Effects of thyroliberin (TRH) on cell proliferation and prolactin secretion by GH3/B6 rat pituitary cells: A comparison between serum‐free and serum‐supplemented media

Brunet, N.; Rizzino, Angie; Gourdji, D.; Tixier‐Vidal, A.

doi:10.1002/jcp.1041090220

Cited by 18 publications

(8 citation statements)

References 32 publications

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“…As for the GH cell line, it was shown that EGF stimulated prolactin (PRL) production and inhibited growth hormone (GH) production accompanied by the inhibitory action on cellular growth (Johnson et al, 1980;Schonbrunn et al, 1980). Similar effects on cell proliferation and hormone production have also been reported with thyrotropin-releasing hormone (TRH), which regulates the GH cell line (Schonbrunn et al, 1980;Brunet et al, 1981). TRH stimulates the PRL production, which inhibits the GH production, and also suppresses the rate of proliferation of GH3 cells.…”

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confidence: 84%

The effects of epidermal growth factor on cell proliferation and prolactin production by GH₃ rat pituitary cells

Yajima

Saito

1984

Journal Cellular Physiology

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The effects of epidermal growth factor (EGF) were studied in rat pituitary tumor cells, GH3, grown in serum-supplemented and serum-free chemically defined media. EGF (1 nM) increased the cell number to 132% of the control cultured in the defined medium during a 6-day incubation period, while it decreased the cell number to 60% of the control in the serum-supplemented medium. EGF altered the morphology of the cells grown in the defined medium more markedly to an elongated conformation than that of cells grown in the serum-supplemented medium. EGF also stimulated prolactin (PRL) production by culture in the presence or absence of serum. The effects of the cell density of GH3 on the action of EGF were shown to appear in two ways. The mitogenic influence of EGF was more effective on, and more responsive to, high-density cells, whereas the stimulatory action on PRL production was less effective on high-density cells. However, the inhibitory effects on cellular growth appeared independently of cell densities. The results obtained with 125I-EGF binding experiments indicated that the number of binding sites, affinity, and internalization of EGF receptors were similar in either serum-supplemented or serum-free culture. At low cell density, the number of available 125I-EGF binding sites per cell was larger than at high cell density. These results suggested that there was no apparent correlation between EGF binding and its differing effects on the growth of GH3 cultured in the serum-supplemented and the defined medium.

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confidence: 84%

The effects of epidermal growth factor on cell proliferation and prolactin production by GH₃ rat pituitary cells

Yajima

Saito

1984

Journal Cellular Physiology

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“…TRH regulates the synthesis and secretion of TSH and PRL by thyrotroph and lactotroph cells, respectively (30). In addition, TRH regulates proliferation of specific pituitary cells (31,32) and has been implicated in the formation of pituitary adenomas (33). TRH binds and activates two G protein-coupled receptors, TRHR1 and TRHR2 (34), which transduce signals predominantly via the PKC and mitogen-activated protein kinase (MAPK) pathways (35)(36)(37).…”

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confidence: 99%

Thyrotropin-releasing Hormone and Epidermal Growth Factor Regulate Iron-regulatory Protein Binding in Pituitary Cells via Protein Kinase C-dependent and -independent Signaling Pathways

Thomson

Rogers

Leedman

2000

Journal of Biological Chemistry

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“…Clausen et al (1978) reported that, using microfluorometric ana¬ lysis, TRH decreased the number of cells in the S phase by about 40% while increasing the number of cells in Gi. It has also been reported that TRH showed a slight suppression of the growth of subclones of GH3 cell lines in the medium supple¬ mented with serum (Schonbrunn et al 1980;Brunet et al 1981). Our results are compatible with these findings.…”

Section: Discussionmentioning

confidence: 95%

The effects of hypothalamic extracts and TRH on the growth and hormone production of GH3 cells in culture

