Effects of the protein phosphorylation inhibitor genistein on maturation of pig oocytes in vitro

Jung, Thomas; Fulka, J.; Lee, C.; Moor, R. M.

doi:10.1530/jrf.0.0980529

Cited by 28 publications

(15 citation statements)

References 15 publications

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“…However, a significantly lower percentage of oocytes reached the MI stage (62%) compared to simple 24-hour cultivation without GEN in the medium (95%). 32% of the oocytes showed abnormal The results of the experiment are in accordance with data reported by Jung et al (1993) as regards the effect observed -the inhibition of the germinal vesicle breakdown (GVBD) and also the assessment of the highest effective concentration of GEN in the medium. Makarevich et al (1997), however, reported a stimulating effect of low dosage of GEN (5 µg/ml) on pig oocyte maturation, but this was probably due to reserves of the culture system used -after 40 hours of cultivation only 58% of the control oocytes reached any corresponding stage of nuclear maturation (AI-MII) and 20% of oocytes did not enter the maturation process at all.…”

Section: Resultssupporting

confidence: 89%

“…Makarevich et al (1997), however, reported a stimulating effect of low dosage of GEN (5 µg/ml) on pig oocyte maturation, but this was probably due to reserves of the culture system used -after 40 hours of cultivation only 58% of the control oocytes reached any corresponding stage of nuclear maturation (AI-MII) and 20% of oocytes did not enter the maturation process at all. Although Jung et al (1993), after reversing 24-hour cultivation, did not determine any effects similar to ours, they also noticed deviations from standards after previous GEN exposure. They reported significantly impaired maturation from MI to MII stages.…”

Section: Resultssupporting

confidence: 67%

“…The authors of the above-mentioned studies connect the observed effects precisely with TPK inhibition for the most part. Jung et al (1993) demonstrated the inhibition of protein phosphorylation in the affected oocytes. Moore and Kinsey (1994) observed comparable effects of GEN with those of erbstatin and lavendustin A, other TPK inhibitors, in sea urchin oocytes.…”

mentioning

confidence: 94%

“…GEN is capable of significantly influencing the in vitro maturation of mammalian oocytes. Depending on the dosage, it blocks the germinal vesicle breakdown (GVBD) in mouse (Makarevich et al, 1997) and pig oocytes (Jung et al, 1993). Tatemoto and Terada (1996) did not find any significant inhibition of GVBD in cattle oocytes cultivated with GEN, but depending on the dosage, there was an increase in the number of oocytes that blocked meiotic maturation in the stages of late diakinesis (LD) or premetaphase I (PMI).…”

mentioning

confidence: 99%

“…Moore and Kinsey (1994) observed comparable effects of GEN with those of erbstatin and lavendustin A, other TPK inhibitors, in sea urchin oocytes. The effects on nuclear maturation are explained by the resulting inhibition of MAP kinase (Jung et al, 1993;Sun et al, 1998) and directly of p34 cdc2 kinase (Tatemoto and Terada, 1996). The suppression of cumular expansion might be connected with the blocking of the function of receptors for the epidermal growth factor -EGF and partially the follicle stimulating hormone -FSH (Tirone et al, 1997;Ježová et al, 2001;Procházka et al, 2003).…”

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confidence: 99%

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Effects of genistein and genistin on in vitro maturation of pig oocytes

Vodková¹,

Rajmon²,

Petr³

et al. 2008

CZECH J ANIM SCI

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ABSTRACT:The objective of the study was to verify the hypothesis that GEN (genistein -phytoestrogen and an inhibitor of tyrosine protein kinase -TPK) effects on pig oocyte maturation and cumular cell expansion under in vitro conditions are connected with its estrogenic activity. Oocytes were cultivated for 24 hours up to the stage of the first meiotic metaphase (MI). Three different doses of GEN (13, 40, 80 µg/ml of medium) and also three doses of GIN, genistin, an analogue of GEN without effects on TPK, (80, 160 and 240 µg/ml of medium) were tested. To verify the reversibility of GEN effects, the oocytes were first cultivated for 24 hours with 80 µg of GEN per 1 ml of medium and then for another 24 hours without any GEN. GEN blocked pig oocyte maturation at the stage of the germinal vesicle (GV), depending on the dose. After rinsing out the GEN the oocyte maturation recovered, but with abnormalities (32%). GIN in a concentration of 80 µg/ml of medium induced a significant blockage at the GV stage (18%). With an increase in the GIN concentration, the number of oocytes blocked at the GV stage significantly decreased, but the abnormal maturation increased (up to 31%). GEN inhibited the cumular cell expansion in proportion to its dose. GIN had a less pronounced effect. As GEN and GIN effects demonstrate similar patterns, it is probable that estrogenic activity is involved.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 67%

mentioning

confidence: 94%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of genistein and genistin on in vitro maturation of pig oocytes

