Effects of three commercial vaccines against porcine parvovirus 1 in pregnant gilts

Noguera, Marta; Vela, Antonio; Kraft, Christian; Chevalier, Mathieu F.; Goutebroze, Sylvain; Paz, Xavier de; Kunze, Marius; Rathkjen, P. H.; Schacht, Erik; Garcia-Morante, Beatriz

doi:10.1016/j.vaccine.2021.05.042

Cited by 5 publications

(10 citation statements)

References 30 publications

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“…While we could demonstrate the absence of PPV Abs by HI in most gilts after receiving the basic vaccination with vaccine 1 or 3, a statement on the seroconversion rate in gilts vaccinated twice with vaccine 2 cannot be provided due to the presence of high Ab titres indicating a previous infection with PPV [ 5 ].. Similar to our results, low seroconversion rates by ELISA in gilts vaccinated twice with vaccine 1 or vaccine 3 were already reported before [ 21 ]. Van den Born also reported that PPV Abs were detected by HI assay in only one third of all gilts vaccinated with vaccine 1 [ 18 ].…”

Section: Discussionsupporting

confidence: 81%

“…In addition, faecal samples were collected from the pen floors housing four-months-old fattening pigs in each farm to evaluate faecal shedding and consequently potential virus circulation in the farms. Since there is evidence that most gilts get infected with PPV prior to basic vaccination, interference of maternally derived antibodies (MDA) with antibodies due to active immunization cannot be excluded [ 21 ]. Therefore, knowledge on the actual time point of exposure to PPV and the duration of MDA in replacement gilts is pivotal for establishment of appropriate vaccination regimes.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, we also aimed to evaluate the duration of MDA in those farms. In general, studies on the duration of MDA are inconsistent [ 15 , 21 , 22 ]. While it was originally demonstrated that PPV induced MDA last approximately until the fifth to sixth month of life, Gava et al reported that MDA were no longer detectable by ELISA in serum samples of twelve-weeks-old pigs [ 15 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Duration of maternally derived antibodies of porcine parvovirus in growing pigs and presence of antibodies in gilts and sows vaccinated with three different parvovirus vaccines

Renzhammer,

Truyen,

Buchebner

et al. 2024

Porc Health Manag

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While gilts and sows are regularly vaccinated against the porcine parvovirus (PPV), little is known on the presence of antibodies in vaccinated sows nor the decline of maternally derived antibodies (MDA) in their offspring. On twelve farms serum samples were taken from 180 gilts and sows vaccinated at least twice with one of three different commercial PPV vaccines. On nine farms, additional 270 serum samples were collected from growing pigs of three different age categories. All 450 samples were examined for PPV antibodies (Abs) by ELISA and haemagglutination inhibition (HI) assay. In total, 65% of all gilts vaccinated twice with either vaccine 1 or vaccine 3 were seronegative by HI assay. In each farm, there were at least three animals with high Ab titres (≥ 1:1280) indicating the presence of PPV in all twelve study farms. However, PPV DNA could not be detected in collected faecal samples. While low to moderately high Ab titres (1:10–1:640) were measured in 98% of twelve-weeks-old pigs, ELISA was only positive in 30% of the same pigs. Though, the statement on the duration of MDA may depend on the applied test, we could confirm an exponential decay of MDA. In addition, we could demonstrate that applied serological tools are insufficient for the confirmation of successful vaccination.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Duration of maternally derived antibodies of porcine parvovirus in growing pigs and presence of antibodies in gilts and sows vaccinated with three different parvovirus vaccines

Renzhammer,

Truyen,

Buchebner

et al. 2024

Porc Health Manag

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“…In fact, several products from this expression system have been approved by the EMA and FDA to fight against main viral pathogens of swine (African swine fever virus/ASFV, Porcine circovirus 2/PCV-2 and Ungulate protoparvovirus 1/PPV) [147]. The composition of the vaccine products for ASFV is based on subunits of glycoprotein E2 of the outer envelope of the virus (Procilis ® Pesti and Bayovac CSF E2 ® ) [148] while the ones for PCV-2 and PPV are VLPs of capsid protein VP2 for PCV-2 (Ingelvac CircoFlex ® , Porcilis ® PCV, Circogard ® , and Circumvent ® PCV) [149][150][151], and the corresponding VP2 for PPV (Reprocyc ® ParvoFLEX) [152]. The initial approvals of recombinant vaccines for veterinary use were based on subunits, moving to multiprotein complexes as VLPs.…”

