Effects of Thymoquinone on Sperm Parameters, Apoptosis, Testosterone Level, and Oxidative Stress in a Mouse Model of D-Galactose-Induced Aging

Attari, Seyedeh Saleheh; Mohammadi, Shabnam; Ebrahimzadeh, Alireza; Hosseinzadeh, Hossein; Soukhtanloo, Mohammad; Rajabzadeh, Aliakbar

doi:10.15171/ps.2018.26

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…Human studies revealed that Nigella sativa seed extract is a practical approach to improve sperm motility [ 13 ]. Moreover, low doses of TQ (5–10 µg/mL) can increase sperm motility in culture media in normozoospermic men [ 45 ] and in aging mice [ 46 ]. In contrast, Iranpour et al [ 20 ] clarified that the high levels of TQ (20 µg/mL) could ultimately impair the sperm motility in culture media.…”

Section: Discussionmentioning

confidence: 99%

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Khalil,

Hassan,

El-Harairy

et al. 2023

Animals

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Thymoquinone nanoparticles (TQNPs) are broadly utilized in numerous pharmaceutical applications. In the present study, we tested the effects of TQNP supplementation on sperm quality and kinematics, acrosome exocytosis, oxidative biomarkers, apoptosis-like and morphological changes of frozen–thawed buffalo sperm, as well as the fertilizing capacity. Semen was collected from buffalo bulls, diluted (1:10; semen/extender), and divided into five aliquots comprising various concentrations of TQNP 0 (CON), 12.5 (TQNP12.5), 25 (TQNP25), 37.5 (TQNP37.5), and 50 (TQNP50) µg/mL, and then cryopreserved and stored in liquid nitrogen (−196 °C). The results revealed that TQNPs (25 to 50 µg/mL) provided the most optimal results in terms of membrane integrity (p < 0.001) and progressive motility (p < 0.01). In contrast, TQNP50 resulted in a greater post-thawed sperm viability (p = 0.02) compared with other groups. The addition of TQNPs to the extender had no discernible effects on sperm morphology measures. Sperm kinematic motion was significantly improved in the TQNP50 group compared to the control group (p < 0.01). TQNPs effectively reduced the content of H2O2 and MDA levels and improved the total antioxidant capacity of post-thawed extended semen (p < 0.01). The addition of TQNP significantly increased the number of intact acrosomes (p < 0.0001) and decreased the number of exocytosed acrosomes (p < 0.0001). A significant reduction in apoptosis-like changes was observed in TQNP groups. The non-return rates of buffalo cows inseminated with TQNP50-treated spermatozoa were higher than those in the control group (p < 0.05; 88% vs. 72%). These findings suggested that the freezing extender supplemented with TQNPs could effectively enhance the cryotolerance and fertility of buffalo sperm.

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Section: Discussionmentioning

confidence: 99%

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Khalil,

Hassan,

El-Harairy

et al. 2023

Animals

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“…After cooling, it was centrifuged at 1,000 x g for 10 min and then absorbed at 535 nm was read by a spectrophotometer and the malondialdehyde (MDA) concentration was calculated in nmol/g (Attari et al, ).…”

Section: Methodsmentioning

confidence: 99%

Effects of dioxin on testicular histopathology, sperm parameters, and CatSper2 gene and protein expression in Naval Medical Research Institute male mice

et al. 2019

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The CatSper gene family is known to be solely expressed in sperm cells and is possibly associated with sperm motility and penetration through the zona pellucida. Despite its vital role in male fertility, factors regulating its expression are not widely known. The present study aimed at evaluating the effects of dioxin on CatSper2 gene and protein expression, testicular histopathology, sperm quality and biochemical parameters in a mice model. The experiments were performed on 32 Naval Medical Research Institute male mice (2–3 months). The animals were divided into four groups in a random manner: (a) control; (b) dioxin 1; (c) dioxin 2; and (d) dioxin 3. The treatment groups received 0.1, 0.5 and 1 µg/kg of dioxin intraperitoneally every day for 2 weeks. Administration of dioxin significantly downregulated the CatSper2 gene and protein expression. A greater reduction in gene and protein expression was found at higher doses of dioxin. At the same time, sperm parameters, especially sperm motility and count, decreased in mice exposed to dioxin. The results of testicular histopathology showed necrotic degeneration and epithelium thickness reduction in the dioxin groups in comparison with the controls. Besides, oxidative stress increased in seminiferous tubules.

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“…After infiltrating with paraffin in 60º C , the molds were sectioned using rotary microtome. Hematoxylin-Eosin staining (Merck, Germany) was used for examining the samples and was investigated with optical microscopy [14].…”

Section: Histopathological Study Of Testicular Tissuementioning

confidence: 99%

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

Mohammadi

Beheshti

Elyasi

et al. 2020

JPRI

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The aim of this study was to investigate the effects of propranolol on sperms, histopathology of testes, and CatSper 2 gene and protein expression in adult mice. 18 adult male mice were randomly divided into control, propranolol 1 (receiving 10 mg/kg dose) and propranolol 2 group (receiving 15 mg/kg dose for 35 days). The mean amount of sperm parameters in the propranolol 1 and propranolol 2 groups was significantly lower than the control group (p<0.05). CatSper2 gene and protein expression have significantly decreased in propranolol 1 and propranolol 2 groups compared to the control group (P<0.05). Reduction of CatSper2 gene and protein expression in low dose of propranolol was more severe than high dose. In testicular tissues of the propranolol 1 group, vacuoles and necrosis in the germinal epithelium were observed, and in testicular tissues of propranolol 2 group decrease in the thickness of the germinal epithelium, some vacuoles and necrosis were observed in germinal epithelium as well as congestion in the interstitial space. The mean value of thiol and catalase enzyme in the propranolol 1 and propranolol 2 groups, and the mean value of superoxide dismutase in propranolol 1 group, were significantly different compared to the control group (P<0.05).

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Effects of Thymoquinone on Sperm Parameters, Apoptosis, Testosterone Level, and Oxidative Stress in a Mouse Model of D-Galactose-Induced Aging

Cited by 8 publications

References 11 publications

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Effects of dioxin on testicular histopathology, sperm parameters, and CatSper2 gene and protein expression in Naval Medical Research Institute male mice

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

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