Effects of the Solvent 1,2,4‐Trimethylcyclohexane on Respiratory Burst in Human Neutrophil Granulocytes: A Chemiluminescence and Electron Paramagnetic Resonance Spectrometry Study

The Formation of Free Radicals in Human Granulocytes and Cerebellar Granule Cells

“…Another advantage is that DEPMPO-OOH has a 15-fold longer half-life than DMPO-OOH. DEPMPO has successfully been used to detect both O 2 •and HO• in human granulocytes (9). The success of the spin-trapping experiment depends crucially on the local concentration of the spintrap.…”

“…The three methods described above were used to measure free radicals and ROS/RNS production in human granulocytes (3,7,9), detached nerve terminals (synaptosomes; 12, Myhre & Fonnum, unpublished results), and cultured cerebellar granule cells (Dreiem & Fonnum, unpublished results, Myhre & Fonnum, unpublished results). The biological preparations were exposed to organic hydrocarbon solvents or to the environmental toxicants, polychlorinated biphenyls (PCBs).…”