Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells

Wang, Qun; Liu, Fei; Chen, Xuexiang; Yang, Zhijie; Cao, Yong

doi:10.1016/j.ijbiomac.2020.06.203

Cited by 22 publications

(13 citation statements)

References 35 publications

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“…The three absorption bands, i.e., 1150, 1110, and 1042 cm -1 , in the 1000-1200 cm -1 range demonstrated the presence of pyranose ring in the CPs-1 [39]. Moreover, the feature peaks at approximately 920 and 851 cm -1 were associated with βand α-D-galactopyranose, respectively [40,41]. The absorption peak at 761.6 cm -1 was correlated with α-D-xylopyranose [42].…”

Section: Ft-ir Analysis Of Cps-1mentioning

confidence: 91%

Novel Compound Polysaccharides from Chinese Herbal Medicines: Purification, Characterization, and Antioxidant Activities

Yang

Feng

et al. 2022

Oxidative Medicine and Cellular Longevity

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The present study investigated physicochemical properties and antioxidant activities in vivo and in vitro of purified compound polysaccharides (CPs-1) from Chinese herbal medicines, composed of lotus leaf, hawthorn, Fagopyrum tataricum, Lycium barbarum, Semen cassiae, and Poria cocos with the mass ratio of 2 : 4 : 2 : 1 : 1.5 : 1. The HPGPC profile and FT-IR spectra indicated that the average molecular weight of CPs-1 was 38.7 kDa and possessed the α- and β-D-pyranose, respectively. The methylation analysis and NMR spectrum demonstrated that CPs-1 had a →6)-β-D-Glcp-(1→6)-β-D-Glcp(1→ backbone. Furthermore, the antioxidant assays in vitro revealed that CPs-1 displayed high scavenging abilities for DPPH, hydroxyl, and reducing power, as well as ABTS and superoxide scavenging capacity. The antioxidant experiments in vivo revealed that CPs-1 could significantly enhance CAT, SOD, and GSH-Px activities and dramatically reduce MDA levels in liver and serum of high-fat mice. Therefore, CPs-1 could be potentially incorporated into pharmaceutical products or functional foods as a natural antioxidant.

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Section: Ft-ir Analysis Of Cps-1mentioning

confidence: 91%

Novel Compound Polysaccharides from Chinese Herbal Medicines: Purification, Characterization, and Antioxidant Activities

Yang

Feng

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…These changes, always closely related to the damage of cell microfilaments and TJs, are characterized by the transepithelial electrical resistance (TEER) value and label permeability of the Caco-2 monolayer. , The anti-inflammatory activity and regulation of TJ expression are key approaches for food factors to repair the intestinal barrier. Many active polysaccharides, such as Plantago asiatica polysaccharide, Lycium barbarum polysaccharide, Spirulina polysaccharide, and Coix lachryma-jobi total polysaccharide, have been shown to inhibit the expression of pro-inflammatory cytokines (TNF-α, IL-8, IL-6, IL-1β, and IL-12) in Caco-2 cells and regulate the expression of TJs (occludin, claudin-1, ZO-1, and ZO-3) to improve epithelial barrier dysfunction in the Caco-2 cell monolayer models.…”

Section: Protective Effect Of Polysaccharides On Intestinal Barrier F...mentioning

confidence: 99%

“…It is reported that polysaccharides with intestinal barrier protection have higher MWs. For example, polysaccharides from Spirulina, G. atrum, and longan pulp with MWs of 623.0, 1013.0, and 159.3 kDa, respectively, exerted strong intestinal barrier protective effects.…”

Section: Protective Effect Of Polysaccharides On Intestinal Barrier F...mentioning

confidence: 99%

Protective Effects of Natural Polysaccharides on Intestinal Barrier Injury: A Review

Huo

Sun

et al. 2022

J. Agric. Food Chem.

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Owing to their minimal side effects and effective protection from oxidative stress, inflammation, and malignant growth, natural polysaccharides (NPs) are a potential adjuvant therapy for several diseases caused by intestinal barrier injury (IBI). More studies are accumulating on the protective effects of NPs with respect to IBI, but the underlying mechanisms remain unclear. Thus, this review aims to represent current studies that investigate the protective effects of NPs on IBI by directly maintaining intestinal epithelial barrier integrity (inhibiting oxidative stress, regulating inflammatory cytokine expression, and increasing tight junction protein expression) and indirectly regulating intestinal immunity and microbiota. Furthermore, the mechanisms underlying IBI development are briefly introduced, and the structure–activity relationships of polysaccharides with intestinal barrier protection effects are discussed. Potential developments and challenges associated with NPs exhibiting protective effects against IBI have also been highlighted to guide the application of NPs in the treatment of intestinal diseases caused by IBI.

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“…The C57BL/6 mice (7 weeks, male) weighing 20-22 g were obtained from the Laboratory Animal Centre of the Army Medical University (Chongqing, China) and housed in a controlled environment (22)(23)(24)(25) • C, 50-55%). They were provided standard food (D12450B; 10% fat, 70% carbohydrate, 20% protein) and obtained water freely (29).…”

Section: Experimental Animals and Designmentioning

confidence: 99%

“…Given the crucial role of the practical application for athletes, dietary approaches are preferentially recommended to prevent intestinal barrier loss ( 21 ). In this sense, natural bioactive substances such as plant polyphenols are emerging as sports nutrition supplements for GIS prevention ( 22 , 23 ). Research studies have recently indicated that a Mediterranean diet containing a high proportion of polyphenolic compounds ( 24 ) could significantly prevent intestinal barrier dysfunction ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise

et al. 2022

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Mounting evidence suggested that high loading intensity of exercise might be detrimental to human health, especially the gastrointestinal tract. Pterostilbene (PTE), derived from grapes and blueberries, might reach a high concentration of intestinal contents. Our study aimed to evaluate PTE’s ability to prevent the loss of intestinal epithelial barrier in high loading intensity of exercise. The exercise model was established by the forced running of mice. An effective HPLC-UV method was developed to quantify PTE concentration in intestinal content. The mRNA changes were detected by quantitative polymerase chain reaction (qPCR). The structure of intestinal flora was analyzed by 16S rRNA sequencing. The PTE (100 mg/kg/d) could significantly attenuate exercise-induced intestinal epithelial barrier loss. Moreover, the HPLC-UV assay showed that the PTE concentration of intestinal content could last 12 h. Furthermore, the exercise increased the abundance of Alistipes, which was related to lipopolysaccharide (LPS) production but could not be reversed by PTE intervention. Besides, cell experiments showed that PTE could promote the expression of intestinal epithelial tight junction (TJ) molecules in vitro. In conclusion, PTE has a significant interest in preventing exercise-induced intestinal damage.

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Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells

Cited by 22 publications

References 35 publications

Novel Compound Polysaccharides from Chinese Herbal Medicines: Purification, Characterization, and Antioxidant Activities

Novel Compound Polysaccharides from Chinese Herbal Medicines: Purification, Characterization, and Antioxidant Activities

Protective Effects of Natural Polysaccharides on Intestinal Barrier Injury: A Review

Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise

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