Effects of the Combined Application of Trimethylated Chitosan and Carbodiimide on the Biostability and Antibacterial Activity of Dentin Collagen Matrix

Abstract

The structural integrity of a dentin matrix that has been demineralized by the clinical use of etchants or calcium-depleting endodontic irrigants, such as endodontic ethylenediaminetetraacetic acid (EDTA), is often deteriorated due to the collagenolytic activities of reactivated endogenous enzymes as well as the infiltration of extrinsic bacteria. Therefore, the biomodification of dentin collagen with improved stability and antibacterial activity holds great promise in conservative dentistry. The purpose of this study was to evaluate the effects of the combined application of trimethylated chitosan (TMC) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) on the biostability and antibacterial activity of the demineralized dentin collagen matrix. The morphological changes in the collagen matrix were observed by scanning electron microscopy (SEM), the amount of TMC adsorbed on the collagen surface was detected by X-ray photoelectron spectroscopy, and the elastic modulus was measured by a three-point bending device. Dry weight loss and amino acid release were detected to evaluate its anti-collagenase degradation performance. The antibacterial performance was detected by confocal microscopy. The TMC-treated group had less collagen space and a more compact collagen arrangement, while the untreated group had a looser collagen arrangement. The combined application of TMC and EDC can increase the elastic modulus, reduce the loss of elastic modulus, and result in good antibacterial performance. The current study proved that a dentin collagen matrix biomodified by TMC and EDC showed improved biodegradation resistance and antibacterial activities.

Treated Dentin Matrix in Tissue Regeneration: Recent Advances

“…The molecules with or without capsule modifications can be detached to the surface or into the tubule chamber of TDM, which are later released from the substance. During research work on bio-root construction, immunological rejection caused by xenograft [ 30 , 31 , 66 , 83 ], oxidative stress injury and infection is an inevitably encountered challenge; it could be worth making attempts to load drug molecules of immune response regulators [ 30 , 31 ], antioxidants [ 29 , 32 ], antibiotics [ 84 ] and bio-active agents [ 28 , 34 ] for the purpose of achieving better bio-root regeneration.…”

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

“…In previous studies, chitosan and norfloxacin were physically added or hydrogen bonded, which were not stable enough. To solve the above limitations, a new hemostatic and antibacterial composite material was prepared by grafting chitosan, which has good biocompatibility, and NF, which has a significant antibacterial effect under aqueous phase conditions, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as catalysts via chemical modification [ 23 , 24 ]. Its hemostatic effect was evaluated by whole blood clotting index (BCI) measurement and coagulation in vitro and in vivo, so as to provide a theoretical basis for the application of CTS-NF composites in the pharmaceutical field in the future.…”