Effects of the maize C4 phosphoenolpyruvate carboxylase (ZmPEPC) gene on nitrogen assimilation in transgenic wheat

Peng, Chaojun; Xu, Weina; Hu, Lin; Li, Yan; Qi, Xueli; Wang, Huiwei; Hua, Xia; Zhao, Ming

doi:10.1007/s10725-017-0332-x

Cited by 11 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were observed with transgenic wheat and rice overexpressing maize and sugarcane C 4 -specific PEPC gene, respectively (Lian et al 2014;Shen et al 2015). The overexpression of Zea mays PEPC gene in Arabidopsis thaliana, and wheat plants showed high PEPC activity and improved photosynthesis, growth, and yield under salinity and low nitrogen conditions by modulating carbon metabolism and nitrogen assimilation (Kandoi et al 2016;Peng et al 2018). Earlier, it was noticed that overexpression of maize PEPC gene in rice reduces the carbon assimilation rate (7.3%) but improves photorespiration in transgenic plants (Fukayama et al 2003).…”

Section: Overexpression Of C 4 Pepc Enzyme-encoding Gene In C 3 Plantssupporting

confidence: 65%

“…Based on the advantages of C 4 carbon assimilation over the limited factors of C 3 plants, introgression of C 4 photosynthetic enzymes in C 3 plants are a reliable, cost-effective and prospective approach to enhance the productivity and biomass to improve the photosynthesis of C 3 plants and also biological CO 2 sequestration (Edwards et al 2001;Leegood 2002). The C 4 photosynthetic genes encoding for enzymes PEPC, PPDK, or NADP-ME were overexpressed in tobacco (Laporte et al 2002;Mu ¨ller et al 2018), potato (Ishimaru et al 1998;Ha ¨usler et al 2001), rice (Taniguchi et al 2008;Gu et al 2013;Shen et al 2015), wheat (Kershanskaya and Teixeira da Silva 2010;Peng et al 2018;) and Arabidopsis (Fahnenstich et al 2007;Kandoi et al 2016).…”

Section: Overexpression Of C 4 Photosynthetic Genesmentioning

confidence: 99%

See 1 more Smart Citation

Ectopic expression of C4 photosynthetic pathway genes improves carbon assimilation and alleviate stress tolerance for future climate change

Yadav

Mishra

2020

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Alteration in atmospheric carbon dioxide concentration and other environmental factors are the significant cues of global climate change. Environmental factors affect the most fundamental biological process including photosynthesis and different metabolic pathways. The feeding of the rapidly growing world population is another challenge which imposes pressure to improve productivity and quality of the existing crops. C 4 plants are considered the most productive, containing lower photorespiration, and higher water-use & N-assimilation efficiencies, compared to C 3 plants. Besides, the C 4 -photosynthetic genes not only play an important role in carbon assimilation but also modulate abiotic stresses. In this review, fundamental three metabolic processes (C 4 , C 3 , and CAM) of carbon dioxide assimilation, the evolution of C 4 -photosynthetic genes, effect of elevated CO 2 on photosynthesis, and overexpression of C 4 -photosynthetic genes for higher photosynthesis were discussed. Kranz-anatomy is considered an essential prerequisite for the terrestrial C 4 carbon assimilation, but single-celled C 4 plant species changed this well-established paradigm. C 4 plants are insensitive to an elevated CO 2 stress condition but performed better under stress conditions. Overexpression of essential C 4photosynthetic genes such as PEPC, PPDK, and NADP-ME in C 3 plants like Arabidopsis, tobacco, rice, wheat, and potato not only improved photosynthesis but also provided tolerance to various environmental stresses, especially drought. The review provides useful information for sustainable productivity and yield under elevated CO 2 environment, which to be explored further for CO 2 assimilation and also abiotic stress tolerance. Additionally, it provides a better understanding to explore C 4 -photosynthetic gene(s) to cope with global warming and prospective adverse climatic changes.

show abstract

Section: Overexpression Of C 4 Pepc Enzyme-encoding Gene In C 3 Plantssupporting

confidence: 65%

Section: Overexpression Of C 4 Photosynthetic Genesmentioning

confidence: 99%

Ectopic expression of C4 photosynthetic pathway genes improves carbon assimilation and alleviate stress tolerance for future climate change

Yadav

Mishra

2020

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…At the same time, Pantoea can also enhance plant drought resistance [106,107]. Corynebacterium has also shown its ability to improve the photosynthetic capacity of crops [108,109]. Chryseobacterium can solubilize phosphates, and its combined use with nitrogen and phosphorus fertilizers can promote plant growth and increase crop yield [110]; studies have also shown that Chryseobacterium can increase the content of photosynthetic pigments in plant leaves and produce indole-3-acetic acid [111,112].…”

Section: Discussionmentioning

confidence: 99%

ZnO NPs Enhanced Photosynthetic Capacity, Promoted New Shoot Development, and Improved the Community Composition of Phyllosphere Epiphytic and Endophytic Microorganisms in Tea Plants

