Effects of the Geometrical Structure of a Honeycomb TCP on Relationship between Bone / Cartilage Formation and Angiogenesis

Matsuda, Hiroyuki; Takabatake, Kiyofumi; Tsujigiwa, Hidetsugu; Watanabe, Satoko; Ito, Satoshi; Kawai, Hiroki; Hamada, Mei; Yoshida, Saori; Nakano, Keisuke; Nagatsuka, Hitoshi

doi:10.7150/ijms.28452

Cited by 9 publications

(9 citation statements)

References 26 publications

(24 reference statements)

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“…We have confirmed that honeycomb β-TCP, in which linear through-holes with pore diameters of 300 µm are arranged in a honeycomb shape, has very high osteoinductive ability [18]. We also reported that this honeycomb β-TCP, which can reproduce the microenvironment, can control cell differentiation by changing the geometrical structure and enable differentiation of chondrocytes and osteoblasts [19]. Moreover, when we used a rat zygomatic defect model and implanted honeycomb β-TCP into the cheekbone defect, we obtained good bone fusion with the zygomatic stump bone.…”

Section: Introductionsupporting

confidence: 65%

Effect of Honeycomb β-TCP Geometrical Structure on Bone Tissue Regeneration in Skull Defect

et al. 2020

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The effect of the geometric structure of artificial biomaterials on skull regeneration remains unclear. In a previous study, we succeeded in developing honeycomb β-tricalcium phosphate (β-TCP), which has through-and-through holes and is able to provide the optimum bone microenvironment for bone tissue regeneration. We demonstrated that β-TCP with 300-μm hole diameters induced vigorous bone formation. In the present study, we investigated how differences in hole directions of honeycomb β-TCP (horizontal or vertical holes) influence bone tissue regeneration in skull defects. Honeycomb β-TCP with vertical and horizontal holes was loaded with BMP-2 using Matrigel and Collagen gel as carriers, and transplanted into skull bone defect model rats. The results showed that in each four groups (Collagen alone group, Matrigel alone group, Collagen + BMP group and Matrigel + BMP-2), vigorous bone formation was observed on the vertical β-TCP compared with horizontal β-TCP. The osteogenic area was larger in the Matrigel groups (with and without BMP-2) than in the Collagen group (with and without BMP-2) in both vertical β-TCP and horizontal β-TCP. However, when BMP-2 was added, the bone formation area was not significantly different between the Collagen group and the Matrigel group in the vertical β-TCP. Histological finding showed that, in vertical honeycomb β-TCP, new bone formation extended to the upper part of the holes and was observed from the dura side to the periosteum side as added to the inner walls of the holes. Therefore, we can control efficient bone formation by creating a bone microenvironment provided by vertical honeycomb β-TCP. Vertical honeycomb β-TCP has the potential to be an excellent biomaterial for bone tissue regeneration in skull defects and is expected to have clinical applications.

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Section: Introductionsupporting

confidence: 65%

Effect of Honeycomb β-TCP Geometrical Structure on Bone Tissue Regeneration in Skull Defect

et al. 2020

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“…In addition, by changing the pore diameter of honeycomb TCP, we have reproduced the hard tissue formation microenvironment and succeeded in specifically inducing and forming cartilage. Specifically, cartilage formation was observed in honeycomb TCP with a 75-μm pore size in rat femoral muscle [ 24 ]. Therefore, this honeycomb β-TCP, which can reproduce the microenvironment, can control cell differentiation by changing the geometrical structure and enabling differentiation of chondrocytes and osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

A Pilot Study of Seamless Regeneration of Bone and Cartilage in Knee Joint Regeneration Using Honeycomb TCP

et al. 2021

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The knee joint is a continuous structure of bone and cartilage tissue, making it difficult to regenerate using artificial biomaterials. In a previous study, we succeeded in developing honeycomb tricalcium phosphate (TCP), which has through-and-through holes and is able to provide the optimum microenvironment for hard tissue regeneration. We demonstrated that TCP with 300 μm pore diameters (300TCP) induced vigorous bone formation, and that TCP with 75 μm pore diameters (75TCP) induced cartilage formation. In the present study, we regenerated a knee joint defect using honeycomb TCP. 75TCP and 300TCP were loaded with transforming growth factor (TGF)-β alone or bone morphogenic protein (BMP)-2+TGF-β with or without Matrigel and transplanted into knee joint defect model rabbits. 75TCP showed no bone or cartilage tissue formation in any of the groups with TGF-β alone and BMP-2+TGF-β with/without Matrigel. However, for 300TCP and BMP-2+TGF-β with or without Matrigel, vigorous bone tissue formation was observed in the TCP holes, and cartilage tissue formation in the TCP surface layer was continuous with the existing cartilage. The cartilage area in the TCP surface was larger in the group without Matrigel (with BMP-2+TGF-β) than in the group with Matrigel (with BMP-2+TGF-β). Therefore, honeycomb TCP can induce the seamless regeneration of bone and cartilage in a knee joint.

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“…Furthermore, they had a polar arrangement and exhibited odontoblast-like structures as if they were found in the pulp cavity. In our previous study, the angiogenesis regulated by the pore size of honeycomb TCP influenced on hard tissue formation [ 14 ]. The previous study indicated that the invasive blood vessel formation pattern in 300TCP which showed capillary pattern was different from that in 500TCP, which showed the same linear angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, we reported that the surface properties resulting from the different sintering temperature affect the osteoinductivity and biocompatibility of TCP [ 13 ]. Furthermore, changing the through-holes diameters of honeycomb TCP holes allows for successful control of cartilage and bone formation [ 14 ]. In particular, in the skull defect model rat, vigorous bone tissue formation was observed in honeycomb TCP containing through-and-through holes with diameters of 300 μm, suggesting its clinical applicability [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Geometrical Structure of Honeycomb TCP to Control Dental Pulp-Derived Cell Differentiation

et al. 2020

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Recently, dental pulp has been attracting attention as a promising source of multipotent mesenchymal stem cells (MSCs) for various clinical applications of regeneration fields. To date, we have succeeded in establishing rat dental pulp-derived cells showing the characteristics of odontoblasts under in vitro conditions. We named them Tooth matrix-forming, GFP rat-derived Cells (TGC). However, though TGC form massive dentin-like hard tissues under in vivo conditions, this does not lead to the induction of polar odontoblasts. Focusing on the importance of the geometrical structure of an artificial biomaterial to induce cell differentiation and hard tissue formation, we previously have succeeded in developing a new biomaterial, honeycomb tricalcium phosphate (TCP) scaffold with through-holes of various diameters. In this study, to induce polar odontoblasts, TGC were induced to form odontoblasts using honeycomb TCP that had various hole diameters (75, 300, and 500 μm) as a scaffold. The results showed that honeycomb TCP with 300-μm hole diameters (300TCP) differentiated TGC into polar odontoblasts that were DSP positive. Therefore, our study indicates that 300TCP is an appropriate artificial biomaterial for dentin regeneration.

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Effects of the Geometrical Structure of a Honeycomb TCP on Relationship between Bone / Cartilage Formation and Angiogenesis

Cited by 9 publications

References 26 publications

Effect of Honeycomb β-TCP Geometrical Structure on Bone Tissue Regeneration in Skull Defect

Effect of Honeycomb β-TCP Geometrical Structure on Bone Tissue Regeneration in Skull Defect

A Pilot Study of Seamless Regeneration of Bone and Cartilage in Knee Joint Regeneration Using Honeycomb TCP

Geometrical Structure of Honeycomb TCP to Control Dental Pulp-Derived Cell Differentiation

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