Vodková¹,

Rajmon²,

Petr³

et al. 2008

CZECH J ANIM SCI

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The thiol reagent, thimerosal, irreversibly inhibits meiosis reinitiation in mouse oocyte when applied during a very early and narrow temporal window: A pharmacological analysis

Alexandre

Delsinne

Goval

2003

Molecular Reproduction Devel

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The effect of the sulfhydryl reagent, thimerosal (TMS) on meiosis resumption in germinal vesicle (GV)-stage denuded mouse oocytes was studied. It irreversibly inhibits both GV breakdown (GVBD) and the first polar body (pb1) extrusion in concentration- and time-dependent manners, the most striking result being the very early and narrow temporal window during which denuded primary oocytes released from their follicle are susceptible to a pulse of the drug. This inhibition is bypassed by dithiothreitol (DTT) with an efficiency declining with time, while thiosalicylic acid (TA), an analog of TMS devoid of the mercury atom, has no effect on meiosis reinitiation. These results strongly suggest that the inhibitory effect of TMS is a consequence of its sulfhydryl group oxidising activity. The molecular target(s) of this inhibitory oxidation should however be identified. In contrast to DTT, okadaic acid (OA), known to bypass the inhibitory effect of drugs interfering with protein kinase activities, only induces chromatin condensation and GVBD in TMS-pulsed oocytes with a delay of about 8 hr as compared to the control situation. This confirms that a very early thiol oxidation induced by TMS exerts a much more dramatic effect on resumption on meiosis than any pharmacological manipulation of protein kinase activities leading to activation of MPF.

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Effects of stimulators of protein kinases A and C and modulators of phosphorylation on plasminogen activator activity in porcine oocyte‐cumulus cell complexes during in vitro maturation

Kim

Menino

1995

Molecular Reproduction Devel

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Effects of phorbol myristate acetate (PMA), dibutyryl cyclic AMP (dbcAMP), 6-dimethylaminopurine (6-DMAP), and okadaic acid (OA) on plasminogen activator (PA) activity in porcine oocyte-cumulus cell complexes (POCC) in vitro were determined. Cumulus cell-enclosed oocytes were collected from 1-4 mm antral follicles and cultured in TCM-199 with 0.3% polyvinylpyrrolidone for 48 hr. PA activities in POCC were quantified using SDS-PAGE, casein-agar zymography, and densitometry. Two plasminogen-dependent lytic zones (93-96 kD and 71-79 kD) were observed in POCC. Addition of amiloride to the zymography, a competitive inhibitor of urokinase-type PA, failed to reduce activities in either zone, suggesting that the 71-79 kD band is a tissue-type PA (tPA) and the 93-96 kD band is possibly a tPA-inhibitor complex. Changes in PA activity due to the various treatments were expressed relative to the PA activity in 40 POCC. Increasing dbcAMP increased PA (P < 0.05) activity in dose-dependent fashion, whereas 6-DMAP and 10 and 100 ng/ml PMA inhibited (P < 0.05) PA activity. PA activity increased (P < 0.05) in POCC treated with up to 25 nM OA; however, activity decreased (P < 0.05) at concentrations > 75 nM. Treatment with 25 nM OA also induced the expression of an amiloride-sensitive PA (49-52 kD). Germinal vesicle breakdown and progression to metaphase II were inhibited (P < 0.05) by 2.5 mM dbcAMP and 2 mM 6-DMAP, whereas 100 ng/ml PMA and 25 nM OA inhibited (P < 0.05) only progression to metaphase II.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effects of the protein phosphorylation inhibitor genistein on maturation of pig oocytes in vitro

Cited by 28 publications

References 15 publications

Effects of genistein and genistin on in vitro maturation of pig oocytes

Effects of genistein and genistin on in vitro maturation of pig oocytes

The thiol reagent, thimerosal, irreversibly inhibits meiosis reinitiation in mouse oocyte when applied during a very early and narrow temporal window: A pharmacological analysis

Effects of stimulators of protein kinases A and C and modulators of phosphorylation on plasminogen activator activity in porcine oocyte‐cumulus cell complexes during in vitro maturation

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