of the gradient cooling group was significantly lower than that of the acute and ice-cooling groups (P<0.05), and the lactic acid content of oysters in all groups increased, and the gradient cooling group was significantly lower than that of the acute and ice-cooling groups (P<0.05). Conclusion: The gradient cooling method induced oysters to enter the ecological ice temperature dormant state and then began to live without water, oysters in this method of antioxidant enzyme activity and lipid peroxidation have been reduced, the survival time of the oyster is longer, the energy metabolism of the material consumed less, and it is more conducive to the oyster's long time without water to survive.

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Effects of temperature change on physiological and biochemical activities of Haliotis discus hannai Ino

Cited by 7 publications

References 0 publications

Transcriptome analysis of the Mizuhopecten yessoensis gills under high temperature fluctuations

Transcriptome analysis of the Mizuhopecten yessoensis gills under high temperature fluctuations

Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons

Effects of Cold Stress Mode on Nutrient Composition, Energy Metabolism and Oxidative Stress During the Ecological Ice-temperature Retention Period of the Milky Oyster, Crassostrea Gigas

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