Effects of the plant growth regulator abscisic acid and high osmoticum on the developmental expression of the maize catalase genes

Guan, Lingqiang; Scandalios, John G.

doi:10.1034/j.1399-3054.1998.1040317.x

Cited by 46 publications

(51 citation statements)

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“…To cope with oxidative stress, plants have evolved several enzymatic and non-enzymatic systems. The channels by which H 2 O 2 mediates the induction of stomata closure, is by inducing the expression of antioxidant genes SOD (Sakamoto et al 1995;Guan and Scandalios 1998a;Bueno et al 1998), and CAT (Guan and Scandalios 1998b;Guan et al 2000), and increase the activities of antioxidative enzymes such as SOD, CAT, guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR) in plant tissues (Prasad et al 1994;Gong et al 1998;Bellaire et al 2000). SOD reduces superoxide radicals (O 2 -) to H 2 O 2 , CAT reduces H 2 O 2 to water and dioxygen, APX reduces H 2 O 2 to water and monodehydroascorbate, thus preventing the formation of the highly reactive hydroxyl radical (OH) that can cause lipid peroxidation, protein denaturation, and DNA mutations.…”

Section: Discussionmentioning

confidence: 99%

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

Zhang

Han

Yang

et al. 2009

Plant Cell Tiss Organ Cult

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Hydrogen peroxide (H 2 O 2 ) content and transcript levels of genes encoding superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) antioxidant enzymes were investigated during different stages of somatic embryogenesis in Larix leptolepis. H 2 O 2 content was lowest on day 0 when embryogenic callus was incubated on Murashige and Skoog (MS) medium supplemented with polyethylene glycol (PEG). This content began to increase when callus was transferred to the same medium but containing abscisic acid (ABA), and reached a peak at day 3 following incubation. The level of H 2 O 2 dropped from day 7 to day 10, peaked at day 21, then dropped again at day 24 and day 35, but increased when somatic embryos reached maturity at day 45. Transcript levels of SOD, CAT, and APX were lowest when somatic embryos were cultured on callus induction medium without ABA. When calli were transferred onto somatic embryo maturation medium, expression patterns of SOD, CAT, and APX varied, and transcript levels at all stages were higher than those at d 0. SOD expression was highest at day 3. Whereas, CAT expression levels were low during early stages of somatic embryogenesis, but increased at day 21, declined at days 24 and 35, and then began to increase again at day 45. APX gene expression patterns were highest at days 3, 21, and 45. These results suggested that ABA was essential for promoting somatic embryogenesis of L. leptolepis. Moreover, ABA induced production of H 2 O 2 and other active oxygen species (AOS), and mediated CAT, SOD, and APX gene expression in somatic embryogenesis of L. leptolepis.

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Section: Discussionmentioning

confidence: 99%

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

Zhang

Han

Yang

et al. 2009

Plant Cell Tiss Organ Cult

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“…It has been shown that ABA can cause an increased generation of H 2 O 2 (Guan et al 2000, Pei et al 2000, induce the expression of antioxidant genes encoding Cu, Zn-superoxide dismutase (Cu, Zn-SOD) (Sakamoto et al 1995, Guan and Scandalios 1998a, Mn-SOD (Zhu and Scandalios 1994, Bueno et al 1998 and Fe-SOD , and catalase (CAT) (Williamson and Scandalios 1992, Anderson et al 1994, Guan and Scandalios 1998b, Guan et al 2000, and increase the activities of antioxidative enzymes such as SOD, CAT, guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR) in plant tissues (Anderson et al 1994, Prasad et al 1994a, Bueno et al 1998, Gong et al 1998, Bellaire et al 2000. In addition, ABA can cause the increase of lipid peroxidation expressed as malondialdehyde (MDA) production in plant cells (Bueno et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Effect of Abscisic Acid on Active Oxygen Species, Antioxidative Defence System and Oxidative Damage in Leaves of Maize Seedlings

