Effects of the doubly labelled water procedure on Great Tits<i>Parus major</i>feeding young

Hinsley, Shelley A.; Bellamy, Paul E.; Rothery, P.; Redman, Paula; Furness, Lindsay; Speakman, John R.

doi:10.1080/00063657.2010.543647

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…We show significant elevation of isotopes after 60 min, the usual plateau sampling time for seabirds Welcker et al 2009Welcker et al , 2010. We suggest that future studies consider using a plateau of 90 min, although effects on subject behavior need to be considered Hinsley et al 2011). Isotope dilution is likely a useful method for determining body composition (percent lipids) in species where lipids are below 15% and SCM is no longer reliable.…”

Section: What Is the Best Methods For Measuring Body Energy Stores?mentioning

confidence: 87%

Determining Seabird Body Condition Using Nonlethal Measures

Jacobs

Elliott

Guigueno

et al. 2012

Physiological and Biochemical Zoology

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Energy stores are critical for successful breeding, and longitudinal studies require nonlethal methods to measure energy stores ("body condition"). Nonlethal techniques for measuring energy reserves are seldom verified independently. We compare body mass, size-corrected mass (SCM), plasma lipids, and isotopic dilution with extracted total body lipid content in three seabird species (thick-billed murres Uria lomvia, all four measures; northern fulmars Fulmarus glacialis, three measures; and black-legged kittiwakes Rissa tridactyla, two measures). SCM and body mass were better predictors of total body lipids for the species with high percent lipids (fulmars; R2 = 0.5-0.6) than for the species with low percent lipids (murres and kittiwakes; R2 = 0.2-0.4). The relationship between SCM and percent body lipids, which we argue is often a better measure of condition, was also poor (R2 < 0.2) for species with low lipids. In a literature comparison of 17 bird species, percent lipids was the only predictor of the strength of the relationship between mass and total body lipids; we suggest that SCM be used as an index of energy stores only when lipids exceed 15% of body mass. Across all three species we measured, SCM based on the ordinary least squares regression of mass on the first principal component outperformed other measures. Isotopic dilution was a better predictor of both total body lipids and percent body lipids than were mass, SCM, or plasma lipids in murres. Total body lipids decreased through the breeding season at both sites, while total and neutral plasma lipid concentrations increased at one site but not another, suggesting mobilization of lipid stores for breeding. A literature review showed substantial variation in the reliability of plasma markers, and we recommend isotopic dilution (oxygen-18, plateau) for determination of energy reserves in birds where lipid content is below 15%.

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Section: What Is the Best Methods For Measuring Body Energy Stores?mentioning

confidence: 87%

Determining Seabird Body Condition Using Nonlethal Measures

Jacobs

Elliott

Guigueno

et al. 2012

Physiological and Biochemical Zoology

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“…Given that extensive restraint alters birds' behaviour, and consequently daily energy expenditure (Schultner et al, 2010;Hinsley et al, 2011), we attempted a modified two-sample technique. To reduce disturbance and associated possibility of abandonment with a costly accelerometer attached, we injected the DLW intravenously and our second sample was taken only 12 min after injection to reduce the impact of extended restraint on behaviour.…”

Section: Doubly Labelled Watermentioning

confidence: 99%

“…Daily energy expenditure has seldom been reported in the order Suliformes (Shaffer, 2011; referred to there as 'Pelecaniformes' from which Suliformes has recently been split), only once in cormorants (Keller and Visser, 1999), and never in a small cormorant species, presumably because many cormorants are very sensitive to disturbance. The two-sample DLW method requires holding large animals for over an hour, and that lengthy period of restraint can cause abandonment of offspring or altered behaviour (Schultner et al, 2010;Hinsley et al, 2011). Obtaining direct estimations of daily energy expenditure would be useful for validating estimates from bioenergetic models used to quantify the impact of cormorants on fisheries (Ridgway, 2010).…”

Section: Introductionmentioning

confidence: 99%

Counting calories in cormorants: dynamic body acceleration predicts daily energy expenditure measured in pelagic cormorants

Stothart

Elliott

Wood

et al. 2016

Journal of Experimental Biology

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The integral of the dynamic component of acceleration over time has been proposed as a measure of energy expenditure in wild animals. We tested that idea by attaching accelerometers to the tails of freeranging pelagic cormorants (Phalacrocorax pelagicus) and simultaneously estimating energy expenditure using doubly labelled water. Two different formulations of dynamic body acceleration, [vectorial and overall DBA (VeDBA and ODBA)], correlated with mass-specific energy expenditure (both R 2 =0.91). VeDBA models combining and separately parameterizing flying, diving, activity on land and surface swimming were consistently considered more parsimonious than time budget models and showed less variability in model fit. Additionally, we observed evidence for the presence of hypometabolic processes (i.e. reduced heart rate and body temperature; shunting of blood away from non-essential organs) that suppressed metabolism in cormorants while diving, which was the most metabolically important activity. We concluded that a combination of VeDBA and physiological processes accurately measured energy expenditure for cormorants.

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“…The novel object was removed as soon as the observation was completed. All observations (one control and two neophobia tests) took place on the same day to control for provisioning differences depending on the age of the offspring, with an interval of 1 h between experimental trials, more than enough time for pairs to return fully to provisioning behaviour (see Hinsley et al 2011). All observations were carried out by the same observer (C. Colchester).…”

Section: Field Experimentsmentioning

confidence: 99%