Effects of temperature on N2O production in the process of nitrogen removal by micro-expansion aerobic granular sludge

Chen, Lili; Wen, Zhidan; Liang, Hong; Gao, Dawen

doi:10.1080/19443994.2016.1187089

Cited by 8 publications

(9 citation statements)

References 15 publications

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“…Similarly, the temperature dependence of bioreactor performances is also reported (Watanabe et al, 2017), with lower observed N 2 O consumption activity at a low temperature (Wang et al, 2018). Although the temperature effect on N 2 O emissions has been investigated at the bioreactor level (Adouani et al, 2015; Chen et al, 2016), an in‐depth analysis of N 2 O consumption activity in pure cultures has not been reported. Our results demonstrate that the effect of temperature on N 2 O consumption activity is N 2 ORB species‐dependent (Figure 3 and Table S2), supporting the significance of enriching and leveraging efficient N 2 ORB as an N 2 O sink.…”

Section: Discussionmentioning

confidence: 99%

Temperature and oxygen level determine N₂O respiration activities of heterotrophic N₂O‐reducing bacteria: Biokinetic study

Zhou

Suenaga

Chang

et al. 2020

Biotech & Bioengineering

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Nitrous oxide (N2O), a potent greenhouse gas, is reduced to N2 gas by N2O‐reducing bacteria (N2ORB), a process which represents an N2O sink in natural and engineered ecosystems. The N2O sink activity by N2ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores the effects of temperature and oxygen exposure on biokinetics of pure culture N2ORB. Four N2ORB, representing either clade I type nosZ (Pseudomonas stutzeri JCM5965 and Paracoccus denitrificans NBRC102528) or clade II type nosZ (Azospira sp. strains I09 and I13), were individually tested. The higher activation energy for N2O by Azospira sp. strain I13 (114.0 ± 22.6 kJ mol−1) compared with the other tested N2ORB (38.3–60.1 kJ mol−1) indicates that N2ORB can adapt to different temperatures. The O2 inhibition constants (KI) of Azospira sp. strain I09 and Ps. stutzeri JCM5965 increased from 0.06 ± 0.05 and 0.05 ± 0.02 μmol L−1 to 0.92 ± 0.24 and 0.84 ± 0.31 μmol L−1, respectively, as the temperature increased from 15°C to 35°C, while that of Azospira sp. strain I13 was temperature‐independent (p = 0.106). Within the range of temperatures examined, Azospira sp. strain I13 had a faster recovery after O2 exposure compared with Azospira sp. strain I09 and Ps. stutzeri JCM5965 (p < 0.05). These results suggest that temperature and O2 exposure result in the growth of ecophysiologically distinct N2ORB as N2O sinks. This knowledge can help develop a suitable N2O mitigation strategy according to the physiologies of the predominant N2ORB.

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Section: Discussionmentioning

confidence: 99%

Temperature and oxygen level determine N₂O respiration activities of heterotrophic N₂O‐reducing bacteria: Biokinetic study

Zhou

Suenaga

Chang

et al. 2020

Biotech & Bioengineering

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“…N 2 O production during biological nitrogen removal was composed of gaseous N 2 O and dissolved N 2 O. The concentration of N 2 O was analyzed using a gas chromatograph (GC) equipped with an electron-capture detector (ECD) (GC-14B, Shimadzu, Japan) at 345°C [19] . Dissolved N 2 O was sampled and measured according to the study conducted by Chen et al [19] .…”

Section: Methodsmentioning

confidence: 99%

“…The concentration of N 2 O was analyzed using a gas chromatograph (GC) equipped with an electron-capture detector (ECD) (GC-14B, Shimadzu, Japan) at 345°C [19] . Dissolved N 2 O was sampled and measured according to the study conducted by Chen et al [19] . The analysis of N 2 O production was conducted in triplicate, and the average value was calculated.…”

Section: Methodsmentioning

confidence: 99%

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Effects of carbon source on N<sub>2</sub>O production in the process of simultaneous nitrification and denitrifica-tion via nitrite by aerobic granular sludge