Jiang

Zhang

2001

Plant and Cell Physiology

846

468

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;Leaves of maize (Zea mays L.) seedlings were supplied with different concentrations of abscisic acid (ABA). Its effects on the levels of superoxide radical (O 2 -), hydrogen peroxide (H 2 O 2 ) and the content of catalytic Fe, the activities of several antioxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR), the contents of several non-enzymatic antioxidants such as ascorbate (ASC), reduced glutathione (GSH), a a a a-tocopherol (a a a a-TOC) and carotenoid (CAR), and the degrees of the oxidative damage to the membrane lipids and proteins were examined. Treatment with 10 and 100 m m m mM ABA significantly increased the levels of O 2 -and H 2 O 2 , followed by an increase in activities of SOD, CAT, APX and GR, and the contents of ASC, GSH, a a a a-TOC and CAR in a dose-and time-dependent pattern in leaves of maize seedlings. An oxidative damage expressed as lipid peroxidation, protein oxidation, and plasma membrane leakage did not occur except for a slight increase with 100 m m m mM ABA treatment for 24 h. Treatment with 1,000 m m m mM ABA led to a more abundant generation of O 2 -and H 2 O 2 and a significant increase in the content of catalytic Fe, which is critical for H 2 O 2 -dependent hydroxyl radical production. The activities of these antioxidative enzymes and the contents of a a a a-TOC and CAR were still maintained at a higher level, but no longer further enhanced when compared with the treatment of 100 m m m mM ABA. The contents of ASC and GSH had no changes in leaves treated with 1,000 m m m mM ABA. These results indicate that treatment with low concentrations of ABA (10 to 100 m m m mM) induced an antioxidative defence response against oxidative damage, but a high concentration of ABA (1,000 m m m mM) induced an excessive generation of AOS and led to an oxidative damage in plant cells.

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“…Only a slight activation could be seen for ABA-treated coleoptiles. Guan and Scandalios (1998) found that Sod 4A transcript was increased under the influence of ABA in young maize leaves. They stated that ABA mediated changes in oxygen free radical levels activated antioxidant enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…These treatments cause a wide variety of morphological, physiological and biochemical effects. On the molecular level they are manifested as changes in the pattern of gene expression (Skriver and Mundy 1990, Guan and Scandalios 1998, Guan et al 2000. Recent investigations showed that CHF frequently retarded growth and caused yield losses at crop harvest (Fletcher et al 1995, Rengel andWheal 1997).…”

mentioning

confidence: 99%

The effect of plant growth regulators and chlorsulfuron on electrophoretic profiles of soluble proteins, polypeptides and antioxidant enzymes in maize seedlings

Nedeva¹,

Petkova²,

Angelova³

et al. 2005

PLANT SOIL ENVIRON

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Investigations were carried out with maize (Zea mays L.) seedlings. The effect of chlorsulfuron (CHF) on the electrophoretic profiles of the total and thermostable proteins, polypeptides and some antioxidant enzymes was compared to the effect of abscisic acid (ABA), benzylaminopurine (BAP) and N 1 -(2-chloro-4-pyridyl)-N 2 -phenylurea (4PU-30) applied in concentrations that inhibit the growth of seedlings. It was established that the effect of CHF on seedling growth as well as on the studied biochemical parameters was most significant. The reduction of growth by CHF was accompanied by a significant decrease in the quantity of main proteins and specific qualitative changes in the electrophoretic pa�erns for coleoptiles. The appearance of a new native protein with Rm 0.22 and polypeptide with MW 67 kDa was demonstrated. Some similarity of the effect on protein band with Rm value 0.22 was observed under the influence of 4PU-30 as well. In the endosperm the effect of CHF was manifested as a delay of degradation. CHF and 4PU-30 influenced the activity of basic peroxidases in the opposite manner. The strong inhibiting effect of both cytokinins BAP and 4PU-30 on the activity of slow migrating superoxide dismutase (SOD) isoenzymes was observed. Each of the tested substances shows a differential effect on the proteins and antioxidant enzymes, despite the fact that all of them were applied at concentrations that inhibit the growth of seedlings.

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Effects of the plant growth regulator abscisic acid and high osmoticum on the developmental expression of the maize catalase genes

Cited by 46 publications

References 0 publications

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

Effect of Abscisic Acid on Active Oxygen Species, Antioxidative Defence System and Oxidative Damage in Leaves of Maize Seedlings

The effect of plant growth regulators and chlorsulfuron on electrophoretic profiles of soluble proteins, polypeptides and antioxidant enzymes in maize seedlings

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