Effects of the Autoxidized Oils on Lipid Metabolism and Drug Metabolizing Enzyme System in Rats

Arakaki, Mitsuo; Ariyoshi, Toshihiko

doi:10.3358/shokueishi.18.474

Cited by 2 publications

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“…Figure 4 shows that the cytochrome P-450 content in rat liver microsomes increased at first (induction period) and then decreased (reduction period) by the consecutive oral dose of AL for 15 days. Now we can better explain the incompatible phenomena reported previously (5,6) for the changes in cytochrome P-450 content by repeated AL administration. The increase and the decrease of cytochrome P-450 content by repeated AL administration previously reported probably corresponds to observations in the induction and reduction periods, respectively.…”

Section: Discussionmentioning

confidence: 66%

Effect of autoxidized linoleic acid on the contents of cytochrome P-450 and cytochrome b5, and drug-metabolizing enzyme activities in rat liver.

Hiramatsu¹,

Kishida²,

Natake³

1987

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryAutoxidized linoleic acid (AL) having 800meq/kg of per oxide value and 1,700meq/kg of carbonyl value was given in repeated oral doses for 1-15 days at a daily dose of 2.5ml/kg to male Wistar rats. During the administration, the effect of AL on drug-metabolizing activity was investigated periodically in liver microsomes. The contents of cyto chrome P-450 and b5 were increased by consecutive oral doses for 3-7 days. Thereafter, the amount of cytochrome P-450 decreased gradually, but the b5 decreased slightly. Thus, after administration for 11-15 days, the cytochrome P-450 content was significantly lower but the cytochrome b5 content was rather high in comparison with the control group. The aminopyrin-N-demethylase activity was not reduced even after repeated oral doses of AL for 15 days. The activation of 2-acetyl amino fluorene (2 AAF), which the authors termed S-9 activity, gradually decreased during administration for more than 3 days, and completely disappeared after administration for 9 days.

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Section: Discussionmentioning

confidence: 66%

Effect of autoxidized linoleic acid on the contents of cytochrome P-450 and cytochrome b5, and drug-metabolizing enzyme activities in rat liver.

Hiramatsu¹,

Kishida²,

Natake³

1987

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…There have been some incompatible findings on the effect of autoxidized oil on drug-metabolizing activity in rat liver microsomes under the respective experimental conditions (1,2). Therefore, in our previous work (3), AL having 800meq/kg of peroxide value and 1,700meq/kg of carbonyl value was administered periodically for 1-15 days at a daily dose of 2.5ml/kg body weight to male Wistar rats, and the effect of AL on the drug-metabolizing system in rat liver microsomes was studied.…”

mentioning

confidence: 99%

Effects of dose levels of autoxidized linoleic acid on the drug-metabolizing system in rat liver.

Hiramatsu¹,

Kishida²,

Natake

1988

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryAutoxidized linoleic acid (AL) having 800meq/kg of per oxide value and 1,700meq/kg of carbonyl value was given in repeated oral doses at a daily dose of 0 (control)-7.5ml/kg to male Wistar rats for 5 successive days. The effect of increasing AL dose on the drug-metabolizing system was investigated in rat liver microsomes and S-9 fractions. All the rats of a daily dose of 5.0-7.5ml/kg died after the third day of consecutive oral doses. The cytochrome P-450 and b5 contents, enzyme activities in electron transfer system, aminopyrin-N-demethylase activity and S-9 activity (metabolic activation of 2-acetylaminofluorene) in the drug metabolizing system changed essentially in a similar manner, that is, both the contents and the activities were increased by a small dose of AL, and were decreased by a large dose of AL. These results strongly supported the fi ndings in a previous report wherein we observed the periodical effect of AL dose on the drug-metabolizing system. Key Words autoxidized linoleic acid, microsome, cytochrome P-450, cytochrome b5, electron transfer system, drug-metabolizing activity, aminopyrin-N-demethylase, S-9 activity, membrane disorder There have been some incompatible findings on the effect of autoxidized oil on drug-metabolizing activity in rat liver microsomes under the respective experimental conditions (1, 2). Therefore, in our previous work (3), AL having 800meq/kg of peroxide value and 1,700meq/kg of carbonyl value was administered periodically for 1-15 days at a daily dose of 2.5ml/kg body weight to male Wistar rats, and the effect of AL on the drug-metabolizing system in rat liver microsomes was studied. The cytochrome P-450 and b5 contents were increased by consecutive oral doses of AL for 3-7 days, then the amount of cytochrome P-450 decreased gradually, but the b5 decreased slightly. Thus, after administration for 11-15 days, the cytochrome P -450 content was significantly lower, but the cytochrome b5 content was rather high 97

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Effects of the Autoxidized Oils on Lipid Metabolism and Drug Metabolizing Enzyme System in Rats

Cited by 2 publications

References 0 publications

Effect of autoxidized linoleic acid on the contents of cytochrome P-450 and cytochrome b5, and drug-metabolizing enzyme activities in rat liver.

Effect of autoxidized linoleic acid on the contents of cytochrome P-450 and cytochrome b5, and drug-metabolizing enzyme activities in rat liver.

Effects of dose levels of autoxidized linoleic acid on the drug-metabolizing system in rat liver.

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