Effects of temperature on baseline and genotoxicant-induced DNA damage in haemocytes of Dreissena polymorpha

Buschini, Annamaria; Carboni, Pamela; Martino, Anna; Poli, Paola; Rossi, Carlo

doi:10.1016/s1383-5718(03)00050-0

Cited by 117 publications

(45 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The variation in DNA migration detected by the Comet assay shows a strong seasonal influence both in terms of baseline level and sensitivity towards the treatments, in agreement with our previous studies in aquatic organisms [53]. The primary DNA damage, before the induction of the repair system, is clearly visible after 3 h exposure as an increase in DNA migration in the blood samples collected from the fish populations maintained in water treated with NaClO or ClO 2 in October 2000 and in February 2001.…”

Section: Discussionsupporting

confidence: 91%

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Buschini

Martino

Gustavino

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

114

View full text Add to dashboard Cite

The detection of a possible genotoxic effect of surface water treated with disinfectants for potabilization is the aim of the present work. The Comet assay and the micronucleus test were applied in circulating erythrocytes of Cyprinus carpio. Young specimens (20-30 g) were exposed in experimental basins, built within the potabilization plant of Castiglione del Lago (Perugia, Italy). In this plant the water of the Trasimeno Lake is treated and disinfected for potabilization before it is distributed to the people in the net of drinkable water. A continuous flow of water at a constant rate was supplied to basins; the water was continuously treated at a constant concentration with one of the three tested disinfectants (sodium hypochlorite, peracetic acid and chloride dioxide), one control basin being supplied with untreated water. Three sampling campaigns were performed:

show abstract

Section: Discussionsupporting

confidence: 91%

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Buschini

Martino

Gustavino

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

114

View full text Add to dashboard Cite

show abstract

“…Although rather common in marine bivalves, there has been very little comparative research on thermal stress for freshwater mussels. Furthermore, what is available has focused on acute, laboratorycontrolled thermal challenges at extreme temperatures Luo et al, 2014;Buschini et al, 2003;Pandolfo et al, 2010). Therefore, the main objective of this study was to compare the response of a hypothesized thermally tolerant species (V. lienosa) with a thermally sensitive species (V. nebulosa) of unionid freshwater mussel to both an acute, laboratory-controlled thermal challenge and a field chronic warming simulation at environmentally relevant temperatures.…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological, biochemical, and molecular thermal stress response profiles for two Unionid freshwater mussel species

Payton

Johnson

Jenny

2016

Journal of Experimental Biology

View full text Add to dashboard Cite

Freshwater mussels, aquatic keystone species, are in global decline. Long life spans, sedentary lifestyles, and unique reproductive strategies involving obligate parasitic stages make unionid freshwater mussels particularly sensitive to environmental perturbations resulting from global climate change. A greater understanding of the mechanisms by which closely related species differ in their response to thermal challenge is critical for successful conservation and management practices. As such, both an acute heat shock and a chronic warming simulation were conducted in order to evaluate responses between hypothesized thermally tolerant (Villosa lienosa) and thermally sensitive (Villosa nebulosa) freshwater mussels in response to predicted thermal warming. Multiple biological responses were quantified, including mortality, condition index, growth rates, glycogen and triglyceride content, and candidate gene expression. During acute heat shock, both species upregulated HSP90 and HSP70, although V. lienosa showed consistently greater transcript levels during upregulation. This pattern was consistent during the chronic warming simulation, with V. nebulosa showing greater induction of HSP60. Chronic warming stimulated increases in condition index for V. nebulosa; however, declines in growth rates during a recovery period were observed with no concurrent change in tissue glycogen levels. This contrasts with V. lienosa, where tissue glycogen significantly increased during chronic warming, although no response was observed for condition index or growth rates. These biological differences might indicate disparate thermal stress response mechanisms correlated with metabolic demands and resource utilization, and could thus be a factor influencing current ranges of these two species and their ability to cope with future persistent warming in their native habitats.

show abstract

“…Our results indicated that the exposure of C. parallelus to sublethal concentrations of Mn 2+ , combined with the increase in water temperature, induced significantly higher DNA damage in the temperature treatments than in the control; thus, these results indicated the genotoxic potential of these experimental conditions. Earlier studies, which also used the comet assay method, have associated thermal stress with genotoxic effects in aquatic organisms, such as freshwater fish (Carassius auratus), crayfish (Astacus leptodactylus) and mussels (Dreissena polymorpha) (Anitha et al, 2000;Buschini et al, 2003;Malev et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures

et al. 2017

View full text Add to dashboard Cite

This study evaluates the effects of exposure to manganese (Mn 2+ ) for 96 hours at two different temperatures (24 and 27°C) on juveniles of Centropomus parallelus through the activities of glutathione S-transferase (GST) and catalase (CAT), micronuclei test (MN) and comet assay. The GST activity did not show any significant difference between the groups exposed to Mn 2+ and the respective control groups; in contrast, a major increase in the CAT activity was observed at 27°C in the group exposed to Mn 2+ compared to the control group. The genotoxic analyses showed that in all animals exposed to Mn 2+, the number of red cells with micronuclei increased significantly compared to the respective control groups. There was also a significant increase in the incidence of DNA damage in the groups exposed to Mn 2+. At a temperature of 24ºC, animals exposed to Mn 2+ had more DNA damage than those at 27°C. It is likely that the increase in temperature can also induce oxidative stress. Thus, we conclude that manganese is toxic to the fat snook juveniles, causing genotoxic damage, and when associated with an increase in temperature, manganese can also provoke an increase in oxidative stress.Keywords: Biomarkers, DNA damage, Enzymes, Genotoxicity, Metal, Micronuclei.Este estudo avaliou os efeitos da exposição ao manganês (Mn 2+ ), após 96 horas, a duas temperaturas (24 e 27°C) em juvenis de Centropomus parallelus por meio de análises bioquímicas (atividade das enzimas glutationa S-transferase (GST) e catalase (CAT)) e genotóxicas (teste do micronúcleo e ensaio cometa). A atividade da GST não mostrou diferença significativa entre os grupos expostos ao Mn 2+ e os seus respectivos grupos controle, enquanto que um aumento significativo na atividade da CAT foi observado a 27°C no grupo exposto ao Mn 2+ , quando comparado ao grupo controle. As análises genotóxicas mostraram que os animais expostos ao Mn 2+ tiveram aumento significativo na quantidade de células com micronúcleo em relação aos seus grupos de controles. Houve também aumento significativo na incidência de danos ao DNA nos grupos expostos a esse contaminante. Na temperatura de 24°C, os animais expostos ao Mn 2+ tiveram maior quantidade de danos no DNA em relação a 27°C. É provável que o aumento da temperatura também possa induzir o estresse oxidativo. Assim, concluímos que o manganês é tóxico para os juvenis de robalo, causando dano genotóxico, e quando associado a um aumento da temperatura, também pode provocar um aumento no estresse oxidativo.

show abstract

Effects of temperature on baseline and genotoxicant-induced DNA damage in haemocytes of Dreissena polymorpha

Cited by 117 publications

References 47 publications

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization

Comparative physiological, biochemical, and molecular thermal stress response profiles for two Unionid freshwater mussel species

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures

Contact Info

Product

Resources

About