Effects of the fungicide prochloraz on the sexual development of zebrafish (Danio rerio)

Kinnberg, Karin Lund; Holbech, Henrik; Petersen, Gitte I.; Bjerregaard, Poul

doi:10.1016/j.cbpc.2006.11.002

Cited by 66 publications

(52 citation statements)

References 33 publications

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“…The sections were stained with hematoxylin-eosin, and examined with an Olympus CX31 light microscope (Olympus, Tokyo, Japan), focusing on sexual differentiation and histological abnormalities of the gonad (e.g., intersex). The gonads were classified as testes or ovaries, based on the presence of spermatogenetic cells or oocytes, respectively, according to Kinnberg et al (2007). Gonads where a single oocyte at the primary growth stage was observed in otherwise testicular tissue were still classified as testes.…”

Section: Histology and Sex Determinationmentioning

confidence: 99%

The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations

et al. 2015

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Section: Histology and Sex Determinationmentioning

confidence: 99%

The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations

et al. 2015

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“…Sex differentiation is also influenced by sex steroids and sex hormone mimicking or antagonizing substances. Changes in the proportions of males and females were observed in populations of juvenile zebrafish exposed, during the critical period of gonadal development, to 17α-ethinyloestradiol and methyltestosterone (Örn et al 2003), trenbolone (Örn et al 2006), anti-oestrogen ZM 189, 156 (Andersen et al 2004, the fungicide prochloraz (Kinnberg et al 2007), and bisphenol A . Recently, effects of the thyroid hormone inhibitor perchlorate on the sex ratio were also studied.…”

Section: Discussionmentioning

confidence: 99%

Influence of Propylparaben on Vitellogenesis and Sex Ratio in Juvenile Zebrafish (Danio rerio)

et al. 2009

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The aim of the study was to evaluate the xeno-oestrogenic potential of propylparaben in vivo using zebrafish (Danio rerio). Experimental juvenile zebrafish (20 days post hatching) were fed a feed containing 500, 1000, or 2000 mg·kg -1 of propylparaben, fish in a positive control group were given a feed treated with 20 mg·kg -1 of 17β-oestradiol, and the control fish were given the feed free of either tested substance. The exposure of fish to propylparaben did not affect vitellogenesis after 20 days exposure but seemed to influence the sex differentiation processes, as evidenced by a sex ratio significantly skewed towards females in the group fed 500 mg·kg -1 of propylparaben following 45 days of exposure. The potential of the fish to respond to oestrogenic stimulation was confirmed, since significantly higher vitellogenin concentrations were detected in the fish from the positive control group.

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“…However, in the male fish, significantly increased VTG concentrations were observed in the groups exposed to 16 or 64 mg/L prochloraz. The authors postulate that this is attributable to the ability of prochloraz to target multiple steroidogenic enzymes, thus leading to different imbalances in the estrogen and androgen hormone levels at different exposure concentrations [47].…”

Section: Compilation Of Toxicity Data For Fadrozole and Prochlorazmentioning

confidence: 99%

“…Zebrafish were exposed to prochloraz (0, 16, 64, or 202 mg/ L) for 60 d from 24 h postfertilization [47]. Fish groups exposed to 202 mg/L prochloraz showed an increased proportion of males.…”

Section: Compilation Of Toxicity Data For Fadrozole and Prochlorazmentioning

confidence: 99%

Species extrapolation for the 21st century

Celander

Goldstone

Denslow

et al. 2011

Environmental Toxicology and Chemistry

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Safety factors are used in ecological risk assessments to extrapolate from the toxic responses of laboratory test species to all species representing that group in the environment. More accurate extrapolation of species responses is important. Advances in understanding the mechanistic basis for toxicological responses and identifying molecular response pathways can provide a basis for extrapolation across species and, in part, an explanation for the variability in whole organism responses to toxicants. We highlight potential short- and medium-term development goals to meet our long-term aspiration of truly predictive in silico extrapolation across wildlife species' response to toxicants. A conceptual approach for considering cross-species extrapolation is presented. Critical information is required to establish evidence-based species extrapolation, including identification of critical molecular pathways and regulatory networks that are linked to the biological mode of action and species' homologies. A case study is presented that examines steroidogenesis inhibition in fish after exposure to fadrozole or prochloraz. Similar effects for each compound among fathead minnow, medaka, and zebrafish were attributed to similar inhibitor pharmacokinetic/pharmacodynamic distributions and sequences of cytochrome P45019A1/2 (CYP19A1/2). Rapid advances in homology modeling allow the prediction of interactions of chemicals with enzymes, for example, CYP19 aromatase, which would eventually allow a prediction of potential aromatase toxicity of new compounds across a range of species. Eventually, predictive models will be developed to extrapolate across species, although substantial research is still required. Knowledge gaps requiring research include defining differences in life histories (e.g., reproductive strategies), understanding tissue-specific gene expression, and defining the role of metabolism on toxic responses and how these collectively affect the power of interspecies extrapolation methods.

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Effects of the fungicide prochloraz on the sexual development of zebrafish (Danio rerio)

Cited by 66 publications

References 33 publications

The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations

The progestin levonorgestrel affects sex differentiation in zebrafish at environmentally relevant concentrations

Influence of Propylparaben on Vitellogenesis and Sex Ratio in Juvenile Zebrafish (Danio rerio)

Species extrapolation for the 21st century

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