Effects of the herbicide atrazine on the activated sludge process: microbiology and functional views

Nsabimana, E; Bohatier, Jacques; Belan, André; Pepirr, D.; Charles, Laurence

doi:10.1016/0045-6535(96)00182-8

Cited by 12 publications

(6 citation statements)

References 28 publications

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“…Since these three compounds are structurally similar, the e$ciency of degradation may be related to the size of the alkyl chain. Desethylatrazine and hydroxyatrazine appear as metabolites of atrazine in water (Mills and Thurmann, 1994;Nsabimana et al, 1996;Papilloud et al, 1996;Yanze-Kontchou and Gschwind, 1994). Although desethylatrazine was present in the culture supernatant, its degradation rate must be higher than that estimated, because it can be an intermediate of atrazine, simazine, and terbutylazine degradation.…”

Section: Discussionmentioning

confidence: 99%

“…A similar process was also proposed by Somich et al (1990) to remediate waste and rinsate contaminated with atrazine. For wastewater, Nsabimana et al (1996) showed that 20 mg/liter of atrazine had an immediate e!ect on chemical oxygen demand (COD), and after 4 days the bacterial concentration of the sludge was reduced. These authors also found that among the six bacterial strains isolated from sludges, none degraded atrazine.…”

Section: Discussionmentioning

confidence: 99%

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Biodegradation ofs-Triazine Compounds by a Stable Mixed Bacterial Community

Cy¹,

Gschwind²

1999

Ecotoxicology and Environmental Safety

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biodegradation ofs-Triazine Compounds by a Stable Mixed Bacterial Community

Cy¹,

Gschwind²

1999

Ecotoxicology and Environmental Safety

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“…Under the same conditions, the same CAS could run stably over 4 weeks without sludge bulking without atrazine exposure. On the other hand, atrazine had shown some toxicity on the bacteria in the activated sludge [36]. The process of sludge bulking could be described as follows: sludge flocs size became smaller and sludge sedimentation became worse, resulting in more and more sludge flocs escaping from CAS with effluent.…”

Section: Discussionmentioning

confidence: 98%

Enhanced atrazine removal using membrane bioreactor bioaugmented with genetically engineered microorganism

Huang

2008

Front. Environ. Sci. Eng. China

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Bioaugmentation with genetically engineered microorganisms (GEMs) in a membrane bioreactor (MBR) for enhanced removal of recalcitrant pollutants was explored. An atrazine-degrading genetically engineered microorganism (GEM) with green fluorescent protein was inoculated into an MBR and the effects of such a bioaugmentation strategy on atrazine removal were investigated. The results show that atrazine removal was improved greatly in the bioaugmented MBR compared with a control system. After a start-up period of 6 days, average 94.7% of atrazine was removed in bioaugmented MBR when atrazine concentration of influent was 14.5 mg/L. The volumetric removal rates increased linearly followed by atrazine loading increase and the maximum was 65.5 mg/(L?d). No negative effects were found on COD removal although carbon oxidation activity of bioaugmented sludge was lower than that of common sludge. After inoculation, adsorption to sludge flocs was favorable for GEM survival. The GEM population size initially decreased shortly and then was kept constant at about 10 4 -10 5 CFU/mL. Predation of micro-organisms played an important role in the decay of the GEM population. GEM leakage from MBR was less than 10 2 CFU/mL initially and was then undetectable. In contrast, in a conventionally activated sludge bioreactor (CAS), sludge bulking occurred possibly due to atrazine exposure, resulting in bioaugmentation failure and serious GEM leakage. So MBR was superior to CAS in atrazine bioaugmentation treatment using GEM.

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“…After in silico specificity analysis, eight non-nitrifying bacteria including ones frequently found in wastewater treatment plants Manefield et al, 2005;Nsabimana et al, 1996;Tchobanoglous and Burton, 1991) were also purchased to test the potential false positive detection. The bacteria were Acinetobacter calcoaceticus KCTC 2357 T (A. cal), Aeromonas hydrophila subsp.…”

Section: Sources and Extraction Of Dnamentioning

confidence: 99%

Primer and probe sets for group‐specific quantification of the genera Nitrosomonas and Nitrosospira using real‐time PCR

Lim

Shin

et al. 2007

Biotech & Bioengineering

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Use of quantitative real-time PCR (QPCR) with TaqMan probes is increasingly popular in various environmental works to detect and quantify a specific microorganism or a group of target microorganism. Although many aspects of conducting a QPCR assay have become very easy to perform, a proper design of oligonucleotide sequences comprising primers and a probe is still considered as one of the most important aspects of a QPCR application. This work was conducted to design group specific primer and probe sets for the detection of ammonia oxidizing bacteria (AOB) using a real-time PCR with a TaqMan system. The genera Nitrosomonas and Nitrosospira were grouped into five clusters based on similarity of their 16S rRNA gene sequences. Five group-specific AOB primer and probe sets were designed. These sets separately detect four subgroups of Nitrosomonas (Nitrosomonas europaea-, Nitrosococcus mobilis-, Nitrosomonas nitrosa-, and Nitrosomonas cryotolerans-clusters) along with the genus Nitrosospira. Target-group specificity of each primer and probe set was initially investigated by analyzing potential false results in silico, followed by a series of experimental tests for QPCR efficiency and detection limit. In general, each primer and probe set was very specific to the target group and sensitive to detect target DNA as low as two 16S rRNA gene copies per reaction mixture. QPCR efficiency, higher than 93.5%, could be achieved for all primer and probe sets. The primer and probe sets designed in this study can be used to detect and quantify the beta-proteobacterial AOB in biological nitrification processes and various environments.

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Effects of the herbicide atrazine on the activated sludge process: microbiology and functional views

Cited by 12 publications

References 28 publications

Biodegradation ofs-Triazine Compounds by a Stable Mixed Bacterial Community

Biodegradation ofs-Triazine Compounds by a Stable Mixed Bacterial Community

Enhanced atrazine removal using membrane bioreactor bioaugmented with genetically engineered microorganism

Primer and probe sets for group‐specific quantification of the genera Nitrosomonas and Nitrosospira using real‐time PCR

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