Liang¹,

Li²,

Wang³

et al. 2016

Appl Env Biotechnol

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A sequencing batch reactor (SBR) was used to study the effect of carbon source (C6H12O6 and CH3COONa) and C/N ratio (C/N=4:1 and C/N=7:1) on the production of nitrous oxide (N2O) in the process of simultaneous nitrifica-tion and denitrification via nitrite (short-cut SND) by aerobic granular sludge and the removal efficiency of nitrogen under low dissolved oxygen (DO). The results showed that short-cut SND occurred in this system, and the removal ef-ficiency of total nitrogen (TN) at C6H12O6 and CH3COONa were 28.93 % and 41.19 %, respectively. However, the production of N2O significantly increased when CH3COONa was used as a carbon source. In addition, the rate of N2O release when CH3COONa was a carbon source was 8.34 times the rate when C6H12O6 was the carbon source. With the increase of C/N, removal rate of TN and the efficiency of the short-cut SND were increased. The removal efficiency of TN at C/N=7:1 was 90.33%, which was 2.19 times at C/N=4:1. The percentage of short-cut SND at C/N=4:1 and C/N=7:1 were 87.47% and 95.97%, respectively. The release rate of N2O from the original 1.14 mg/(g • min) decreased to 0.10 mg/(g • min) after increased the C/N from 4:1 to 7:1.

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“…On the other hand, NO is another detrimental byproduct which is toxic for the activities of microbes and has harmful effects on the ozone layer (Wang et al, 2016). Both N 2 O and NO are mainly produced and emitted during nitrification and can have a considerable effect on the carbon footprint from wastewater treatment systems (Sun et al, 2017;Spinelli et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The effect of temperature shifts on N2O and NO emissions from a partial nitritation reactor treating reject wastewater

et al. 2018

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Temperature has a known effect on ammonia oxidizing bacteria (AOB) activities, reducing its ammonia oxidizing rate (AOR) when temperature is lowered. However, little is known concerning its effect on NO and NO emissions which are produced during ammonia oxidation having a greenhouse effect. To study this, an AOB enriched partial nitrification sequencing batch reactor (PN-SBR) was operated within a two step-wise feed under 5 different temperatures (30-25-20-15-10 °C). A decrease on the specific AOR (sAOR) was detected when decreasing the temperature. NO emissions were also affected by the temperature but only the ones produced during the first aeration of the cycle, when AOBs shifted from a period of low activity to a period of high activity. NO emission factors (%) detected during the second aerobic phase were similar among all temperatures tested and lower than the emissions detected during the first aerated phase. The average NO emission factor was in the range of 0.15-0.70% NO-N/NH-N oxidized in the first aeration phase and 0.14-0.15% NO-N/NH-N-oxidized in the second aeration phase at 10 to 30 °C, respectively. On the other hand, NO emissions were very similar under all temperatures resulting in 0.03-0.06% of NH-N oxidized.

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Effects of temperature on N2O production in the process of nitrogen removal by micro-expansion aerobic granular sludge

Cited by 8 publications

References 15 publications

Temperature and oxygen level determine N₂O respiration activities of heterotrophic N₂O‐reducing bacteria: Biokinetic study

Temperature and oxygen level determine N₂O respiration activities of heterotrophic N₂O‐reducing bacteria: Biokinetic study

Effects of carbon source on N<sub>2</sub>O production in the process of simultaneous nitrification and denitrifica-tion via nitrite by aerobic granular sludge

The effect of temperature shifts on N2O and NO emissions from a partial nitritation reactor treating reject wastewater

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Effects of temperature on N2O production in the process of nitrogen removal by micro-expansion aerobic granular sludge

Cited by 8 publications

References 15 publications

Temperature and oxygen level determine N2O respiration activities of heterotrophic N2O‐reducing bacteria: Biokinetic study

Temperature and oxygen level determine N2O respiration activities of heterotrophic N2O‐reducing bacteria: Biokinetic study

Effects of carbon source on N<sub>2</sub>O production in the process of simultaneous nitrification and denitrifica-tion via nitrite by aerobic granular sludge

The effect of temperature shifts on N2O and NO emissions from a partial nitritation reactor treating reject wastewater

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Temperature and oxygen level determine N₂O respiration activities of heterotrophic N₂O‐reducing bacteria: Biokinetic study

Temperature and oxygen level determine N₂O respiration activities of heterotrophic N₂O‐reducing bacteria: Biokinetic study