Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China

Dong, Xinyuan; Zhang, L.L; Wu, Zhijie; Li, D.P; Shang, Zifang; Gong, Ping

doi:10.4067/s0718-95162013005000046

Cited by 16 publications

(11 citation statements)

References 31 publications

(23 reference statements)

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“…Although the culturable methods only determine a minor percentage of microorganisms inhabiting the earthworm gut, the large decrease in the culturable denitrifying bacteria numbers might partly explain the inhibition of denitrification in the earthworm. This has been also confirmed by molecular technique by Dong et al (2013), who found that the copy numbers of the nirS gene in the DMPP-treated soils ranged from 34.5 to 45.5 % of that in the non-DMPP-treated soils after 49 days. The decreased richness of nirK gene in NI (DCD or DMPP)-treated soils (Table 4) might be due to the reduction of the abundance of denitrifying bacteria in the earthworm.…”

Section: Discussionsupporting

confidence: 66%

Nitrification inhibitors mitigate earthworm-induced N2O emission—a mesocosm study

Wang

Kong

et al. 2015

Biol Fertil Soils

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In this study, the soils with or without earthworms (Eisenia fetida) were treated with one of the two following nitrification inhibitors (NIs): dicyandiamide (DCD) or 3,4-dimethylpyrazole phosphate (DMPP); then, the soils were incubated for 90 days in laboratory mesocosms. The cumulative nitrous oxide (N 2 O) emission increased from 74.08 μg N 2 O-N kg −1 in the control to 384.69 μg N 2 O-N kg −1 in the earthworm-treated soil. The addition of NI decreased the cumulative N 2 O emissions from soils by 67.1 % in the DCD treatment and 73.4 % in the DMPP treatment without earthworms and by 79.0 and 81.0 % with earthworms, respectively. After 90 days, the richness of nirK gene in the soil decreased in both DCD and DMPP treatments compared with control, and the abundance of denitrifying bacteria in the gut of earthworm declined to less than 1/1000 or 1/100 of those without NIs. At 7 and 90 days, N 2 O production by denitrification in earthworm gut homogenates in both NI treatments decreased compared to those without NIs. Therefore, this study provided a fundamental evidence for that the application of NImitigated N 2 O emission induced by earthworm (E. fetida).

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Section: Discussionsupporting

confidence: 66%

Nitrification inhibitors mitigate earthworm-induced N2O emission—a mesocosm study

Wang

Kong

et al. 2015

Biol Fertil Soils

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“…This finding was further supported by a series of laboratory microcosms of six pasture soils (15). In contrast, other laboratory incubation studies have found that DMPP application significantly decreased the abundance and transcriptional activity of both AOA and AOB (35,36). Recently, a change in the community structure of AOB in response to DMPP application was observed (37).…”

mentioning

confidence: 51%

Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate on Nitrification and Nitrifiers in Two Contrasting Agricultural Soils

Shi

Müller

et al. 2016

Appl Environ Microbiol

109

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The nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) is a powerful tool that can be used to promote nitrogen (N) use efficiency and reduce N losses from agricultural systems by slowing nitrification. Mounting evidence has confirmed the functional importance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in nitrification and N 2 O production; however, their responses to DMPP amendment and the microbial mechanisms underlying the variable efficiencies of DMPP across different soils remain largely unknown. Here we compared the impacts of DMPP on nitrification and the dynamics of ammonia oxidizers between an acidic pasture soil and an alkaline vegetable soil using a 15 N tracing and 13 CO 2 -DNA-stableisotope probing (SIP) technique. The results showed that DMPP significantly inhibited nitrification and N 2 O production in the vegetable soil only, and the transient inhibition was coupled with a significant decrease in AOB abundance. No significant effects on the community structure of ammonia oxidizers or the abundances of total bacteria and denitrifiers were observed in either soil. The 15 N tracing experiment revealed that autotrophic nitrification was the predominant form of nitrification in both soils. The 13 CO 2 -DNA-SIP results indicated the involvement of AOB in active nitrification in both soils, but DMPP inhibited the assimilation of 13 CO 2 into AOB only in the vegetable soil. Our findings provide evidence that DMPP could effectively inhibit nitrification through impeding the abundance and metabolic activity of AOB in the alkaline vegetable soil but not in the acidic pasture soil, possibly due to the low AOB abundance or the adsorption of DMPP by organic matter. IMPORTANCEThe combination of the 15 N tracing model and 13 CO 2 -DNA-SIP technique provides important evidence that the nitrification inhibitor DMPP could effectively inhibit nitrification and nitrous oxide emission in an alkaline soil through influencing the abundance and metabolic activity of AOB. In contrast, DMPP amendment has no significant effect on nitrification or nitrifiers in an acidic soil, potentially owing to the low abundance of AOB and the possible adsorption of DMPP by organic matter. Our findings have direct implications for improved agricultural practices through utilizing the nitrification inhibitor DMPP in appropriate situations, and they emphasize the importance of microbial communities to the efficacy of DMPP.

