Effects of Temperature on Haemocyte and Granulocyte Counts and Expressions of Immunity-Related Genes in Haemocytes of Scallop Chlamys farreri After Vibrio anguillarum Infection

Chen, Zhengqiang; Zhang, Dongdong; Xing, Jing; Zhan, Wenbin

doi:10.1007/s11802-019-4180-3

Cited by 4 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were found in the freshwater mussel S. cumingii, with total haemocyte count increase, especially the proportion of granulocytes, after bacterial infection (146). On the contrary, granulocyte count decreased in the scallop A. farreri when challenged with bacteria; such decline increased at high temperature (306). Regarding metazoan pathogens, it has been suggested that the bivalve immune system responds to trematode invasion by recruiting granulocytes (305).…”

Section: Biotic Stress: Pathogens and Harmful Algal Bloomssupporting

confidence: 63%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

View full text Add to dashboard Cite

Bivalve molluscs stand out for their ecological success and their key role in the functioning of aquatic ecosystems, while also constituting a very valuable commercial resource. Both ecological success and production of bivalves depend on their effective immune defence function, in which haemocytes play a central role acting as both the undertaker of the cellular immunity and supplier of the humoral immunity. Bivalves have different types of haemocytes, which perform different functions. Hence, identification of cell subpopulations and their functional characterisation in immune responses is essential to fully understand the immune system in bivalves. Nowadays, there is not a unified nomenclature that applies to all bivalves. Characterisation of bivalve haemocyte subpopulations is often combined with 1) other multiple parameter assays to determine differences between cell types in immune-related physiological activities, such as phagocytosis, oxidative stress and apoptosis; and 2) immune response to different stressors such as pathogens, temperature, acidification and pollution. This review summarises the major and most recent findings in classification and functional characterisation of the main haemocyte types of bivalve molluscs.

show abstract

Section: Biotic Stress: Pathogens and Harmful Algal Bloomssupporting

confidence: 63%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…C. farreri is one of the most important commercially farmed shellfish; in north China in 2019, the annual yield of scallops exceeded 1.83 million tons, providing a major contribution to national mariculture production [ 20 , 21 ]. Previous research has mainly fo-cused on the effects of high temperature on C. farreri at a molecular level [ 22 , 23 ]. The mac-roscopic description, from a metabolomics perspective, is still absent, but this may pro-vide powerful information to understand the response mechanism of C. farreri under heat stress.…”

Section: Introductionmentioning

confidence: 99%

Metabonomic Analysis Provides New Insights into the Response of Zhikong Scallop (Chlamys farreri) to Heat Stress by Improving Energy Metabolism and Antioxidant Capacity

et al. 2022

View full text Add to dashboard Cite

Temperature is an important factor affecting the growth, development and survival of marine organisms. A short episode of high temperature has been proven to be a severe threat to sustainable shellfish culture. Zhikong scallop (Chlamys farreri), a shellfish with broad economic and biological value in North China, has frequently experienced heat stress in summer in recent years. To understand the effects of heat stress on shellfish, the metabolism of C. farreri was analyzed after exposure to 27 °C for either 6 h or 30 d. After 6 h of heat stress exposure, a total of 326 and 264 significantly different metabolites (SDMs) were identified in gill and mantle tissues, respectively. After 30 d of heat stress exposure, a total of 381 and 341 SDMs were found in the gill and mantle tissues, respectively. These SDMs were mainly related to the metabolism of amino acids, carbohydrates, lipids and nucleotides. A decline in pyruvic acid, and an increase in citric acid and fumaric acid in the gills and mantle of C. farreri indicated an alteration in energy metabolism, which may be attributed to increased ATP production in order to overcome the heat stress. Among the SDMs, 33 metabolites, including pyruvic acid, glycine and citric acid, were selected as potential biomarkers for heat stress response in C. farreri. In addition, a decline in glutamine and β-Alanine levels indicated oxidative stress in C. farreri exposed to heat, as well as an increase in the total antioxidant capacity (T-AOC). Our findings suggested C. farreri have the potential to adapt to heat stress by regulating energy metabolism and antioxidant capacity.

show abstract