Effects of the insecticide amitraz, an α2-adrenergic receptor agonist, on human luteinized granulosa cells

Young, Fiona; Menadue, Margaret; Lavranos, Tina C.

doi:10.1093/humrep/dei194

Cited by 25 publications

(20 citation statements)

References 25 publications

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“…42 Norepinephrine may then be able to interact with ovarian a-and b-receptors present on granulose cells. 43,44 Bovine luteal cells are also reported to synthesize NE in a similar way 45 and presumably this also occurs in intraovarian nerve fibres. Recent study revealed dopamine receptors expressed by endocrine cells of the follicle and the corpus luteum, which suggests a complex role of dopamine in the regulation of ovarian processes.…”

Section: Discussionmentioning

confidence: 94%

Impact of 2 Doses of Clorgyline on the Rat Preimplantation Embryo Development and the Monoamine Levels in Urine

et al. 2010

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We have evaluated the impact of chronic administration of clorgyline, a potent monoamine oxidase A inhibitor and a former antidepressant, on the preimplantation embryo development in Wistar rats. Females were injected intraperitoneally daily for 30 days with saline (control animals), or with a low-dose clorgyline (LDC, 0.1 mg/kg per d) or with a high-dose clorgyline (HDC, 1 mg/kg per d). Embryos were isolated on day 5 of pregnancy and urine was collected by puncture of the urinary bladder. The number of embryos per female did not differ between experimental groups and control, but we have recorded a decreased number of embryos in HDC group compared to LDC (P < .05). We have found that LDC significantly reduced the presence of healthy embryos and increased the presence of the degenerated embryos (P < .001). The administration of the LDC resulted in the lowest cell number in blastocysts. We have observed significantly increased serotonin levels in HDC group compared to both control (P < .05) and LDC animals (P < .01). Norepinephrine (NE) levels in both experimental groups were significantly elevated compared to controls. Dopamine levels did not differ between groups (P > .05). We speculate that lesser negative effect of HDC compared to LDC on the preimplantation embryo development could be the consequence of the lower NE levels and/or elevated serotonin levels. Potential mechanisms mediating clorgyline-induced impaired preimplantation embryo development are proposed.

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Section: Discussionmentioning

confidence: 94%

Impact of 2 Doses of Clorgyline on the Rat Preimplantation Embryo Development and the Monoamine Levels in Urine

et al. 2010

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“…The concentration of P 4 in the cell culture medium after treatments was measured by radioimmunoassay (RIA) as previously described . Briefly, an antibody against P 4 raised in rabbit and 125 I‐radiolabeled P 4 (MP Biomedical; Solon, OH, USA), along with a sheep antirabbit secondary antibody (Chemicon; Boronia, Victoria, Australia), were used for the measurement of P 4 .…”

Section: Methodsmentioning

confidence: 99%

An in vitro high‐throughput assay for screening reproductive and toxic effects of anticancer compounds

Edwards

Benkendorff

Young

2014

Biotech and App Biochem

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An in vitro assay was developed that simultaneously tested the effects of anticancer drug candidates on cytotoxicity, hormone synthesis, and gonadotrophin responsiveness using the choriocarcinoma JAr cell line. JAr culture conditions were optimized and then cells were exposed to a marine mollusc extract in the presence and absence of hCG. The intra- and interassay coefficients of variation of the optimized 1 H thiazolyl blue tetrazolium bromide assay were 11.3% and 10.9%, respectively. hCG (1,000 mIU/mL) increased progesterone (P4) synthesis after 24 H (P<0.05). The mollusc extract significantly decreased cell viability, with the IC50 affected by incubation time, but not hCG. P4 synthesis was inhibited at low concentrations of the anticancer extract, but stimulated at the highest concentration, and complex interactions of P4 were also found with hCG. In conclusion, the optimized assay is useful to characterize the effects of novel drugs on cytotoxicity, basal, and gonadotrophin-stimulated P4 synthesis in vitro, and can be used to inform subsequent in vivo studies.

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“…Granulosa cells were donated by women with normal reproductive physiology who were undergoing ART to treat male infertility. Granulosa cell isolation has been described previously [53]. Briefly, pooled aspirates for each woman were isolated by centrifugation at 107× g for 10 min, followed by two cycles of rinsing and then the cell suspension was re-suspended in Dulbecco’s modified Eagle’s HAMS F12 (DMEM-F12; GIBCO, Invitrogen Corporation) mediumsupplemented with 10% FBS (GIBCO Invitrogen Corporation), penicillin/streptomycin 5000 IU/mL and 5000 μg/mL respectively (Thermo Scientific), insulin (5 μg/mL), transferin apohuman (5 μg/mL), selenium sodium selenite (5 ng/mL) buffered with 1.2 g/L NaHCO 3 (Pfizer).…”

Section: Methodsmentioning

confidence: 99%

Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells

2012

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Anticancer properties of tyrindoleninone and 6-bromoisatin from Dicathais orbita were tested against physiologically normal primary human granulosa cells (HGC) and reproductive cancer cell lines. Tyrindoleninone reduced cancer cell viability with IC50 values of 39 µM (KGN; a tumour-derived granulosa cell line), 39 μM (JAr), and 156 μM (OVCAR-3), compared to 3516 μM in HGC. Apoptosis in HGC’s occurred after 4 h at 391 µM tyrindoleninone compared to 20 µM in KGN cells. Differences in apoptosis between HGC and KGN cells were confirmed by TUNEL, with 66 and 31% apoptotic nuclei at 4 h in KGN and HGC, respectively. These marine compounds therefore have potential for development as treatments for female reproductive cancers.

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Effects of the insecticide amitraz, an α2-adrenergic receptor agonist, on human luteinized granulosa cells

Abstract: Amitraz inhibited basal and hCG-stimulated progesterone but not estrogen production. The inhibitory action of amitraz and its antagonism by yohimbine suggest that alpha2-adrenergic receptors are expressed by luteinized human granulosa cells.

Cited by 25 publications

References 25 publications

Impact of 2 Doses of Clorgyline on the Rat Preimplantation Embryo Development and the Monoamine Levels in Urine

Impact of 2 Doses of Clorgyline on the Rat Preimplantation Embryo Development and the Monoamine Levels in Urine

An in vitro high‐throughput assay for screening reproductive and toxic effects of anticancer compounds

Marine Compounds Selectively Induce Apoptosis in Female Reproductive Cancer Cells but Not in Primary-Derived Human Reproductive Granulosa Cells

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