Effects of ten-week 30% caloric restriction on metabolic health and skeletal muscles of adult and old C57BL/6J mice

Kvedaras, Mindaugas; Minderis, Petras; Krusnauskas, Raulas; Ratkevičius, Aivaras

doi:10.1016/j.mad.2020.111320

Cited by 11 publications

(3 citation statements)

References 46 publications

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“…Succinate dehydrogenase (SDH) activity. We used the method to assess SDH activity described previously 54,55 . Briefly, 10 mg of skeletal muscle (m. gastrocnemius or m. soleus) tissue was homogenised in 100 μL ice cold lysis buffer (50 mM Tris-HCl, 1 mM ethylene diamine tetra acetic acid, 1 mM ethylene bis (oxyethylene nitrilo) tetra acetic acid, 50 mM sodium fluoride, 1 mM sodium orthovanadate, 10 mM β-glycerophosphate, 1% Triton X-100, pH adjusted to 7.0).…”

Section: Denervationmentioning

confidence: 99%

Methionine restriction plus overload improves skeletal muscle and metabolic health in old mice on a high fat diet

Swaminathan

Fokin

Venckūnas

et al. 2021

Sci Rep

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Methionine restriction (MR) has been shown to reduce the age-induced inflammation. We examined the effect of MR (0.17% methionine, 10% kCal fat) and MR + high fat diet (HFD) (0.17% methionine, 45% kCal fat) on body mass, food intake, glucose tolerance, resting energy expenditure, hind limb muscle mass, denervation-induced atrophy and overload-induced hypertrophy in young and old mice. In old mice, MR and MR + HFD induced a decrease in body mass. Muscle mass per body mass was lower in old compared to young mice. MR restored some of the HFD-induced reduction in muscle oxidative capacity. The denervation-induced atrophy of the m. gastrocnemius was larger in animals on MR than on a control diet, irrespective of age. Old mice on MR had larger hypertrophy of m. plantaris. Irrespective of age, MR and MR + HFD had better glucose tolerance compared to the other groups. Young and old mice on MR + HFD had a higher resting VO2 per body mass than HFD group. Mice on MR and MR + HFD had a resting respiratory quotient closer to 0.70, irrespective of age, indicating an increased utilization of lipids. In conclusion, MR in combination with resistance training may improve skeletal muscle and metabolic health in old age even in the face of obesity.

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Section: Denervationmentioning

confidence: 99%

Methionine restriction plus overload improves skeletal muscle and metabolic health in old mice on a high fat diet

Swaminathan

Fokin

Venckūnas

et al. 2021

Sci Rep

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“…CR exerts a protective effect against sarcopenia in both rodents and non-human primates ( 32 – 35 ). A CR of 30% over 10 weeks may improve skeletal muscle function in male C57BL/6 mice ( 33 ). Lifelong 8% CR prevents age-related disruption of the myofiber membrane environment in male Fischer-344 rats ( 32 ).…”

Section: Cr and Frailtymentioning

confidence: 99%

Caloric Restriction May Help Delay the Onset of Frailty and Support Frailty Management

Liu

2021

Front. Nutr.

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Frailty is an age-related clinical syndrome that may increase the risk of falls, disability, hospitalization, and death in older adults. Delaying the progression of frailty helps improve the quality of life in older adults. Caloric restriction (CR) may extend lifespan and reduce the risk of age-related diseases. However, few studies have explored the relationship between CR and frailty. In this review, we focused on the impact of CR on frailty and aimed to identify potential associated mechanisms. Although CR may help prevent frailty, further studies are required to determine the underlying mechanisms and specific CR regimens suitable for use in humans.

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“…Our previous study showed that weight and fat loss does not depend on macronutrient proportions of the diet in adult mice undergoing CR [11]. It is important to investigate if this is true for old mice, which show greater weight loss than adult mice when subjected to 30% CR [15]. There is evidence that a high-protein diet might be effective in reducing the body fat increase during the refeeding after CR [16,17].…”

Section: Introductionmentioning

confidence: 99%

Effects of Diet Macronutrient Composition on Weight Loss during Caloric Restriction and Subsequent Weight Regain during Refeeding in Aging Mice

Minderis,

Fokin,

Povilonis

et al. 2023

Nutrients

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Caloric restriction (CR) induces weight loss, but is associated with rapid weight regain upon return to ad libitum feeding. Our aim was to investigate effects of the macronutrient composition of the diet on weight loss and regain in elderly mice. Males, 18 months old, of the C57BL/6J strain were subjected to 4-week 30% CR followed by 4 weeks of ad libitum refeeding on either high-carb (HC), high-fat (HF) or high-protein (HP) diets (n = 22 each). Mice (n = 11) fed a chow diet ad libitum served as a control group (CON). Body mass and food intake were monitored daily. Twenty-four-hour indirect calorimetry was used to assess energy expenditure and substrate oxidation. Muscle and fat mass were evaluated with dissection of the tissues. Serum leptin and ghrelin levels were also measured. CR-induced weight loss did not differ between the diets. Weight regain was particularly fast for HF as mice overshot their initial weight by 12.8 ± 5.7% after 4-week refeeding when HC and HP mice reached the weight of the CON group. Weight regain strongly correlated with energy intake across the groups. The respiratory exchange ratio was lower in HF mice (0.81 ± 0.03) compared to HC (0.94 ± 0.06, p < 0.001), HP (0.89 ± 0.04, p < 0.001) and CON mice (0.91 ± 0.06, p < 0.01) during the refeeding. Serum leptin levels were higher in HF mice (1.03 ± 0.50 ng/mL) compared to HC (0.46 ± 0.14, p < 0.001), HP (0.63 ± 0.28, p < 0.05) or CON mice (0.41 ± 0.14, p < 0.001). Thus, CR induces similar weight loss in aging mice irrespective of the diet’s macronutrient composition. An HF diet leads to excessive energy intake and pronounced gain in body fat in spite of increased fat oxidation and serum leptin during the refeeding after CR.

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Effects of ten-week 30% caloric restriction on metabolic health and skeletal muscles of adult and old C57BL/6J mice

Cited by 11 publications

References 46 publications

Methionine restriction plus overload improves skeletal muscle and metabolic health in old mice on a high fat diet

Methionine restriction plus overload improves skeletal muscle and metabolic health in old mice on a high fat diet

Caloric Restriction May Help Delay the Onset of Frailty and Support Frailty Management

Effects of Diet Macronutrient Composition on Weight Loss during Caloric Restriction and Subsequent Weight Regain during Refeeding in Aging Mice

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