Effects of the Developmental Stage of Extract Donor Embryos on the Culture of Marine Medaka Oryzias dancena Embryonic Stem Cell-like Cells

Abstract

Optimizing the conditions for stem cell culture is an essential prerequisite for the efficient utilization of stem cells. In the culture of fish embryonic stem cells (ESCs) or ESC-like cells, embryo extracts are important for stable growth, but there is no rule for determining the developmental stage of the embryos used to obtain extracts. Therefore, this study investigated the effects of the developmental stage of extract donor embryos on the culture of Oryzias dancena ESC-like cells. O. dancena ESC-like cells were cultured in different media containing each of four types of embryo extract depending on the developmental stage of the extract donor embryos. Growth, morphology, colony-forming ability, alkaline phosphatase (AP) activity, and embryoid body (EB) formation of the cells were investigated. While the developmental stage of the extract donor embryos did not influence the growth, morphology, AP activity, or EB formation of ESC-like cells, colony-forming ability was affected and the pattern of the effects differed completely between the two ESC-like cells investigated. These results suggest that the developmental stage of extract donor embryos should be selected carefully for the culture of ESC-like cells, according to the research purpose and type of cell line.

A review of studies on Oryzias dancena

“…Salinity tolerance Inoue and Takei (2003) [ 7 ] Kang et al (2008) [ 8 ] Cho et al (2010a) [ 5 ] 6. Reproduction Song et al (2009a) [ 18 ] Song et al (2009b) [ 19 ] Cho et al (2010b) [ 20 ] Lim et al (2012) [ 21 ] Takehana et al (2014) [ 22 ] Im et al (2016) [ 23 ] Ryu and Gong (2017) [ 24 ] Park (2020) [ 25 ] 7. Fish disease Park et al (2019) [ 26 ] 8.…”

“…Optimizing the conditions for stem cell culture is an essential prerequisite for the efficient utilization of stem cells [ 24 ]. In the culture of fish embryonic stem cells (ESCs) or ESC-like cells, embryo extracts are important for stable growth, but there is no rule for determining the developmental stage of the embryos used to obtain extracts [ 24 ]. Therefore, Ryu and Gong [ 24 ] investigated the effects of the developmental stage of extract donor embryos on the culture of Oryzias dancena ESC-like cells.…”

“…In the culture of fish embryonic stem cells (ESCs) or ESC-like cells, embryo extracts are important for stable growth, but there is no rule for determining the developmental stage of the embryos used to obtain extracts [ 24 ]. Therefore, Ryu and Gong [ 24 ] investigated the effects of the developmental stage of extract donor embryos on the culture of Oryzias dancena ESC-like cells. O. dancena ESC-like cells were cultured in different media containing each of four types of embryo extract depending on the developmental stage of the extract donor embryos.…”

“…While the developmental stage of the extract donor embryos did not influence the growth, morphology, AP activity, or EB formation of ESC-like cells, colony-forming ability was affected and the pattern of the effects differed completely between the two ESC-like cells investigated. These results suggest that the developmental stage of extract donor embryos should be selected carefully for the culture of ESC-like cells, according to the research purpose and type of cell line [ 24 ].…”

“…The toxic effects of MB were clearly dose dependent for each life stage. The toxicity sensitivity of juveniles to MB was dramatically higher than that of other groups [ 24 ]. In 48 h after sterilization, cortisol and glucose concentrations of the adult group with MB treatment were higher than those of the adult group with no treatment.…”