Effects of Testicular and Non-Testicular Testosterone on Territorial and Isolation-induced Aggressive Behavior of Male Layer Chicks

Abstract

Testosterone (T) is known to induce aggressive behavior, particularly in male animals. However, our recent results showed that a certain kind of aggressive behavior is T-independent; moreover, the role of T in chicken territorial and isolation-induced aggressive behavior has not yet been investigated. In addition, castration alone is insufficient to evaluate the role of T in aggressive behavior because we found that non-testicular T concentration, probably derived from the adrenal gland, in the blood of castrated chicks was low, but not zero. In the present study, therefore, the role of testicular T in chicken aggressive behavior was evaluated through castration, and the role of non-testicular T was assessed using the subcutaneous implantation of flutamide, a non-steroidal antiandrogen, in the castrated male layer chicks. Resident-intruder (R-I) and social interaction (SI) tests were used to quantitatively monitor territorial and isolation-induced aggressive behavior, respectively. Castration and drug implantation of the chicks were performed at 14 days of age. The R-I test was performed at 29 and 30 days of age, and the SI test was performed at 31 and 32 days of age. The total aggression frequencies (TAFs) and aggression establishment rate (AER) were used as indices of chick aggressive behavior. In the R-I test, castration significantly decreased the TAFs but the AER was not affected by castration or flutamide implantation. In the SI test, on the other hand, there were no significant differences in the TAFs, but the AER tended to increase in the intact chicks and decrease in the flutamide-implanted, castrated male chicks. These results suggest that the role of T in chicken aggression depends on the differences in social context of the behavior, and that both testicular and non-testicular T play an important role in the occurrence of isolation-induced aggression in male layer chicks.