Effects of Therapeutical and Reduced Levels of Antibiotics on the Fraction of Antibiotic-Resistant Strains of <i>Escherichia coli</i> in the Chicken Gut

Horst, Michael; Fabri, T.; Schuurmans, J. Merijn; Koenders, Belinda B.; Brul, Stanley; Kuile, Benno H. ter

doi:10.1089/fpd.2012.1217

Cited by 15 publications

(7 citation statements)

References 16 publications

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“…The results obtained show a significant increase by 16-fold in MIC values of amoxicillin for E. coli isolated from the group that received amoxicillin. These results are similar to those obtained by Van der Horst et al who showed that the addition of amoxicillin, enrofloxacin, or oxytetracycline in the chicken's drinking water induced the selection for resistant E. coli [ 32 ]. Similarly, Miranda et al and Simoneit et al reported that the administration of amoxicillin or other antibiotics in drinking water induced the selection and development of antimicrobial resistance in E. coli strains from chickens [ 33 , 34 ].…”

Section: Discussionsupporting

confidence: 89%

In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

Hriouech

Akhmouch

Tanghort

et al. 2020

Veterinary Medicine International

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This study aims at verifying, in vitro, the extent to which the use of amoxicillin or thymol induces the selection of resistant bacteria and at evaluating in vivo their effects on the development of antimicrobial resistance in the intestinal flora of poultry. E. coli strain was subcultured on agar plates containing increasing concentrations of either amoxicillin or thymol. Thereafter, minimal inhibitory concentrations (MICs) of thymol, amoxicillin, and two other antibiotics, tylosin and colistin, were determined using the microdilution method. Groups of chicks were subjected to a 2-week regime of either amoxicillin or thymol added to their drinking water. During the treatment with either thymol or amoxicillin, the total aerobic mesophilic flora (TAMF) was counted on thymol-gradient plates or amoxicillin-gradient plates and the MICs of antibiotics and thymol for E. coli isolates were determined. The in vitro test showed that for E. coli, which had been serially subcultured on increasing concentrations of amoxicillin, a 32-fold increase in MIC values for amoxicillin and a 4-fold increase for colistin and tylosin were noted. However, the MIC of thymol for this strain remained constant. For the E. coli, which had been serially subcultured on increasing concentrations of thymol, no change in the MIC values for antibiotics and thymol was observed. The in vivo test confirmed the in vitro one. It demonstrated that exposure to amoxicillin induced a selection of antimicrobial resistance in TAMF and intestinal E. coli, whereas exposure to thymol did not. The results showed that the group receiving thymol had a lower consumption index compared to the other groups. This study demonstrates the feasibility of this natural product as an alternative solution to the current use of antibiotics in poultry farming.

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Section: Discussionsupporting

confidence: 89%

In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

Hriouech

Akhmouch

Tanghort

et al. 2020

Veterinary Medicine International

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“…The exposure to concentrations that simulate those during antibiotic therapy for a similar amount of time led to a consistent and lasting increase in the MIC, severely altering the susceptibility of the strain. A similar conclusion was reached in an in vivo situation when studying flock treatment of chickens [ 31 ]. Obviously, the best way to prevent these course of events is to render the initial treatment fail-proof by increasing the concentration to a level that ascertains the death of all pathogens.…”

Section: Discussionsupporting

confidence: 75%

Experimental Simulation of the Effects of an Initial Antibiotic Treatment on a Subsequent Treatment after Initial Therapy Failure

et al. 2014

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Therapy failure of empirical antibiotic treatments prescribed by primary care physicians occurs commonly. The effect of such a treatment on the susceptibility to second line antimicrobial drugs is unknown. Resistance to amoxicillin was rapidly induced or selected in E. coli at concentrations expected in the patient’s body. Strains with reduced susceptibility outcompeted the wild-type whenever antibiotics were present, even in low concentrations that did not affect the growth rates of both strains. Exposure of E. coli to amoxicillin caused moderate resistance to cefotaxime. The combined evidence suggests that initial treatment by amoxicillin has a negative effect on subsequent therapy with beta-lactam antibiotics.

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“…The observed increase in ampicillin resistance in broilers after amoxicillin treatment was in accordance with findings in previous studies. 11 , 24 Resistance rates to agents from other antibiotic classes (tetracyclines, aminoglycosides, sulfonamides, quinolones, and macrolides) also increased. Similarly, Jiménez-Belenguer et al 24 found increased resistance to tetracycline, aminoglycosides, and chloramphenicol after amoxicillin treatment.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance in Escherichia coli from Broiler Chickens After Amoxicillin Treatment in an Experimental Environment

Burow

Grobbel

Tenhagen

et al. 2020

Microbial Drug Resistance

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Groupwise antibiotic treatments are common in broiler chicken production. They induce selection for antibiotic resistance in commensal Escherichia coli. This study aimed to investigate antibiotic resistance after individual (I, drenching) or groupwise treatment (G, by water) with amoxicillin, and after contact with I or G (KI or KG), compared with untreated broilers without contact with treated broilers (C), and pretreatment values. Finally, we compared antibiotic resistance from broilers (G) after a second treatment, with a treatment in the contact animals (KG), and a first treatment in the control animals (C). Resistance to ampicillin and other antibiotics was significantly increased in groups G and I within 2 days, suggesting (co-)selection of resistance. The increase was lower in groups KI, KG, and C during the first treatment (days 1-5). The increased resistance in group C was interpreted as a change in the microbiota after initial moving and first feeding. After treatment, resistance rates decreased to initial or lower values in all groups. During the second treatment period (days 34-38), all three groups' (G, KG, and C) resistance levels increased to equally high levels. Cephalosporin resistance was low, and did not change over the experimental period. On days 3 and 38, resistance rates of E. coli from duodenum, jejunum, and cecum did not differ between segments and treatment routes. Overall, the baseline levels of antibiotic resistance in E. coli were high. Amoxicillin triggered an increase in resistance levels, irrespective of the mode of treatment. Substantial resistance dynamics in untreated controls warrant further investigation.

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Effects of Therapeutical and Reduced Levels of Antibiotics on the Fraction of Antibiotic-Resistant Strains of Escherichia coli in the Chicken Gut

Cited by 15 publications

References 16 publications

In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

In Vitro and In Vivo Comparison of Changes in Antibiotics Susceptibility of E. coli and Chicken’s Intestinal Flora after Exposure to Amoxicillin or Thymol

Experimental Simulation of the Effects of an Initial Antibiotic Treatment on a Subsequent Treatment after Initial Therapy Failure

Antibiotic Resistance in Escherichia coli from Broiler Chickens After Amoxicillin Treatment in an Experimental Environment

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