Effects of the Smad4 C324Y mutation on thyroid cell proliferation

Abstract

Smad4 is a key mediator of the transforming growth factor-β (TGF-β) superfamily that is involved in the control of cell proliferation and differentiation. We recently demonstrated that a Smad4 mutation, Smad4 C324Y, isolated from nodal metastases of papillary thyroid carcinoma, causes an increase of TGF-β signaling, responsible for the acquisition of transformed phenotype and invasive behaviour in thyroid cells stably expressing this mutation. In this paper, we demonstrate that the stable expression of Smad4 C324Y mutation in FRTL-5 cells is responsible for TSH-independent growth ability. Our data show that the Smad4 C324Y mutation interacts with P-Smad3 more strongly than Smad4 wt, already in basal condition; this interaction is responsible for TGF-β signaling and PKA activation that, in turn, determines an increased phosphorylation of CREB, necessary for the mitogenic actions of TSH. The expression of cyclin D1 also increases in all cells overexpressing the Smad4 C324Y mutation. All together, these data demonstrate that Smad4 C324Y mutation, interacting with the PKA pathway, gives cells the ability to proliferate independently from TSH.

miR-452-3p inhibited osteoblast differentiation by targeting Smad4

“…The Sry-related transcription factor Sox9, activator protein 1 (AP-1), runt-related transcription factor 2 (Runx2/Cbfa1), nuclear factor of activated T-cell cytoplasmic 1 (NFATc1) and twist are important in the process of osteogenic differentiation (Hsu, Chen & You, 2017). Smad4 is a key mediator of the transforming growth factor-β (TGF-β) pathway, which regulates cell proliferation, differentiation, and death (D'Inzeo et al, 2013). Previously, it was noted that smad4 is the key component of the TGF-β pathway and functions as a tumour suppressor (Yang & Yang, 2010).…”