Effects of TiO2 nanoparticles on the NO2 − levels in cell culture media analysed by Griess colorimetric methods

Popescu, Traian; Lupu, Andreea-Roxana; Diamandescu, L.; Tărăbăşanu-Mihăilă, D.; Teodorescu, V. S.; Rădiţoiu, Valentin; Purcar, Violeta; Vlaicu, A. M.

doi:10.1007/s11051-013-1449-0

Cited by 11 publications

(12 citation statements)

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“…Regarding the hydrothermal TiO 2 samples, although they exhibit larger surface specific area than Degussa P25, their in vitro reactivity appears to be reduced. This may be related to their different surface charge properties in culture media as well as ROS photogeneration capacities and hydrophilicity [20].…”

Section: Discussionmentioning

confidence: 99%

“…Degussa P25 TiO 2 was often tested (and utilized as reference material) in studies concerning intracellular ROS generation [16] and toxicity induced by titania nanomaterials [17][18][19]. The hydrothermal TiO 2 samples have similar structural characteristics (crystal structures, shapes, sizes and specific surface areas [20] -the relevance of these factors being frequently considered in TiO 2 nanotoxicology studies [21,22]), but different band gap energies (relative to each other) and colloidal behaviors (compared to Degussa P25) [20].…”

Section: +mentioning

confidence: 99%

“…The obtained results are analyzed with respect to the structural and physicochemical properties of the tested nanomaterials [20], the characteristics of the used cells and the cell penetration and hydroxyl radical scavenger properties of DMSO [23].…”

Section: +mentioning

confidence: 99%

“…The structural, morphological, optical and physicochemical characteristics of the used materials have been studied by X-ray diffraction (XRD), transmission electron microscopy (TEM), Energydispersive X-ray spectroscopy (EDX), Mössbauer spectroscopy, Brunauer-Emmett-Teller (BET) nitrogen adsorption, UV-Vis reflectance spectroscopy, Dynamic Light Scattering (DLS) and Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFT) and described in a previous work [20]. The experiments were such designed to elucidate the following aspects:…”

Section: Materials Characterizationmentioning

confidence: 99%

“…The detailed structural and physicochemical characterization of the three TiO 2 nanomaterials used in this study was published in a previous work [20]. Briefly, all three types of titania have similar shapes (no acicular shaped particles) and average particle sizes between 10-30 nm.…”

Section: Materials Characterizationmentioning

confidence: 99%

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Combined In vitro Effects of TiO2 Nanoparticles and Dimethyl Sulfoxide (DMSO) on HepG2 Hepatocytes

Lupu¹

2015

Int J Nanomater Nanotechnol Nanomed

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Introduction: Professional workers that manufacture or use titanium dioxide (TiO 2 )-based paints are exposed to potentially toxic TiO 2 nanomaterials as well as to different paint solvents such as dimethyl sulfoxide (DMSO). In this context, we evaluate the combined cytotoxic effects of TiO 2 nanoparticles and DMSO on HepG 2 human hepatocytes.Methods: Three types of TiO 2 nanoparticles were used: commercial Degussa P25 and two samples synthesized by a hydrothermal procedure -undoped and Fe 3+ -doped TiO 2 . The effects of TiO 2 nanoparticles on HepG2 cells exposed to DMSO before, after or together with the TiO 2 treatment were investigated by viability and intracellular reactive oxygen species (ROS) determinations, performed using the MTT and DCFH-DA(2',7'-dichlorfluorescein-diacetate) methods respectively. Results:Results indicated that DMSO made HepG2 cells more susceptible to toxic effects induced by nanosized TiO 2 . In the absence of DMSO, none of the tested nanoparticles exhibited significant cytotoxic effects. Viability increases were detected after 48 hours of treatment and attributed to possible redox-sensitive proliferation mechanism striggered by the low and moderate amounts of produced ROS. The combined action of TiO 2 and DMSO led to a general viability decrease tendency. Significant effects (viability reductions and ROS generation) were observed in the case of cells first treated with Degussa P25 TiO 2 and afterwards exposed to DMSO. The hydrothermal materials exhibited reduced in vitro reactivity on HepG 2 hepatocytes. Conclusion:The study reveals the enhancement of nanosized TiO 2 toxicity induced by DMSO exposure, its findings having potential to help in the evaluation of professional health risks associated to the combined action of TiO 2 nanomaterials and paint solvents.

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Section: Discussionmentioning

confidence: 99%