“…In Europe, in contrast, the critical N concentrations in high-yielding winter wheat were 3.24% at GS 31 and 1.70% at GS 64 (Justes, Mary, Meynard, Machet, & Thelier-Huche, 1994). It is likely the higher concentration values at GS 31 in Europe was due to the lower plant aboveground biomass associated with the low number of stems (Engstrom & Bergkvist, 2009;Greenwood, Neeteson, & Draycott, 1986). Similarly, the lower N concentration at GS 64 in Europe than on the NCP was likely Crop Science F I G U R E 2 Across three growing seasons (2015, 2016, and 2017), the relationship between biomass per stem and the N concentration of aboveground stems at (a-c) the beginning of the spring growth stage (Zadoks Growth Stage [GS] 25), (d-f) the stem elongation stage (GS 31), and (g-i) the anthesis stage (GS 64).…”