Effects of the pharmaceutical cosolvent hydroxypropyl-β-cyclodextrin on porcine corneal endothelium

Duncker, G.; Reichelt, Jan

doi:10.1007/s004170050094

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…Duckner et al studied the effect of HPβCD concentration on porcine corneal endothelium (12). It was observed that use of 10%w/v HPβCD caused high grade of endothelial damage followed by minimal damage with 1%w/v HPβCD and none with 0.1%w/v HPβCD within 3 h of the study.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas

2015

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Abstract. The objective of the present study is to investigate the confounding effects, if any, of betacyclodextrins (βCDs) on corneal permeability coefficients obtained from in vitro transmembrane diffusion studies. Transcorneal permeability studies were carried out with 2-hydroxypropyl-beta-cyclodextrin (HPβCD) and randomly methylated-beta-cyclodextrin (RMβCD) at 5 and 2.5%w/v in isotonic phosphate-buffered solution (IPBS) (pH 7.4). Rabbit corneas received from Pel-Freez Biologicals® were used for these studies. Propranolol hydrochloride (PHCl) (1 mg/mL) was used as the paracellular permeability marker. A series of permeation studies were carried out with IPBS as the control, with CDs on the donor side only, CDs on the receiver side only, and CDs on both the donor and receiver sides. At the end of 1 or 3 h, corneas were collected and fixed using a solution containing 2%v/v glutaraldehyde+2%w/v paraformaldehyde+IPBS and histological examinations were performed (Excalibur Pathology, Inc). The order of transcorneal permeability of PHCl was found to be CDs on the receiver side > control (no CDs) ≈ CDs on both the receiver and donor sides > CDs on the donor side. Histology studies revealed that the corneal epithelial and endothelial layers remained intact in the control sets. Damage to the cornea was observed in the order of CDs on the receiver side > CDs on the donor side > CDs on both sides > control. The use of CDs in solutions for in vitro permeation experiments with rabbit corneas needs to be carefully considered to avoid confounding effects in the data obtained.

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Section: Discussionmentioning

confidence: 99%

Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas

2015

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Einfluss von Cyclodextrinen auf die okulare Verfügbarkeit von Antiglaukomatosa und Immunsuppressiva in vitro / in vivo

Knapp-Ulrich¹

2004

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Effects of the pharmaceutical cosolvent hydroxypropyl-β-cyclodextrin on porcine corneal endothelium

Abstract: Severe endothelial destruction in 10% HPBCD and changes in membrane integrity at lower concentrations limit the use of HPBCD in ophthalmic solutions.

Cited by 2 publications

References 24 publications

Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas

Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas

Einfluss von Cyclodextrinen auf die okulare Verfügbarkeit von Antiglaukomatosa und Immunsuppressiva in vitro / in vivo

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