Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells

Andrade, Deyse Valverde Gomes de; Oliveria, Diêgo Madureira de; Barreto, George E.; Bertolino, Laura-Aon; Saraceno, Ezequiel; Capani, Francisco; Giráldez, Lisandro

doi:10.1016/j.brainres.2008.01.006

Cited by 27 publications

(9 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The occurrence of polyploidy and polyembryony in other species of the genus Anemopaegma of probable hybrid origin (Sampaio 2010;Firetti-Leggieri et al 2011) suggest the existence of an agamic polyploid complex in this group, as postulated for some species of Handroanthus (Piazzano 1998;Costa et al 2004;Bittencourt & Semir 2005;Bittencourt & Moraes 2010). These results make this group an important subject for apomixis investigations, not only because of evolutionary and ecological implications, but also because of their high economic potential in pharmacology (Gentry 1992;Uchino et al 2004;De Andrade et al 2008), since apomictic and self-fertile species may facilitate genetic breeding programmes (Asker & Jerling 1992;Koltunow 1993;Hörandl 2010).…”

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

Mating in the pseudogamic apomicticAnemopaegma acutifoliumDC: another case of pseudo‐self‐compatibility inBignoniaceae?

2012

View full text Add to dashboard Cite

Self-compatibility in apomictic pseudogamic species is considered fundamental to assure reproduction by seeds in extreme situations, making apomictic species more advantageous than sexual ones in these scenarios. Anemopaegma acutifolium is a polyploidy, apomictic sporophytic species with no endosperm development in ovules of unpollinated pistils, which indicates obligate pseudogamy. Thus, the aim of the present work is to study the breeding system and post-pollination events to test if there is similar pseudogamous development irrespective of pollination treatment. We analysed fruit and seed set obtained in controlled experimental pollinations, as well as embryo number per seed, and the progress of ovule penetration, fertilisation and early endosperm development between self- and cross-pollinated pistils. We found that the species is self-fertile and that spontaneous selfing fruit set is also possible, although emasculated flowers never form fruits. Selfed pistils were as efficient as crossed ones for all parameters analysed, except for a delay in endosperm development observed in the former that may be an effect of the late-acting self-incompatibility. Therefore, the avoidance of selfed pistil abortion seems to be promoted by the presence of adventitious embryos and a normal endosperm. We conclude that A. acutifolium shows apomixis-related pseudo-self-compatibility, as in other self-fertile apomictic species of Bignoniaceae, which confer reproductive assurance and increases fruit-set and persistence ability in fast-changing tropical habitats.

show abstract

Section: Discussionmentioning

confidence: 88%

“…; De Andrade et al . ), since apomictic and self‐fertile species may facilitate genetic breeding programmes (Asker & Jerling ; Koltunow ; Hörandl ).…”

Section: Discussionmentioning

confidence: 99%

Mating in the pseudogamic apomicticAnemopaegma acutifoliumDC: another case of pseudo‐self‐compatibility inBignoniaceae?

2012

View full text Add to dashboard Cite

show abstract

“…MPP + and ROT are commonly used instead of PQ, because of their well-known mechanisms of toxicity. In addition, studies using MPP + and ROT have already given significant insights into the molecular mechanisms of dopaminergic neuronal death [24, 31]. However, there is a need for more reliable cultured cell models of PD.…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells

Martins

Bastos

Carvalho

et al. 2013

Journal of Toxicology

View full text Add to dashboard Cite

Paraquat (PQ), a cationic nonselective bipyridyl herbicide, has been used as neurotoxicant to modulate Parkinson's disease in laboratory settings. Other compounds like rotenone (ROT), a pesticide, and 1-methyl-4-phenylpyridinium ion (MPP+) have been widely used as neurotoxicants. We compared the toxicity of these three neurotoxicants using differentiated dopaminergic SH-SY5Y human cells, aiming to elucidate their differential effects. PQ-induced neurotoxicity was shown to be concentration and time dependent, being mitochondrial dysfunction followed by neuronal death. On the other hand, cells exposure to MPP+ induced mitochondrial dysfunction, but not cellular lyses. Meanwhile, ROT promoted both mitochondrial dysfunction and neuronal death, revealing a biphasic pattern. To further elucidate PQ neurotoxic mechanism, several protective agents were used. SH-SY5Y cells pretreatment with tiron (TIR) and 2-hydroxybenzoic acid sodium salt (NaSAL), both antioxidants, and N ω-nitro-L-arginine methyl ester hydrochloride (L-NAME), a nitric oxide synthase inhibitor, partially protected against PQ-induced cell injury. Additionally, 1-(2-[bis(4-fluorophenyl)methoxy]ethyl)-4-(3-phenyl-propyl)piperazine (GBR 12909), a dopamine transporter inhibitor, and cycloheximide (CHX), a protein synthesis inhibitor, also partially protected against PQ-induced cell injury. In conclusion, we demonstrated that PQ, MPP+, and ROT exerted differential toxic effects on dopaminergic cells. PQ neurotoxicity occurred through exacerbated oxidative stress, with involvement of uptake through the dopamine transporter and protein synthesis.

show abstract

“…Rotenone has been widely used in neurotoxic models as it causes molecular and cellular processes similar to those observed in neurodegenerative disease like PD (Cabezas et al 2012;de Oliveria et al 2009de Oliveria et al , 2011Greenamyre et al 2003;Valverde et al 2008). Rotenone has a direct effect on mitochondrial functions, including an interference with the electron transport chain, loss of mitochondrial membrane's potential, and ATP generation (Greenamyre et al 2003;Sarafian et al 2010;Simpkins et al 2010).…”

Section: Discussionmentioning

confidence: 96%

PDGF-BB Protects Mitochondria from Rotenone in T98G Cells

et al. 2014

View full text Add to dashboard Cite

Rotenone is one of the most-studied neurotoxic substances as it induces oxidative stress processes both in cellular and animal models. Rotenone affects ATP generation, reactive oxygen species (ROS) production, and mitochondrial membrane potential in neurons and astrocyte-like cells. Previous epidemiologic studies have supported the role of neurotrophic factors such as BDNF and GDNF in neuroprotection mainly in neurons; however, only very few studies have focused on the importance of astrocytic protection in neurodegenerative models. In the present study, we assessed the neuroprotective effects of PDGF-BB against toxicity induced by rotenone in the astrocytic-like model of T98G human glioblastoma cell line. Our results demonstrated that pretreatment with PDGF-BB for 24 h increased cell viability, preserved nuclear morphology and mitochondrial membrane potential following stimulation with rotenone, and reduced ROS production nearly to control conditions. These observations were accompanied by important morphological changes induced by rotenone and that PDGF-BB was able to preserve cellular morphology under this toxic stimuli. These findings indicated that PDGF-BB protects mitochondrial functions, and may serve as a potential therapeutic strategy in rotenone-induced oxidative damage in astrocytes.

show abstract

Effects of the extract of Anemopaegma mirandum (Catuaba) on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells

Cited by 27 publications

References 52 publications

Mating in the pseudogamic apomicticAnemopaegma acutifoliumDC: another case of pseudo‐self‐compatibility inBignoniaceae?

Mating in the pseudogamic apomicticAnemopaegma acutifoliumDC: another case of pseudo‐self‐compatibility inBignoniaceae?

Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells

PDGF-BB Protects Mitochondria from Rotenone in T98G Cells

Contact Info

Product

Resources

About