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“…For the AOA there was not even a trend; they remained unaffected. Di and Cameron (2011) and Dong et al (2013) also reported a significant reduction of the AOB after addition of DMPP. Further results of a lower response of AOA for NIs are summarized in Table 2.…”

Section: Effect Of Nis On Soil Microbesmentioning

confidence: 89%

The effect of nitrification inhibitors on the nitrous oxide (N₂O) release from agricultural soils—a review

Ruser

2015

J. Plant Nutr. Soil Sci.

336

205

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The use of nitrification inhibitors (NI) is a technique which is able to improve N fertilizer use efficiency, to reduce nitrate leaching and to decrease the emission of the climate-relevant gas N 2 O simultaneously, particularly in moderately fertilized agricultural systems adapted to plant N demand. The ammonia monooxygenase (AMO) is the first enzyme which is involved in the oxidation of NH þ 4 to NO À 3 in soils. The inhibition of the AMO by NIs directly decreases the nitrification rate and it reduces the NO À 3 concentration which serves as substrate for denitrification. Hence, the two main pathways of N 2 O production in soils are blocked or their source strength is at least decreased. Although it has been shown that archaea are also able to oxidize NH 3 , results from literature suggest that the enzymatic activity of NH 3 oxidizing bacteria is the most important target for NIs because it was much stronger affected. The application of NIs to reduce N 2 O emissions is most effective under conditions in which the NI remains close to the N-fertilizer. This is the case when the NI was sprayed on mineral-N fertilizer granules or thoroughly mixed with liquid fertilizers. Most serious problems of spatial separation of NI and substrate emerge on pasture soils, where N 2 O hotspots occur under urine and to a lesser extent under manure patches. From the few studies on the effect of different NI quantities it seems that the amount of NI necessary to reduce N 2 O emissions is below the recommendations for NI amounts in practice. NIs can improve the fertilizer value of liquid manure. For instance, the addition of NIs to slurry can increase N uptake and yield of crops when NO À 3 -N leaching losses are reduced. It has clearly been demonstrated that NIs added to cattle slurry are very effective in reducing N 2 O as well as NO emissions after surface application and injection of slurry into grassland soils. In flooded rice systems NIs can reduce CH 4 emission significantly, whereas the effect on CO 2 emission is varying. On the other hand, as an effect of the delay of nitrification by NIs, NH 3 emission might increase when N fertilizers are not incorporated into the soil. As compared to other measures NIs have a high potential to reduce N 2 O emissions from agricultural soils. Further, no other measure has so consistently been proofed according its efficiency to reduce N 2 O emissions. From the published data [Akiyama et al. (2010) and more recent data from the years 2010-2013; 140 data sets in total] a reduction potential of approx. 35% seems realistic; however, further measurements in different management systems, particularly in regions with intense frost/thaw cycles seem necessary to confirm this reduction potential. These measurements generally should cover a whole annual cycle.

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Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China

Cited by 16 publications

References 31 publications

Nitrification inhibitors mitigate earthworm-induced N2O emission—a mesocosm study

Nitrification inhibitors mitigate earthworm-induced N2O emission—a mesocosm study

Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate on Nitrification and Nitrifiers in Two Contrasting Agricultural Soils

The effect of nitrification inhibitors on the nitrous oxide (N₂O) release from agricultural soils—a review

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Effects of the nitrification inhibitor DMPP on soil bacterial community in a Cambisol in northeast China

Cited by 16 publications

References 31 publications

Nitrification inhibitors mitigate earthworm-induced N2O emission—a mesocosm study

Nitrification inhibitors mitigate earthworm-induced N2O emission—a mesocosm study

Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate on Nitrification and Nitrifiers in Two Contrasting Agricultural Soils

The effect of nitrification inhibitors on the nitrous oxide (N2O) release from agricultural soils—a review

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The effect of nitrification inhibitors on the nitrous oxide (N₂O) release from agricultural soils—a review