Effects of the Addition of Poly(ethylene Glycol) and Non-ionic Surfactants on Pretreatment, Enzymatic Hydrolysis, and Ethanol Fermentation

Nogueira, Cleitiane da Costa; Padilha, Carlos Eduardo de Araújo; Filho, Pedro F. Souza; Santos, Everaldo Silvino dos

doi:10.1007/s12155-021-10388-9

Cited by 20 publications

(2 citation statements)

References 122 publications

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“…The comparatively smaller conversion found for the delignified leaves compared to the filter paper and pretreated sawdust can be attributed, therefore, to the occlusion of cellulose fibers inside the vegetal cell wall and tissue structure. The surfactant effect would involve both the enhanced adsorption/desorption of enzyme from the reaction sites and the separation and exposure of cellulose fibers to the enzyme. , However, the effect of nonionic surfactants on saccharification of the tested LCB pretreated with HPAC was minor due to residual lignin or to the attainment of an already open and accessible cellulose structure.…”

Section: Resultsmentioning

confidence: 99%

Cellulose Enzymatic Saccharification Intensified by Nonionic Surfactants

Balbi

Fleite

Kildegaard

et al. 2023

Ind. Eng. Chem. Res.

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Enzymatic saccharification of cellulose is one of the bottlenecks of second-generation bioethanol production and other bioprocesses aimed at the use of lignocellulosic residues. Hence, it is crucial to find conditions and additives that can improve this process. In this work, the use of different nonionic surfactants was studied. The results demonstrate that all tested additives increase the initial rate of filter paper saccharification, with PEG 600 more than doubling the initial rate for two cellulose enzymatic cocktails. The highest effects were found with surfactants of linear structure, which likely better separate the filter paper cellulose fibers, allowing enzyme access. The effect of surfactants was larger for experiments without agitation. However, orbital agitation improved both the initial rate and yields. The effect of the gas–liquid interfacial area was negligible. Despite the beneficial trends found for the filter paper, no significant improvements were evidenced while adding surfactants to the saccharification of acid-oxidative pretreated biomass. Similarly, carboxymethyl cellulose saccharification was only marginally enhanced. Hence, the substrate structure and delignification method have a strong effect on the influence of surfactant addition and should be considered for deciding the saccharification media formulation.

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Section: Resultsmentioning

confidence: 99%

Cellulose Enzymatic Saccharification Intensified by Nonionic Surfactants

Balbi

Fleite

Kildegaard

et al. 2023

Ind. Eng. Chem. Res.

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“…Several studies propose the use of molecules with surfactant properties to improve the enzymatic hydrolysis of lignocellulosic materials [ 29 ]. For example, a study aimed at developing an optimized model for low-cost enzymatic hydrolysis of hydrothermal-alkaline/oxygen pre-treated reed showed that it was possible to reduce the enzymatic load from 20 to 3 FPU/g of pre-treated biomass by adding 0.03 g/g-PS of polyethylene glycol (PEG) 3000 to the hydrolysis carried out with 2% ( w / v ) of solids content [ 30 ].…”

Section: Additives As Emerging Tools To Enhance Enzymatic Hydrolysismentioning

confidence: 99%

Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass

et al. 2022

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Lignocellulosic biomass (LCB) has remained a latent alternative resource to be the main substitute for oil and its derivatives in a biorefinery concept. However, its complex structure and the underdeveloped technologies for its large-scale processing keep it in a state of constant study trying to establish a consolidated process. In intensive processes, enzymes have been shown to be important molecules for the fractionation and conversion of LCB into biofuels and high-value-added molecules. However, operational challenges must be overcome before enzyme technology can be the main resource for obtaining second-generation sugars. The use of additives is shown to be a suitable strategy to improve the saccharification process. This review describes the mechanisms, roles, and effects of using additives, such as surfactants, biosurfactants, and non-catalytic proteins, separately and integrated into the enzymatic hydrolysis process of lignocellulosic biomass. In doing so, it provides a technical background in which operational biomass processing hurdles such as solids and enzymatic loadings, pretreatment burdens, and the unproductive adsorption phenomenon can be addressed.

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Distinct effects of dilute acid prehydrolysate inhibitors on enzymatic hydrolysis and yeast fermentation

Tan,

Wen,

et al. 2024

Bioprocess Biosyst Eng

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Effects of the Addition of Poly(ethylene Glycol) and Non-ionic Surfactants on Pretreatment, Enzymatic Hydrolysis, and Ethanol Fermentation

Cited by 20 publications

References 122 publications

Cellulose Enzymatic Saccharification Intensified by Nonionic Surfactants

Cellulose Enzymatic Saccharification Intensified by Nonionic Surfactants

Surfactants, Biosurfactants, and Non-Catalytic Proteins as Key Molecules to Enhance Enzymatic Hydrolysis of Lignocellulosic Biomass

Distinct effects of dilute acid prehydrolysate inhibitors on enzymatic hydrolysis and yeast fermentation

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