Effects of the MC4R, CAPN1, and ADSL genes on body weight and purine content in slow-growing chickens

Kubota, Shin; Vandee, Achiraya; Keawnakient, Porntiwa; Molee, Wittawat; Yongsawatdikul, Jirawat; Molee, Amonrat

doi:10.3382/ps/pez262

Cited by 27 publications

(37 citation statements)

References 51 publications

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“…In broiler, increased skeletal muscle fiber diameter and area could increase shear force and reduce meat tenderness [ 58 ]. A significant association has been reported between the genotype of the CAPN1 gene and breast muscle fiber diameter in KRC, and KRCs carrying the BB genotype showed a significantly smaller muscle fiber diameter at 10 weeks of age [ 59 ]. Genetic selection of female KRCs for large muscle fiber size combined with feeding them diets supplemented with β-alanine or L-histidine may produce meat containing high carnosine concentrations without losing the toughness unique to this meat.…”

Section: Discussionmentioning

confidence: 99%

RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine

Kubota

Promkhun

Sinpru

et al. 2021

Animals

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Korat chicken (KRC) is a slow-growing chicken bred in Thailand, whose meat exhibits a unique toughness. A previous study produced KRC breast meat containing high carnosine content through dietary supplementation with β-alanine or L-histidine; however, the KRC that were fed an L-histidine-supplemented diet produced meat that was significantly more tender. Herein, we performed RNA-Seq to identify candidate genes involved in the regulation of carnosine content and meat toughness. Total RNA was isolated from five female KRC breast muscles in each treatment group that KRC fed diets without supplementation, supplemented with β-alanine or L-histidine. Compared to the non-supplemented group, we identified 118 and 198 differentially expressed genes (DEGs) in the β-alanine or L-histidine supplementation groups, respectively. Genes potentially related to meat tenderness—i.e., those regulating myosin, collagen, intramuscular fat, and calpain—were upregulated (LOC107051274, ACSBG1, and CAPNS2) and downregulated (MYO7B, MYBPH, SERPINH1, and PGAM1). However, carnosine synthase gene was not identified. Functional enrichment analysis identified pathways affected by dietary supplementation, including the insulin signaling pathway (β-alanine supplementation) and the insulin resistance and adipocytokine signaling pathways (L-histidine supplementation). The FoxO signaling pathway was identified as a regulatory network for both supplementation groups. The identified genes can be used as molecular markers of meat tenderness in slow-growing chickens.

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Section: Discussionmentioning

confidence: 99%

RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine

Kubota

Promkhun

Sinpru

et al. 2021

Animals

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“…For the KR chickens that we studied, FCR was 1.93 in the low-FCR group and 3.29 in the high-FCR group. In a KR population, the market-age FCR averages 2.2 [ 12 ], which is below that of commercial slow-growing meat-type chickens, at 3.22 [ 40 ], and Chinese yellow slow-growing chickens, at 3.15 [ 7 , 40 ]. We observed that, for KR chickens, higher body weight can be achieved with reduced feed intake, even at FCR values as low as 1.93.…”

Section: Discussionmentioning

confidence: 99%

“…KR meat has a unique taste—compared to broiler meat it is firmer and chewier, with lower fat and higher collagen content, giving it a higher selling price [ 10 , 11 ]. KR chickens have an average daily weight gain of 19.8–21.0 g/d, and FCR of 2.2–2.3 [ 12 ]; they are sent to the market at ca. 1.2–1.7 kg bodyweight, at 10 weeks of age.…”

Section: Introductionmentioning

confidence: 99%

Jejunal Transcriptomic Profiling for Differences in Feed Conversion Ratio in Slow-Growing Chickens

Sinpru

Riou

Kubota

et al. 2021

Animals

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Improving feed efficiency is an important breeding target for the poultry industry; to achieve this, it is necessary to understand the molecular basis of feed efficiency. We compared the jejunal transcriptomes of low- and high-feed conversion ratio (FCR) slow-growing Korat chickens (KRs). Using an original sample of 75 isolated 10-week-old KR males, we took jejunal samples from six individuals in two groups: those with extremely low FCR (n = 3; FCR = 1.93 ± 0.05) and those with extremely high FCR (n = 3; FCR = 3.29 ± 0.06). Jejunal transcriptome profiling via RNA sequencing revealed 56 genes that were differentially expressed (p < 0.01, FC > 2): 31 were upregulated, and 25 were downregulated, in the low-FCR group relative to the high-FCR group. Functional annotation revealed that these differentially expressed genes were enriched in biological processes related to immune response, glutathione metabolism, vitamin transport and metabolism, lipid metabolism, and neuronal and cardiac maturation, development, and growth, suggesting that these are important mechanisms governing jejunal feed conversion. These findings provide an important molecular basis for future breeding strategies to improve slow-growing chicken feed efficiency.

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“…As a result, both genes were not affected by selection and were in HWE. From Kubota et al (2019) explain that HWE was influenced by many factors, including selection, the rate of recombination and mutation, genetic drift, the mating system, population structure, and genetic linkage.…”

Section: Egg Production In High Egg Strain Of Pradu Hangdum Chiangmai Chickens (Hphc)mentioning

confidence: 99%

The Association of Dopamine Receptor D2 (DRD2) and Vasoactive Intestinal Peptide (VIP) Polymorphisms on Egg Production in High Egg Strain of Pradu Hangdum Chiangmai Chickens

Ruangwittayanusorn¹,

Promket²,

Pimrueng³

et al. 2022

Adv. Anim. Vet. Sci.

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The objectives of the research were to determine the association of Dopamine Receptor D2 type (DRD2) and Vasoactive Intestinal Peptide (VIP) on egg production in 281 high egg strain of Pradu Hangdum Chiangmai chickens (HPHC). PCR-RFLP protocols were used to identify polymorphisms of both genes. The frequencies of TT, TC and CC genotypes of DRD2 were 0.05, 0.40, and 0.55, respectively. The frequencies of T and C alleles of DRD2 were 0.25 and 0.75, respectively. Regarding VIP, the frequencies of II, ID and DD genotypes were 0.59, 0.28, and 0.13, respectively. The frequencies of I and D alleles of VIP were 0.73 and 0.27, respectively. The association of DRD2 polymorphism were detected on egg number at 360 days (EN360) and egg per month (EM) (P<0.05). The chickens carrying the CC genotype had higher EN360 and EM (189.82 eggs and 15.81 eggs) and the ones carrying the TC (180.98 eggs and 15.08 eggs) and the TT genotype (176.38 eggs and 14.69 eggs) was non-significant. The VIP polymorphism were associated on egg number at 270 days (EN270), EN360 and EM (P<0.01). The DD genotype had higher EN270, EN360 and EM when compared to the ID and II genotypes. The DRD2 and VIP genes were moderate polymorphism and their alleles could be the potential genetic markers for chickens' selection to improve egg number.

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Effects of the MC4R, CAPN1, and ADSL genes on body weight and purine content in slow-growing chickens

Cited by 27 publications

References 51 publications

RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine

RNA Profiles of the Korat Chicken Breast Muscle with Increased Carnosine Content Produced through Dietary Supplementation with β-Alanine or L-Histidine

Jejunal Transcriptomic Profiling for Differences in Feed Conversion Ratio in Slow-Growing Chickens

The Association of Dopamine Receptor D2 (DRD2) and Vasoactive Intestinal Peptide (VIP) Polymorphisms on Egg Production in High Egg Strain of Pradu Hangdum Chiangmai Chickens

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