Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model

Rodríguez-Sorrento, Agustina; Castillejos, Lorena; López-Colom, Paola; Cifuentes-Orjuela, Gloria; Rodrı́guez-Palmero, Marı́a; Muñoz, José Antonio Moreno; Luise, Diana; Trevisi, Paolo; Martín‐Orúe, S. M.

doi:10.3389/fmicb.2021.642549

Cited by 14 publications

(12 citation statements)

References 87 publications

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“…infantis CECT 7210—has proven to be effective in reducing Salmonella loads in piglets in previous works ( Barba-Vidal et al, 2017 ). Interestingly, the former strain administered together with L. rhamnosus HN001 was also capable of diminishing Salmonella and ETEC F4 presence as shown by our research group in the past ( Rodríguez-Sorrento et al, 2020 , 2021 ). Nevertheless, in the present trial, combining this probiotic with inulin and FOS was unable to reduce Salmonella excretion, suggesting that the combination of this Bifidobacterium strain with these prebiotics does not improve its power to fight the pathogen.…”

Section: Discussionsupporting

confidence: 55%

Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model

et al. 2022

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The use of bifidobacteria as probiotics has proven to be beneficial in gastroenteric infections. Furthermore, prebiotics such as inulin can enhance the survival and growth of these bacteria. Two trials were performed to evaluate the effects of the administration of Bifidobacterium longum subsp. infantis CECT 7210 and oligofructose-enriched inulin against Salmonella enterica serovar Typhimurium or enterotoxigenic Escherichia coli (ETEC) F4. A total of 72 (Salmonella trial) and 96 (ETEC F4 trial) weaned piglets were used in a 2 × 2 design (with or without synbiotic, inoculated or not with the pathogen). After adaptation, animals were orally inoculated. Performance and clinical signs were evaluated. On days 4 and 8 (Salmonella trial) and 3 and 7 (ETEC F4 trial) post-inoculation (PI), one animal per pen was euthanized. Blood, digestive content and tissue samples were collected and microbiological counts, fermentation products, serum inflammatory markers and ileum histomorphometry analysis were performed. Both challenges had an impact on faecal consistency (p < 0.001), including the faecal shedding of Salmonella and increased numbers of enterobacteria and coliforms. The synbiotic administration did not have any effect on pathogen loads but induced changes in the fermentation profile, such as increased valeric acid in both trials as well as decreased acetic acid, except for Salmonella-challenged animals. The effect on propionate varied among trials, increasing in challenged synbiotic-treated pigs and decreasing in non-challenged ones in the Salmonella trial (P interaction = 0.013), while the opposed occurred in the ETEC F4 trial (P interaction = 0.013). The administration of the synbiotic increased intraepithelial lymphocytes (IEL; p = 0.039) on day 8 PI in the Salmonella trial and a similar trend occurred in non-challenged pigs in the ETEC F4 trial (P interaction = 0.086). The results did not provide evidence of reduced pathogen load with the synbiotic, although a modulation in fermentative activity could be identified depending on the challenge. Consistent increases were found in IEL, suggesting that this synbiotic combination has some immunomodulatory properties.

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Section: Discussionsupporting

confidence: 55%

Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model

et al. 2022

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“…Barba-Vidal et al and Rodrigues-Sorrento et al reported a reduction in colonic ammonia levels after supplementation with Bifidobacterium longum subsp. infantis in piglets challenged with ETEC K88 [189] or ETEC F4 [190] Meanwhile, probiotic feeding in pigs reduces skatole concentrations, as observed in multiple studies [177,180,191]. Similarly, supplementation of either Bacillus subtilis DSM 32315 with xylooligosaccharides (XOS) or a combination of Lactobacillus plantarum B90 and Saccharomyces cerevisiae P11 decreased indole concentrations [176,180].…”

Section: Influence Of Probiotics Supplementation On Mi-crobial Metabo...mentioning

confidence: 84%

Gut microbiome-produced metabolites in pigs: a review on their biological functions and the influence of probiotics

