Effects of the time intervals between venipuncture and serum preparation for serum peptidome analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Identification of vitronectin as a novel serum marker for early breast cancer detection using a new proteomic approach

“…Sample collection and handling procedures can have a profound impact on serum proteome and peptidome patterns (Kulasingam and Diamandis 2008). Therefore, serum samples were collected under standardized conditions, as we reported previously (Umemura et al 2009). Blood samples were collected in vacutainer tubes (InsepakII, Sekisui Kagaku Kogyo, Tokyo, Japan).…”

Evaluation of serum carbohydrate-deficient transferrin by HPLC and MALDI-TOF MS

“…All serum samples were collected and processed using a previously described protocol [19] and stored at −80°C in aliquots until analysis. All subjects provided written informed consent and the Ethics Committee of Chiba University School of Medicine approved this study.…”

Identification of a high molecular weight kininogen fragment as a marker for early gastric cancer by serum proteome analysis

“…Serum samples were collected under standardized conditions as reported previously [15]. The obtained sera were m mucosal, sm submucosal, mp muscularis propria, ss subserosa, se serosa exposed, si serosa infiltrating divided into three aliquots, which were then placed in SUMILON Proteosave Ò SS 1.5-ml tubes (Sumitomo Bakelite, Tokyo, Japan) and stored at -80°C until analysis.…”

“…We recently demonstrated that time intervals between venipuncture and serum preparation had remarkable effects on serum peptidome patterns [15].…”