Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples

Gino, Sarah; Omedei, M.

doi:10.1016/j.fsigss.2011.08.133

Cited by 12 publications

(9 citation statements)

References 7 publications

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“…With fingermarks enhancement, several studies [49][50][51][52][53][54] have shown that most of the reagents commonly used to develop latent marks, such as aluminium powder, black magnetic powder, fluorescent powders, wet powder suspension and 1,2-indanedione do not affect subsequent recovery of DNA. Other specific chemicals such as cyanoacrylate and ninhydrin may have a negative impact on the process as they can either degrade or sequester the DNA or inhibit the PCR reaction [55,56].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The effect of mark enhancement techniques on the presumptive and confirmatory tests for blood

et al. 2018

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An investigation into the effects of physical and chemical enhancement on subsequent presumptive and confirmatory tests for human blood is presented. Human blood was deposited onto porous (white 80gsm paper and brown envelope) and non-porous (tile and linoleum) substrates in a depletion series (30 depletions on non-porous and 20 on porous) and subjected to three ageing periods; 1, 7 and 28 days. A number of enhancement techniques were tested [fluorescence, black magnetic powder (BMP), iron-oxide black powder suspension (PS), cyanoacrylate (CA) fuming, acid violet 17 (AV17), acid yellow 7 (AY7), ninhydrin, DFO and Bluestar Forensic Magnum (BFM) luminol] to evaluate their potential effects on subsequent presumptive and confirmatory tests. AV17 and Bluestar provided the best enhancement and fully enhanced all depletions in the series. The sensitivity of the Kastle-Meyer (KM) (presumptive), Takayama and RSID-Blood tests (confirmatory) was initially investigated to determine the range of detectable depletions. The KM test detected all depletions, whereas the Takayama test detected up to depletion 6 and RSID-Blood detected up to depletion 20 (paper), 10 (envelope), 15 (tile) and 9 (lino). The abilities of these tests to detect blood after enhancement were then observed.A number of techniques resulted in little to no effect on any of the blood tests, whereas adverse effects were observed for others. Ninhydrin and CA fuming caused weak but instantaneous positive KM results whereas methanol-based AV17 and AY7 delayed the reaction by as much as 1 minute. The Takayama test was not very sensitive, therefore, its performance was easily affected by enhancement and negative results were often observed. RSID-Blood tests were largely unaffected by chemical enhancement although a drop in positive results was observed for some of the techniques when compared to positive controls.Using a standard procedure for DNA extraction, all the tested blood samples (before and after enhancement) gave a detectable quantity of DNA and were successfully profiled. Out of the 45 samples processed for DNA profiling, 44 gave full profiles, while the remaining showed allele drop out in one or two loci.

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Section: Accepted Manuscriptmentioning

confidence: 99%

The effect of mark enhancement techniques on the presumptive and confirmatory tests for blood

et al. 2018

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“…FPs have been adopted for physical sign identification of an individual in forensic science [1]. Nowadays, among several LFPs enhancement techniques, depending on the greater surface area of deposition and fluorescence abilities, the role of nanoscience and nanotechnology become an inevitable enhancing agent.…”

Section: Introductionmentioning

confidence: 99%

“…Detection of fingermarks by colloidal gold (MMD/SMD)-beyond the pH 3 limit. Forensic science international, 219(1)(2)(3),39-49. [11] Choi, M. J., McDonagh, A. M.,Maynard, P., nanoparticles and nano-structured particles in fingermark detection.…”

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confidence: 99%

Latent Fingerprint Enhancement Techniques: A Review

et al. 2020

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Fingerprint (FP) is a global mark used for personal identification. This study reviews recent latent fingerprint (LFP) enhancement techniques including metal oxides, multi-metal deposition (MMD-I/II/SMD/Au-ASP), optical, chemical, physical, and physicochemical. Furthermore, analytical techniques involved in identification, evaluation, and determination of pieces of evidence, and perpetrators including the SEM, TEM, UV-Vis, IR/NIR, SERS, SKP, DLS, MALDI-MSI, and TD analysis were also discussed. Among numerous LFP enhancement techniques, the application of chemical methods in combination with optical techniques has a greater place to recover FPs with sufficient quality. However, instead of using such a complex and costly enhancing agent, nowadays nanotechnology is using specific techniques used for visualizing, inspecting, gathering, and analyzing trace evidence at the scene of a crime. To indicate using simple metal oxides such as ZnO that have superior fluorescent properties and also that consider both the surface and cost of the materials, enhancement of the LFP is functioning.

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“…The results often showed that the possibility to obtain genetic profiles from touched items strictly depends on the surface type [12] in addition to the enhancement technique employed [13]. Plastic materials have often been employed in such studies with donor profiling information being successfully recovered [12,[14][15]. However, no studies have been conducted to investigate the deposition, recovery and profiling of touch DNA from cable sheaths after treatment with CNA and WPS.…”

Section: Introductionmentioning

confidence: 99%

Touch DNA—The prospect of DNA profiles from cables

et al. 2016

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Metal theft in the railroad industry poses significant challenges to transport investigators. Cable sheaths left behind at crime scenes, if appropriately analysed, could provide valuable evidence in a forensic investigation, but attempts at recovering DNA are not routinely made. Experiments were set up to ascertain the success in DNA recovery from the surface of cable sheaths after deposition of (a) sweat, (b) extracted DNA and (c) fingermarks. Since investigators try to collect fingermarks and often treat the cables with cyanoacrylate fuming (CNA fuming) or wet powder suspensions (WPS) to enhance the marks this study investigated the recovery of DNA from fingermarks pre- and post-enhancement. The double-swab technique and mini-taping were compared as options to recover DNA from the cable sheaths. Results demonstrate that generally, there is no significant difference between using swabs or mini-tapes to recover the DNA from the non-porous cables (p>0.05). It was also illustrated that CNA fuming performed better than WPS in terms of subsequent recovery and profiling of DNA. CNA fuming resulted in an average increase in DNA recovered via swabbing and taping (more than 4× and 8×, respectively), as compared to no treatment, with 50% of the DNA recovered after CNA fuming generating full DNA profiles.

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Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples

Cited by 12 publications

References 7 publications

The effect of mark enhancement techniques on the presumptive and confirmatory tests for blood

The effect of mark enhancement techniques on the presumptive and confirmatory tests for blood

Latent Fingerprint Enhancement Techniques: A Review

Touch DNA—The prospect of DNA profiles from cables

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