Vasquez¹,

Oh²,

Song³

et al. 2022

J Anim Sci Technol

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The gastrointestinal tract is a complex ecosystem that contains a large number of microorganisms with different metabolic capacities. Modulation of the gut microbiome can improve the growth and promote health in pigs. Crosstalk between the host, diet, and the gut microbiome can influence the health of the host, potentially through the production of several metabolites with various functions. Short-chain and branched-chain fatty acids, secondary bile acids, polyamines, indoles, and phenolic compounds are metabolites produced by the gut microbiome. The gut microbiome can also produce neurotransmitters (such as γ-aminobutyric acid, catecholamines, and serotonin), their precursors, and vitamins. Several studies in pigs have demonstrated the importance of the gut microbiome and its metabolites in improving growth performance and feed efficiency, alleviating stress, and providing protection from pathogens. The use of probiotics is one of the strategies employed to target the gut microbiome of pigs. Promising results have been published on the use of probiotics in optimizing pig production. This review focuses on the role of gut microbiome-derived metabolites in the performance of pigs and the effects of probiotics on altering the levels of these metabolites.

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“…For the trial, the enterotoxigenic Escherichia coli (ETEC) F4 strain COLI30/14-3 used (positive for virulence factors F4ab, F4ac, LT, STb, and EAST1 and negative for K99, F6, F18, F41, STa, VT1, VT2, and EAE) was isolated from feces of a 14-week-old pig from a farm with a clinical course of colibacillosis and provided by the Veterinary Laboratory of Diagnosis of Infectious Diseases of UAB. This strain was actually used with the same purpose in previous studies of our group [22]. The oral inoculums were prepared by an overnight incubation at 37 • C in Brain Heart Infusion (BHI) with slow agitation (1× g) in an orbital incubator.…”

Section: Bacterial Strainmentioning

confidence: 99%

Effectiveness of Two Plant-Based In-Feed Additives against an Escherichia coli F4 Oral Challenge in Weaned Piglets

Montoya

D’Angelo

Martín‐Orúe

et al. 2021

Animals

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This study evaluates the efficacy of two plant-based feed supplementations to fight colibacillosis in weanlings. A total of 96 piglets (32 pens) were assigned to four diets: a control diet (T1) or supplemented with ZnO (2500 ppm Zn) (T2) or two different plant supplements, T3 (1 kg/t; based on essential oils) and T4 (T3 + 1.5 kg/t based on non-volatile compounds). After one week, animals were challenged with ETEC F4, and 8 days after, one animal per pen was euthanized. Performance, clinical signs, microbial analysis, inflammatory response, intestinal morphology, and ileal gene expression were assessed. ZnO improved daily gains 4 days after challenge, T3 and T4 showing intermediate values (96, 249, 170, and 157 g/d for T1, T2, T3, and T4, p = 0.035). Fecal lactobacilli were higher with T3 and T4 compared to ZnO (7.55, 6.26, 8.71, and 8.27 cfu/gFM; p = 0.0007) and T3 increased the lactobacilli/coliforms ratio (p = 0.002). T4 was associated with lower levels of Pig-MAP (p = 0.07) and increases in villus/crypt ratio (1.49, 1.90, 1.73, and 1.84; p = 0.009). Moreover, T4 was associated with an upregulation of the REG3G gene (p = 0.013; pFDR = 0.228) involved in the immune response induced by enteric pathogens. In conclusion, both plant supplements enhanced animal response in front of an ETEC F4 challenge probably based on different modes of action.

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Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model

Cited by 14 publications

References 87 publications

Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model

Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model

Gut microbiome-produced metabolites in pigs: a review on their biological functions and the influence of probiotics

Effectiveness of Two Plant-Based In-Feed Additives against an Escherichia coli F4 Oral Challenge in Weaned Piglets

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