Effects of three blood derived products on equine corneal cells, an in vitro study

Abstract

The results demonstrate beneficial effects of PRP on proliferation as well as migration capacity of equine corneal cells in vitro. In vivo studies are warranted to determine further beneficial effects of PRP in horses with corneal ulcers.

PRGF in equine corneal cells: A standardised protocol is the key to achieve accurate results

“…at BTI Biotechnology Institute, a biomedical company that investigates in the fields of oral implantology and PRGF‐Endoret technology, we read with great interest the article by Rushton et al . regarding the assessment of three haemoderivatives on two types of equine primary ocular surface cells including corneal limbal cells and stromal cells. The blood‐derived products evaluated were autologous serum (AS) and two types of platelet‐rich plasma obtained with two different technologies: E‐PET™ (described in Rushton's article as PRP) and Endoret ® (described in Rushton's article as PRGF).…”

“…First of all, with regard to the PRGF obtaining protocol described in the work , we would like to remind the researchers that the centrifugation parameters should be specified as “relative centrifugal force” (g), and in case of indicate revolutions per minute (rpm), the radius of rotation (maximum) should be included. The accurate report of the centrifugation parameters is critical to compare this study with other protocols and to be able to reproduce the obtained data .…”

“…Although it is difficult to know what is the exact g‐force used to obtain PRGF, the ENDORET human protocol has been widely referenced by our group and others in g‐force value and converted to RPM as 1800 . According to this, we could suppose that the PRGF samples were obtained following the human protocol . However, it is widely described in the literature that the size of equine platelets is smaller than the human ones.…”

“…Third, there is a key point that may prejudge the outcomes of the study , since platelet activation with calcium chloride was not carried out, being an essential step in the protocol. Platelet activation release a wide range of growth factors and proteins related to tissue regeneration to the milieu .…”

“…On the other hand, although platelet concentration is a key parameter in the haematological analysis of all PRP types, no platelet data were included in the study. Furthermore, leucocyte count was described in table 1 providing leucocyte data for serum and PRGF samples in which the precipitates were counted as cells. Although, Rushton et al .…”