Effects of tiletamine-zolazepam anaesthesia on plasma antioxidative status and some haematological parameters in sheep

Abstract

It is not clear whether the anaesthetic agents tiletamine and zolazepam have antioxidant or pro-oxidant effects. Therefore, this study was carried out to investigate the effects of tiletamine-zolazepam anaesthesia on oxidant/antioxidant status in blood plasma and on haematological parameters in 10 healthy Awassi ewes. The tiletamine-zolazepam combination was administrated in a dose of 7.5 mg/kg intramuscularly. The animals were spontaneously breathing air during the procedure. Blood samples were collected by jugular venipuncture before induction and at 30, 60, 120 min, 24 h and 3 days after anaesthesia. Malondialdehyde concentration, an index of lipid peroxidation, was higher at 30, 60, 120 min and 24 h (P < 0.05) than the baseline value in the plasma. The level of glutathione decreased (P < 0.05) at 30, 60 and 120 min, then returned to the baseline level. Beta-carotene concentration was lower (P < 0.05) than the baseline value during anaesthesia with the exception of its level at 120 min. Glutathione peroxidase and catalase activities decreased (P < 0.05) at the onset of anaesthesia, then returned to baseline values. There was no significant change in vitamin A level. Red blood cell count, haematocrit and haemoglobin concentration significantly decreased (P < 0.05) only at 30 min and thereafter they gradually returned to the baseline values. Based on the results tiletamine-zolazepam anaesthesia seems to accelerate lipid peroxidation and to impair the enzymatic antioxidant defence in the blood plasma.

Effects of the tiletamine/zolazepam-xylazine-tramadol combination on plasma oxidative status and haematological indicators in miniature pigs

“…However, other studies examining the use of tiletamine-zolazepam (Ceylan et al 2007) or its combination with xylazine in sheep (Aydilek 2007) and gazelles (Yaralioglu-Gurgoze et al 2005) demonstrated an increase in MDA concentrations, similar to the results of our study. Based on the fact that MDA is one of the end products of lipid peroxidation, and is both an indicator and effector of oxidative stress (Sivaci et al 2006) our study may support the fact that oxygen free radicals are released upon administration of TZXT.…”

Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs

“…Ceylan et al [7] found a decrease in hemoglobin 30 min after intramuscular injection of tiletamin and zolazepam to sheep, but the level was normalized after 60 min. In the time-course study, the hemoglobin did not return to baseline within the 105 min the study lasted; on the contrary, the hemoglobin continued to decrease, and after 30 min, a decrease was also observed in the non-anesthetized group.…”

Comparison between Xylazine-Tiletamine-Zolazepam and Fentanyl-Tiletamine-Zolazepam Anaesthetic Combinations on Plasma Oxidative Status in Sheep

“…Ceylan et al (2007b) reported that tiletamine-zolozepam anaesthesia alone induced lipid peroxidation in plasma of sheep. Similarly, a significant elevation in the MDA levels, index of lipid peroxidation in response to both anaesthetic administrations was noticed in the current study (Table 1).…”