Yajima

Saito

1983

Acta Endocrinologica

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Hypothalamic factors were tested for their effects on the production of hormones and the growth of GH3 cells, cloned rat pituitary cells producing prolactin (Prl) and growth hormone (GH). Hypothalamic extracts (HE) (0.05 mg/ml) and TRH (0.3 \g=m\m)stimulated the synthesis of Prl to levels of 306% and 360%, respectively, of the control culture in a medium containing 0.5% foetal bovine serum (FBS) during a 24 h incubation. They did not affect the rate of GH production. The thymidine uptake was suppressed to 57% and 46% of the control by the addition of HE and TRH, respectively. They also inhibited the growth of GH3 to 70% and 74% of the control culture during an 8-day incubation period.On the other hand, LRH affected neither the rate of hormone production nor the thymidine uptake. Somatostatin suppressed the synthesis of Prl and GH, but it did not affect the incorporation of thymidine into the cells. The gel filtration studies of HE revealed that the inhibitory effects of HE on the thymidine uptake were dependent on two substances, TRH and an unknown factor(s) of high molecular nature. The relationship between hormone synthesis and DNA synthesis will be discussed on the basis of the TRH-induced effects on Prl production and DNA synthesis in GH3 cells.Hormonal factors that stimulate the secretion of hormones by endocrine glands or their tumours are generally considered to stimulate proliferation of these cells, as observed in the effect of ACTH upon the adrenal cortex and TSH upon the thy¬ roid gland. As for TRH, it has been reported that it affected the growth of a rat pituitary tumour cell 1 Requests for reprints: The Tokyo Metropolitan Institute of Medical Science, 3-18, Honkomagome, Bunkyoku, Tokyo, 113 Japan. line GH3, a multi hormone-producing cell strain (Hayashi et al. 1978; Clausen et al. 1978; Schon¬ brunn et al. 1980; Brunetetal. 1981). On the other hand, it is not yet known whether hypothalamic factors other than TRH which have either stimula¬ tory or suppressive effects on the release of hor¬ mones from the pituitary gland may affect the growth of this tissue. In the present investigation, the effects of hypo¬ thalamic factors, TRH, LRH, somatostatin and hypothalamic extracts on the proliferation of cells were examined using GH3 cells in comparison with the effects on the release of GH and Prl. Materials and Methods Media and Hormones Tissue culture media, horse serum and foetal bovine serum (FBS) were obtained from Flow Laboratories Co. (Rockville, MD). Synthetic TRH and LRH were gifts from Tanabe Pharmaceutical Co. (Osaka, Japan), and somatostatin and triiodothyronine (T3) were purchased from Sigma Chemical Co. (St. Louis, MO). Rat hypo¬ thalamic extracts (HE) and kits for radioimmunoassay of rat prolactin (Prl) and rat growth hormone (GH) were supplied by the National Institute of Arthritis, Metabo¬ lism and Digestive Disease, USA. Methyl[3H]thymidine, 20 Ci/mmol, and L-proline-2,3,4,5-[3H(N)]TRH, 100 Ci/ mmol, were from New England Nuclear Corp. (Boston, MA). Cell culture GH3 cells were obtained fro...

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Effects of thyroliberin (TRH) on cell proliferation and prolactin secretion by GH3/B6 rat pituitary cells: A comparison between serum‐free and serum‐supplemented media

Cited by 18 publications

References 32 publications

The effects of epidermal growth factor on cell proliferation and prolactin production by GH₃ rat pituitary cells

The effects of epidermal growth factor on cell proliferation and prolactin production by GH₃ rat pituitary cells

Thyrotropin-releasing Hormone and Epidermal Growth Factor Regulate Iron-regulatory Protein Binding in Pituitary Cells via Protein Kinase C-dependent and -independent Signaling Pathways

The effects of hypothalamic extracts and TRH on the growth and hormone production of GH3 cells in culture

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Effects of thyroliberin (TRH) on cell proliferation and prolactin secretion by GH3/B6 rat pituitary cells: A comparison between serum‐free and serum‐supplemented media

Cited by 18 publications

References 32 publications

The effects of epidermal growth factor on cell proliferation and prolactin production by GH3 rat pituitary cells

The effects of epidermal growth factor on cell proliferation and prolactin production by GH3 rat pituitary cells

Thyrotropin-releasing Hormone and Epidermal Growth Factor Regulate Iron-regulatory Protein Binding in Pituitary Cells via Protein Kinase C-dependent and -independent Signaling Pathways

The effects of hypothalamic extracts and TRH on the growth and hormone production of GH3 cells in culture

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The effects of epidermal growth factor on cell proliferation and prolactin production by GH₃ rat pituitary cells

The effects of epidermal growth factor on cell proliferation and prolactin production by GH₃ rat pituitary cells