Section: Insect Cellsmentioning

confidence: 99%

Recombinant vaccines in 2022: a perspective from the cell factory

et al. 2022

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The last big outbreaks of Ebola fever in Africa, the thousands of avian influenza outbreaks across Europe, Asia, North America and Africa, the emergence of monkeypox virus in Europe and specially the COVID-19 pandemics have globally stressed the need for efficient, cost-effective vaccines against infectious diseases. Ideally, they should be based on transversal technologies of wide applicability. In this context, and pushed by the above-mentioned epidemiological needs, new and highly sophisticated DNA-or RNA-based vaccination strategies have been recently developed and applied at large-scale. Being very promising and effective, they still need to be assessed regarding the level of conferred long-term protection. Despite these fast-developing approaches, subunit vaccines, based on recombinant proteins obtained by conventional genetic engineering, still show a wide spectrum of interesting potentialities and an important margin for further development. In the 80’s, the first vaccination attempts with recombinant vaccines consisted in single structural proteins from viral pathogens, administered as soluble plain versions. In contrast, more complex formulations of recombinant antigens with particular geometries are progressively generated and explored in an attempt to mimic the multifaceted set of stimuli offered to the immune system by replicating pathogens. The diversity of recombinant antimicrobial vaccines and vaccine prototypes is revised here considering the cell factory types, through relevant examples of prototypes under development as well as already approved products.

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“…Variant 27a was suggested to be strongly virulent, immunologically “special”, cannot be neutralized by either homologous or heterologous sera, and commercial vaccines may not be fully protective against this genotype [ 7 , 8 , 10 ]. However, further direct experimental investigations of such vaccines were not able to confirm this idea [ 11 , 12 , 13 , 14 ]. Recently, much less significant serological differences of 27a from other PPV strains were reported [ 15 ] than were demonstrated earlier [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Allele-Specific Dual PCRs to Identify Members of the 27a Cluster of PPV

Tamás

Mészáros

Olasz

et al. 2022

Viruses

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Porcine Parvovirus (PPV) is one of the most important infectious agents causing severe reproductive failure in pigs. In the last two decades a particular, a novel genotype emerged in Europe and PPV-27a was named as the prototype of this genetic cluster. It was suggested that members of the PPV-27a cluster may adversely influence effective vaccination against PPV. For a reliable updated 27a definition, we aligned 93 databank-deposited partial or full nucleotide and protein sequences of the VP2 of different PPV isolates. We confirmed that the 27a cluster could indeed be distinguished from other members of the species, however, some divergences were identified compared to earlier defined genetic markers. Based on genetic differences, we developed a dual allele-specific polymerase chain reaction for the easy and quick discrimination of members of the 27a cluster from other PPV strains. The detection limit of dual PCR was found <1.66 × 104 copies/reaction. To sensitize and make it more user friendly, the method was further developed for qPCR application with fluorescent probes. Regarding the detection limit of the two PCRs (<1.66 × 104 copies/reaction of the dual PCR versus <2.40 × 102 copy/reaction of the dual qPCR), approximately two log improvement was achieved in the sensitivity of the method.

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Effects of three commercial vaccines against porcine parvovirus 1 in pregnant gilts

Cited by 5 publications

References 30 publications

Duration of maternally derived antibodies of porcine parvovirus in growing pigs and presence of antibodies in gilts and sows vaccinated with three different parvovirus vaccines

Duration of maternally derived antibodies of porcine parvovirus in growing pigs and presence of antibodies in gilts and sows vaccinated with three different parvovirus vaccines

Recombinant vaccines in 2022: a perspective from the cell factory

Allele-Specific Dual PCRs to Identify Members of the 27a Cluster of PPV

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