Effects of the putative neutrophil-generated toxin, hypochlorous acid, on membrane permeability and transport systems of Escherichia coli.

Albrich, J M; Gilbaugh, J H; Callahan, K B; Hurst, James K.

doi:10.1172/jci112548

Cited by 87 publications

(49 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The interaction of peroxidases with H 2 O 2 forms hypohalous acids, which are potent oxidants known to have several cytotoxic effects on mammalian and bacterial cells. Cell membrane integrity may be violated by membrane peroxidation and the oxidation and/or decarboxylation of membrane proteins [47,48]. In the current study, serum peroxidases significantly increased in D. puntazzo exposed to E. leei at all sampling points with the highest stimulation at 40 days p.e.…”

Section: Discussionsupporting

confidence: 45%

Sharpsnout sea bream (Diplodus puntazzo) humoral immune response against the parasite Enteromyxum leei (Myxozoa)

Muñoz

Cuesta

Athanassopoulou

et al. 2007

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 45%

Sharpsnout sea bream (Diplodus puntazzo) humoral immune response against the parasite Enteromyxum leei (Myxozoa)

Muñoz

Cuesta

Athanassopoulou

et al. 2007

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…2), suggesting that E. coli cells are able to maintain membrane permeability after chlorination. These results agree with the conclusions reached by Albrich et al (1986) who concluded that the bactericidal effect of hypochlorous acid on E. coli is not linked to changes in membrane permeability.…”

Section: ------------------------------------------------------------supporting

confidence: 93%

Chlorination and ozonation of waste‐water: comparative analysis of efficacy through the effect onEscherichia colimembranes

Arana

Santorum

Muela

et al. 1999

Journal of Applied Microbiology

View full text Add to dashboard Cite

I . A RA N A, P. S AN TO R UM , A . MU EL A AN D I . BA RC I NA . 1999. The effect of chlorine and ozone on Escherichia coli cells resuspended in waste-water was compared. Selected chlorination and ozonation conditions produced a similar decrease in culturability (2-2·5 log). Under these conditions, differences in membrane permeability and cell surface hydrophobicity, depending on the disinfectant tested, were detected. After ozonation, while no changes in cell surface hydrophobicity were observed, approximately 95·5% of cells showed altered membrane permeability. The effect of chlorine was not linked to changes in membrane permeability. After chlorination, E. coli cells showed a tendancy to aggregate. The possibility that aggregation of cells could interfere with conventional colony counts is discussed. The degree of toxicity (Microtox TM assay) was unrelated to the effect on cellular activity.

show abstract

“…To date, the antimicrobial activity of AEW has been thought to derive from hypochlorous acid (HClO), which has a low pH and a high oxidation-reduction potential (ORP) (Al-Haq et al, 2005). Some studies have also suggested that HClO, as an undissociated form of chlorine, penetrates microbial cell membranes and subsequently achieves its antimicrobial action through the oxidation of key metabolic enzymes (Albrich et al, 1986;Barrette et al, 1989;Hurst et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Involvement of reactive oxygen species in the cytotoxic effect of acid-electrolyzed water

2015

View full text Add to dashboard Cite

-Acid-electrolyzed water (AEW) is commonly used as a disinfectant in the agricultural and medical fields. Although several studies have been conducted to examine its toxicity in vitro and in vivo, the cytotoxic mechanism of AEW has never been verified. The purpose of the present study was to elucidate the underlying mechanism by which AEW exerts its in vitro cytotoxic effect. Mouse fibroblasts treated with AEW experienced dilution rate-dependent cytotoxic effects in the 100% confluent phase as well as in the mitotic phase. The levels of intracellular reactive oxygen species (ROS) increased significantly in fully-confluent cells treated with undiluted and four times diluted AEW. In both of these treatments, cytotoxicity was also observed. It is thus concluded that the in vitro cytotoxicity of AEW is attributable to increased intracellular ROS. Additionally, the ROS responsible for these effects appears to be, at least in part, hydroxyl radical because the increase in intracellular ROS was attenuated by post-treatment with dimethyl sulfoxide, a hydroxyl radical scavenger, and with the antioxidant polyphenol, proanthocyanidin.

show abstract

Effects of the putative neutrophil-generated toxin, hypochlorous acid, on membrane permeability and transport systems of Escherichia coli.

Cited by 87 publications

References 33 publications

Sharpsnout sea bream (Diplodus puntazzo) humoral immune response against the parasite Enteromyxum leei (Myxozoa)

Sharpsnout sea bream (Diplodus puntazzo) humoral immune response against the parasite Enteromyxum leei (Myxozoa)

Chlorination and ozonation of waste‐water: comparative analysis of efficacy through the effect onEscherichia colimembranes

Involvement of reactive oxygen species in the cytotoxic effect of acid-electrolyzed water

Contact Info

Product

Resources

About