Effects of the combination between selected phytochemicals and the carriers silica and Tween 80 on dry matter and neutral detergent fibre digestibility of common feeds

Simoni, Marica; Temmar, Rokia; Bignamini, Davide A.; Foskolos, Andreas; Sabbioni, A.; Ablondi, Michela; Quarantelli, A.; Righi, Federico

doi:10.1080/1828051x.2020.1787882

Cited by 4 publications

(3 citation statements)

References 37 publications

(44 reference statements)

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“…On each farm, individual rumen fluids were collected from a total of three Holstein cows on each diet type, by using an oesophageal probe as described by Cinotti (1987), 3 h after the morning feed delivery. The individual rumen fluids maintained at 39 C were mixed in the laboratory in anaerobic conditions described by Simoni et al (2020). Each rumen-fluid mix was blended, filtered through four layers of cheesecloth and divided into two aliquots.…”

Section: Methodsmentioning

confidence: 99%

Determination of the optimal priming interval of rumen fluids used as inocula for the in vitro digestibility trials through radial enzyme diffusion method

et al. 2021

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Context. Determination of the neutral detergent fibre digestibility is one of the important parameters to consider when formulating diets. However, the in vitro determination shows low repeatability because of the source of rumenfluid inoculum. Priming of the rumen fluid inocula, obtained through an oesophageal probe, has been proposed to overcome this issue. Aim. The objective of the study was to investigate the evolution of the microbial enzymatic activities of different rumen fluids during a priming procedure, to establish the fermentation interval that minimises the differences among rumen-fluid degradative potentials. Methods. Three farms for each type of diet were involved in the study. Rumen fluids were obtained from dry and lactating cows fed the following four diet types: 100% hay or a diet with 80 : 20 forage : concentrate ratio (F : C) as drycow diets, and ad libitum hay and concentrate, or a total mixed ration (both at 60 : 40 F : C) as lactating-cow diets. On each farm, rumen fluid was collected from three Holstein cows by using an oesophageal probe, and mixed. Two aliquots of each rumen fluid mix were added to the medium containing the same priming substrate in an in vitro batchfermentation system. During the incubation, the fermentation fluids were sampled in duplicate at 0-, 1-, 2-, 4-, 8-, 24-and 48-h intervals. Enzymatic activities of amylase, cellulase and xylanase were determined by radial enzyme diffusion method. Key results. Initial enzymatic activities were quite variable and increased with an increasing incubation time. By 24 h, amylase showed similar values among high-concentrate diet fermentation fluids, and a lower data dispersion in comparison to the other intervals; cellulase was characterised by similar values in all the fermentation fluids derived from diets including concentrates, and xylanase showed similar activity in the fermentation fluids derived from highconcentrate diets. Development of the enzymatic activity of the fermentation fluids derived from the 100% hay diet differed from the others. Conclusions. A 24-h priming procedure was needed to stabilise and equalise the enzymatic activity of the rumen fluid from cows fed high-concentrate diets. This was not observed in rumen fluid from cows fed hay-based diets. Implications. The 24-h-primed rumen fluid can be used to increase the repeatability of neutral detergent fibre digestibility determination.

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Section: Methodsmentioning

confidence: 99%

Determination of the optimal priming interval of rumen fluids used as inocula for the in vitro digestibility trials through radial enzyme diffusion method

et al. 2021

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“…The DM content was measured by drying the sample at 103 C overnight. The uNDF was determined through a 240 h in vitro fermentation using the procedure described in Righi et al (2017); the rumen fluid was collected at the slaughterhouse from the rumen of 4 cows and processed as described by Simoni, Temmar, et al (2020) and Simoni, Tsiplakou, et al (2020). The aNDF, ADF and ADL were analysed according to Van Soest (1994).…”

Section: Samples' Collection and Chemical Analysismentioning

confidence: 99%

The use of visible/near-infrared spectroscopy to predict fibre fractions, fibre-bound nitrogen and total-tract apparent nutrients digestibility in beef cattle diets and faeces

Simoni

Goi

Marchi

et al. 2021

Italian Journal of Animal Science

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“…Starter and milk were sampled weekly. Starter samples were processed as described by Righi et al (2017), Simoni et al (2020) and Comino et al (2014). In brief, dried samples were ground in a Cyclotec mill (Tecator, Herndon, VA) to pass a 1-mm screen and analysed for crude protein (CP) and ether extract (EE), according to AOAC (2005).…”

Section: Calf Starter and Milk Analysismentioning

confidence: 99%

Low doses of lactoferrin supplementation in weaning calves

Simoni

Baldrighi²,

Degola³

et al. 2020

Acta fytotechn zootechn

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Effects of the combination between selected phytochemicals and the carriers silica and Tween 80 on dry matter and neutral detergent fibre digestibility of common feeds

Abstract: Righi (2020) Effects of the combination between selected phytochemicals and the carriers silica and Tween 80 on dry matter and neutral detergent fibre digestibility of common feeds,

Cited by 4 publications

References 37 publications

Determination of the optimal priming interval of rumen fluids used as inocula for the in vitro digestibility trials through radial enzyme diffusion method

Determination of the optimal priming interval of rumen fluids used as inocula for the in vitro digestibility trials through radial enzyme diffusion method

The use of visible/near-infrared spectroscopy to predict fibre fractions, fibre-bound nitrogen and total-tract apparent nutrients digestibility in beef cattle diets and faeces

Low doses of lactoferrin supplementation in weaning calves

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