Chen,

Song,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Nanotechnology holds revolutionary potential in the field of agriculture, with zinc oxide nanoparticles (ZnO NPs) demonstrating advantages in promoting crop growth. Photosynthesis is a key process in the growth and quality formation of tea plants, and phyllosphere microorganisms also have a significant impact on plant growth and health. However, the effects of ZnO NPs on the photosynthesis of tea plants, the sprouting of new shoots, and the community of phyllosphere microorganisms are not yet clear. Results This study investigated the photosynthetic physiological parameters of tea plants under the influence of ZnO NPs, the content of key photosynthetic enzymes such as RubisCO, chlorophyll content, chlorophyll fluorescence parameters, transcriptomes (leaves and new shoots), extensively targeted metabolomes (leaves and new shoots), mineral element content (leaves and new shoots), and the communities of epiphytic and endophytic microorganisms in the phyllosphere. The results indicated that ZnO NPs could enhance the photosynthesis of tea plants, upregulate the expression of some genes related to photosynthesis, increase the accumulation of photosynthetic products, promote the development of new shoots, and alter the content of various mineral elements in the leaves and new shoots of tea plants. Additionally, ZnO NPs improved the community composition of epiphytic and endophytic microorganisms in the phyllosphere of tea plants, inhibited potential pathogenic microorganisms, and allowed various beneficial microorganisms with potential growth-promoting properties to become dominant species. Conclusion This study demonstrates that ZnO NPs have a positive impact on the photosynthesis of tea plants, the sprouting of new shoots, and the community of phyllosphere microorganisms, which can improve the growth condition of tea plants. These findings provide new scientific evidence for the application of ZnO NPs in sustainable agricultural development and contribute to advancing research in nanobiotechnology aimed at enhancing crop yield and quality.

show abstract

“…In the C4 pathway, the receptor for CO 2 is phosphoenolpyruvate (PEP), and the fixation of CO 2 occurs in the cytoplasm of mesophyll cells. PEPC catalyzes the reaction of PEP with CO 2 to form some C4 acids, such as oxaloacetate and malic acid; these are decarboxylated after they are transferred to bundle sheath cells, and the Rubisco fixes the released carbon dioxide again [ 40 , 41 ]. The mesophyll cells of the maize parenchyma contain sheath cells, and they can form a Kranz structure that acts as a CO 2 pump.…”

Section: Discussionmentioning

confidence: 99%

Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize–Peanut Intercropping System

Ma,

Feng,

Wang

et al. 2023

Plants

View full text Add to dashboard Cite

Intercropping is a traditional and sustainable planting method that can make rational use of natural resources such as light, temperature, fertilizer, water, and CO2. Due to its efficient resource utilization, intercropping, in particular, maize and legume intercropping, is widespread around the world. However, the molecular details of these pathways remain largely unknown. In this study, physiological, transcriptome, and proteome analyses were compared between maize monocropping and maize–peanut intercropping. The results show that an intercropping system enhanced the ability of carbon fixation and carboxylation of maize leaves. Apparent quantum yield (AQY), the light-saturated net photosynthetic rate (LSPn), the light saturation point (LSP), and the light compensation point (LCP) were increased by 11.6%, 9.4%, 8.9%, and 32.1% in the intercropping system, respectively; carboxylation efficiency (CE), the CO2 saturation point (Cisat), the Rubisco maximum carboxylation rate (Vcmax), the maximum electron transfer rate (Jmax), and the triose phosphate utilization rate (TPU) were increased by 28.5%, 7.3%, 18.7%, 29.2%, and 17.0%, respectively; meanwhile, the CO2 compensation point (Γ) decreased by 22.6%. Moreover, the transcriptome analysis confirmed the presence of 588 differentially expressed genes (DEGs), and the numbers of up-regulated and down-regulated genes were 383 and 205, respectively. The DEGs were primarily concerned with ribosomes, plant hormone signal transduction, and photosynthesis. Furthermore, 549 differentially expressed proteins (DEPs) were identified in the maize leaves in both the maize monocropping and maize–peanut intercropping systems. Bioinformatics analysis revealed that 186 DEPs were related to 37 specific KEGG pathways in each of the two treatment groups. Based on the physiological, transcriptome, and proteome analyses, it was demonstrated that the photosynthetic characteristics in maize leaves can be improved by maize–peanut intercropping. This may be related to PS I, PS II, cytochrome b6f complex, ATP synthase, and photosynthetic CO2 fixation, which is caused by the improved CO2 carboxylation efficiency. Our results provide a more in-depth understanding of the high yield and high-efficiency mechanism in maize and peanut intercropping.

show abstract

Effects of the maize C4 phosphoenolpyruvate carboxylase (ZmPEPC) gene on nitrogen assimilation in transgenic wheat

Cited by 11 publications

References 49 publications

Ectopic expression of C4 photosynthetic pathway genes improves carbon assimilation and alleviate stress tolerance for future climate change

Ectopic expression of C4 photosynthetic pathway genes improves carbon assimilation and alleviate stress tolerance for future climate change

ZnO NPs Enhanced Photosynthetic Capacity, Promoted New Shoot Development, and Improved the Community Composition of Phyllosphere Epiphytic and Endophytic Microorganisms in Tea Plants

Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize–Peanut Intercropping System

Contact Info

Product

Resources

About